Navigation Links
Three-Dimensional Image Of AIDS Virus – BY FSU Researcher

Florida State University, has developed a three-dimensional image of the AIDS virus, with lots of protein spikes on its surface, which aids in fusing// with human immune cells – An extraordinary work since past 25 year when it was diagnosed.

Findings from this AIDS research could boost the development of vaccines that will thwart infection by targeting and crippling the sticky HIV-1 spike proteins. In fact, said principal investigator and FSU Professor Kenneth H. Roux, at least two laboratories already are crafting vaccine candidates based on preliminary results uncovered by his team of structural biologists.

Those results are described in the online edition of the journal Nature. Never before generated in such intricate detail, the super-sized images of the virus and its viral spikes have given researchers their first good look at the pathogen's complex molecular surface architecture that facilitates the infection process.

‘Until now, despite intensive study by many laboratories, the design details of the spikes and their distribution pattern on the surface of the virus membrane have been poorly understood, which has limited our understanding of how the virus infection actually occurs and frustrated efforts to create vaccines,’ Roux said.

To produce the images, research associate Ping Zhu, Roux, and their colleagues used a state-of-the art technique called cryoelectron microscopy tomography. It generates three-dimensional images similar to those from a CAT scan, but at the level of viruses and molecules rather than tissues and organs.

They imaged HIV samples as well as a mutant SIV (non-human primate) strain, genetically engineered for the study by collaborators at the National Cancer Institute to express about 74 spikes as opposed to the 14 found on the HIV virus –- more spikes make it easier to work with. The virus samples were suspended in a thin liquid film stretched across the holes of a small copper gri d and then flash-frozen, creating a solid form of ice that is more like clear glass than the typical crystalline form in ice cubes.

Once inside the electron microscope, electrons bombarded the samples from myriad angles, magnifying it more than 43,000 times to reveal its surprising structure –- absent the degree of distortion caused by the more typical imaging methods involving drying and staining of specimens.

As a result, the researchers were able to hone in on the envelope –- the lipid membrane covering the virus itself. They imaged the spikes protruding from the envelope, which contain the only viral protein molecules on the HIV surface. The FSU scientists also were able to capture super-sized images of both the head of the spike and its supporting stalk. The spike head is responsible for binding the virus to the target cell. Its stalk is responsible for the fusion event in which HIV injects its genes into the human host cells for which the virus has a natural affinity –- T lymphocytes and macrophages.

‘Antibodies that effectively bind to either of these spike parts will neutralize the virus to prevent infection,’ said Roux, a member of FSU's biological science faculty since 1978.

His biggest surprise: the stalk has legs.

‘Researchers thought the spike stalk was comprised of a tight collection of three rods bound together with the head of the spike perched on top. But our images reveal that the stalk is split into three legs, spread more like a tripod, which increases their contact with the viral membrane,’ Roux said. ‘Seeing the tripod stalk suggests a novel mechanism by which HIV-1 is able to so effectively fuse with our cells. That essential knowledge should help us design better weapons to fight the virus.’

FSU Arts and Sciences Dean Joseph Travis has declared the work ‘a beautiful example of what happens when strong, sound basic science is applied to a very difficult problem.’

The National Institutes of Health funded the two-year study, conducted by members of the department of biological science and the Institute of Molecular Biophysics at FSU.

AIDS has produced one of the worst pandemics ever known. About 25 million people have died and 40 million are infected worldwide –- including 1 million in the United States.

Source Eurekalert
'"/>




Related medicine news :

1. Parents Encourage Stereotype Body Images
2. Sexy Images and Lingerie Challenge A Man’s Decision Making Skill
3. Image-Guided Biopsy Can Alter Clinical Decisions In Kidney Patients
4. Coronal Multiplanar Reformatted Images to Replace the Conventional Ones
5. Do Food Images Encourage Binge Eating and Obesity ?
6. Body Image Reflects Mood Disorder In Adolescents - Study Finds
7. Satellite Imagery to monitor the Social Impact Of Geographical Changes
8. Sensor Images Show Soaring Temperature and Pollution Levels in UK
9. Brain Images Show Hysteria Not an Imaginary Disorder
10. 3 –D Image of Influenza Virus – A Definite Breakthrouh
11. New Computed Imaging Technique Uses Hazy Images to Improve View
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... ... October 12, 2017 , ... Information about the ... to develop to enable prevention of a major side effect of chemotherapy in ... in pediatric patients. For cisplatin, hearing loss is FDA listed on-label as a ...
(Date:10/12/2017)... ... October 12, 2017 , ... HMP , a ... of a 2017 Folio Magazine Eddie Digital Award for ‘Best B-to-B Healthcare Website.’ Winners ... October 11, 2017. , The annual award competition recognizes editorial and design excellence across ...
(Date:10/12/2017)... Wesley Chapel, FL (PRWEB) , ... October 12, 2017 , ... ... Chapel is holding a treadmill relay – Miles by Moonlight to raise money for ... by donating $300 or more. , Teams will work together to keep their ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... first interactive health literacy software tool, and the Cancer Patient Education Network (CPEN), ... patient education, today announce a new strategic alliance. , As CPEN’s strategic ...
(Date:10/12/2017)... ... 12, 2017 , ... Vohra Chief Medical Officer Dr. Shark ... skilled nursing facility medical directors and other clinicians at various events in October. ... "At many of these conferences we get to educate other physicians, facility nurses, ...
Breaking Medicine News(10 mins):
(Date:9/25/2017)... 25, 2017   Montrium , an industry ... today—from the IQPC Trial Master Files & Inspection ... that EastHORN Clinical Services has selected eTMF ... TMF management. EastHORN, a leading European contract research ... increase transparency to enable greater collaboration with sponsors, ...
(Date:9/23/2017)... -- Janssen Biotech, Inc. (Janssen) announced today that it ... Food and Drug Administration (FDA) for the Biologics License ... of moderately to severely active rheumatoid arthritis (RA). The ... to further evaluate the safety of sirukumab in the ... "We are disappointed by ...
(Date:9/22/2017)... , Sept. 22, 2017 AVACEN Medical ... device is now successfully helping those with the widespread ... Fibromyalgia diagnosed Amanda in Essex, England ... and washing my hair, experiencing no sleep at all, ... painful spasm… I cannot recommend [the AVACEN 100] enough, ...
Breaking Medicine Technology: