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ProductsAFP ELISA Test
Company Diagnostic Systems Laboratories, Inc.
  • No sample pre-treatment required
  • Only 25 uL sample needed
  • Incubation: 1 hr + 30 mins + 10 mins on shaker at RT
  • Description Alpha Fetoprotein (AFP) is a 68 kDa protein, which is produced primarily during fetal life by the fetal liver and yolk sac [1]. Human fetal serum AFP levels peak at ~14 weeks gestation, and production rates reach 19-26 g/min during mid-gestation (14-20 weeks). Although production rates and estimates of total fetal AFP continue to increase after mid-gestation, a gradual decline in body fluid concentrations occurs after mid-gestation, probably due to a dilutional effect. AFP is effectively cleared by the fetal kidney and is excreted into the amniotic fluid. AFP also appears in the maternal serum, presumably by transplacental transfer and thus AFP levels in amniotic fluid and maternal serum generally parallel those in fetal serum. During mid-gestation, AFP concentrations are in the order of mg/mL in fetal serum, g/mL in amniotic fluid and ng/mL in maternal serum. After birth, serum AFP levels decline rapidly during the first year of life and low basal levels are then apparently maintained throughout childhood and adult life [2]. The function of AFP is not well defined, however, measurement of AFP concentrations in body fluids has been useful in studies of fetal physiology and pathophysiology.

    1. Seppl M: Fetal pathopysiology of human a-fetoprotein. Ann NY Acad Sci 259:59-73, 1975.
    2. Masseyeff R, Gilli J, Krebs B, Calluaud A, Bonet C: Evolution of a -fetoprotein serum levels throughout life in humans and rats, and during pregnancy in the rat. Ann NY Acad Sci 259: 17-26, 1975.
    Info Diagnostic Systems Laboratories, Inc.
    Customer Service: (800) 231-7970
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