Navigation Links
Vaccine to protect birds against avian flu, engineered

Researchers at the University of Pittsburgh said that they have genetically engineered an avian flu vaccine. The vaccine contains the components of the deadly strain H5N1.//

This vaccine in animal models offered complete protection to mice and chickens from infection. The vaccine contains a live virus, which is very efficient in producing an immune response than those prepared by traditional methods. It is cultured in cells and very easily produced in large amounts. It prevents the spread of the virus in domestic livestock populations and humans. The findings were published in the Journal of Virology.

Andrea Gambotto, M.D., assistant professor in the departments of surgery and molecular genetics and biochemistry, University of Pittsburgh School of Medicine, and lead author of the study said that the vaccine stimulates several lines of immunity against H5N1.

Dr. Gambotto and his colleagues genetically engineering a common cold virus (adenovirus) to express the avian influenza protein (hemagglutinin) (HA) on its surface. This virus then attaches to all the cells that are infected. Bird flu (H5N1) has affected a large number of birds in different areas like Cambodia, China, Indonesia, Japan, Laos, South Korea, Thailand and Vietnam, Turkey and Romania. Due to this 150 to 200 million birds have been culled to prevent the spread of infection.

The first incidence of bird flu attack in humans was in 1997 at Hong Kong. The virus causes severe respiratory illness resulting in death.

The adenovirus serves as vector or transporting vehicle for delivering the foreign genes or DNA of the HA protein.

Dr. Gambotto's team took two sets of mice in one set they injected the genetically engineered vaccine and in the other an adenovirus vector containing no H5N1 genes, or an empty vector. The mice immunized with the empty vector showed substantial weight loss and all were dead within six to nine days of avian flu exposure. On the other hand, most of the mice immunized with the adenovirus containing the HA protein showed only mild weight loss and survived H5N1 infection. It was also found that full length HA vaccine gave a complete protection against the H5N1 strain.

The mice which is immunized with full-length HA vaccine developed strong T-cell responses. Hence this recombinant vaccine offers a greater therapeutic value. Even if H5N1 mutates, the vaccine is still offers protection against the disease.

Based on the superior degree of protection that they found in mice vaccinated with full-length HA vaccine, Dr. Gambotto's group along with David E. Swayne, D.V.M., Ph.D., at the U.S. Department of Agriculture, tested the vaccine’s effectiveness in chickens. Chickens were injected with the vaccine and the empty vector both intranasally and subcutaneously. The chickens were then challenged with a dose of whole H5N1 virus 10,000 times greater than the dose given to the mice.

It was found that all of the chickens that were immunized subcutaneously survived exposure to H5N1 and developed strong HA-specific antibody responses and showed no clinical signs of disease. On the other hand 50% of those that were immunized intranasally died. All of the chickens immunized with the empty vector (intranasally and subcutaneously) died within two days of H5N1 exposure.

Dr. Gambotto and his colleagues suggest that these live vaccines can be used in combination with the traditional inactivated influenza vaccines.

Hence the research team concludes by saying that the new vaccine would have the ability to contain outbreaks of mutant strains. Further trails in the humans have to be conducted in order to understand the efficiency of the vaccine in humans.


'"/>




Related medicine news :

1. Vaccine to quit smoking?
2. First Vaccine Designed for Africa Cleared for Testing in Humans
3. Vaccine for Alzheimer’s diseas
4. Health Officials Recommend Flu Vaccine
5. Smallpox Vaccine May Help Fight Cervical Cancer
6. Panacea Biotech To Market Anthrax Vaccine
7. New Prostate Cancer Vaccine Shows Promise
8. Drug Firm Offers to Donate Smallpox Vaccine
9. Vaccine guards against shingles
10. Vaccine for septic conditions
11. A new Vaccine for Pneumonia
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... ... June 27, 2016 , ... ... technology to revolutionize the emergency ambulance transport experience for the millions of people ... how Uber has disrupted the taxi industry through the use of technology. Now, ...
(Date:6/26/2016)... ... June 26, 2016 , ... Pixel Film Studios Released ProSlice Levels, a Media ... give their videos a whole new perspective by using the title layers in ... Studios. , ProSlice Levels contains over 30 Different presets to choose from. ...
(Date:6/25/2016)... ... 2016 , ... Experts from the American Institutes for Research ... June 26-28, 2016, at the Hynes Convention Center in Boston. , AIR experts ... planning, healthcare costs and patient and family engagement. , AIR researchers will be ...
(Date:6/25/2016)... ... June 25, 2016 , ... First Choice Emergency Room , ... Sesan Ogunleye, as the Medical Director of its new Mesquite-Samuell Farm facility. , ... our new Mesquite location,” said Dr. James M. Muzzarelli, Executive Medical Director of First ...
(Date:6/25/2016)... ... ... Conventional wisdom preaches the benefits of moderation, whether it’s a matter of ... too high can result in disappointment, perhaps even self-loathing. However, those who set the ... Research from PsychTests.com reveals that behind the tendency to set low expectations ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016 The vast majority ... outpatient dialysis facility.  Treatments are usually 3 times a ... per visit, including travel time, equipment preparation and wait ... but especially grueling for patients who are elderly and ... skilled nursing and rehabilitation centers for some duration of ...
(Date:6/23/2016)... , June 23, 2016  Experian ... integrating and transforming the patient payment and ... several innovative new products and services that ... its revenue cycle offerings. These award-winning solutions ... efficient workflows, remain compliant in an ever-changing ...
(Date:6/23/2016)...  The National Pharmaceutical Council (NPC) today announced ... research organization as its newest member.  ... president and chief scientific officer, Mallinckrodt Pharmaceuticals, will ... Board of Directors. ... us in support of our efforts to conduct ...
Breaking Medicine Technology: