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Virus may Be the Cause of Mad Cow

ds, starting a cascade of abnormal changes in other proteins.

In Manuelidis' previous experiments, prions did not appear until late in the progression of spongy brain diseases.

To find what appears at an earlier phase, Manuelidis' team homogenized mouse brains infected with scrapie and Creutzfeldt-Jakob disease and injected them into nerve cell cultures.

The only new objects were dense spheres that looked like small viruses, she said.

She later added a compound to spur the growth of prions about fivefold to see if that would increase the level of infection. The level did not show a significant increase, suggesting that prions were not the active agent of infection, she said.

To prove that the virus-like particles are solely responsible for the infection, Manuelidis plans to isolate the particles and see if they can start an infection when injected into healthy cells.

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