Navigation Links
Scientists Unravel the Mystery behind Calbindin-D28K Protein

Researcher Dr. John Cavanagh in association with fellow researchers from the Mayo Clinic and Duke University explain in detail about the shape function //and structure of the protein, calbindin-D28K.

They analyzed the protein with great accuracy and studied its interaction with the other proteins to develop drugs against neurodegenerative diseases. The researchers published the findings in the journal Nature Structural and Molecular Biology.Calbindin-D28K is a protein that either grabs calcium from areas that have too much or serves as an on/off switch for further chemical reactions. It is known for its flexibility; it is found in the kidneys, pancreas, ocular nerve and in abundant quantities in the brain. Recent studies show, Cavanagh says, that it acts as a bodyguard in the brain, binding to and inhibiting caspase-3, a protein that stimulates plaque formation and tangle formation, which are hallmark characteristics of neurodegenerative disease.

Until now, however, the structure of calbindin-D28K remained a mystery. ‘If you don't know the shape of the protein, you can't figure out how it works,’ Cavanagh says. ‘It took a long time – about five years – but we've characterized the structure of this protein and found where it binds caspase-3. Insight into how it binds to caspase-3 might lead to a way of exploiting those interactions to develop therapeutics.’ It took a long time to characterize calbindin-D28K, Cavanagh says, because it was initially a challenge to force cells to make enough protein in order to do the requisite studies. Additionally, many parts of the protein are very similar and so are extremely difficult to distinguish from each other. The research team used nuclear magnetic resonance to get a high-resolution picture of what the protein looks like. In this painstaking technique – occurring inside machines that have magnetic fields several hundred times greater than the Earth's magnetic pull – radio waves are bounced off the approx imately 5,000 nuclei in the protein.

‘When you hit a nucleus with a radiofrequency pulse, it resonates, sort of making its own little noise, like a tuning fork,’ Cavanagh says. ‘The frequency at which the nuclei resonate after being hit with a pulse is very specific to their specific position in the protein. So after we hit all of them with a pulse, it's like hitting all the keys of a piano at the same time and it's just an awful mess. And remember, we're doing this for 5,000 separate keys. Yet, we're able to untangle this mess to find the specific frequency of each nucleus and relate that to where it lies in the protein.’ Cavanagh isn't satisfied with this knowledge, however. He says the shape-shifting protein sometimes contains no calcium. When it grabs calcium, it changes its shape. ‘This could be why the protein plays so many different roles,’ Cavanagh says. ‘Proteins that change shape usually serve as on/off switches, but this protein also grabs calcium and takes it elsewhere. Now we're working to determine the structure of this protein when it has no calcium.’

Souce Eurekalert
'"/>




Related medicine news :

1. Scientists plan human cloning clinic in the United States
2. Scientists found ancient Human Germ Killer
3. Scientists locate key hormone involved in appetite control
4. Scientists open the book of life
5. Scientists review SARS
6. Scientists crack dengue fever puzzle
7. Scientists push to lower hidden sodium in food
8. Indian Scientists Make Wide-Ranging Analysis And Annotation Of X Chromosome
9. Scientists have found effective brain regions for deep brain stimulation for Parkinson’s
10. Scientists reveal the secrets of sarcasm
11. Scientists Unveil Mechanism Behind Resistance to Severe Malaria
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/9/2016)... ... February 09, 2016 , ... IsoComforter, ... the introduction of the newly designed, innovative shoulder wrap. The newly designed ... coverage for the injured arm and shoulder to promote faster healing. It is ...
(Date:2/9/2016)... ... February 09, 2016 , ... Sir ... company , is proud to announce that many of their franchises have received ... service. The hard surface restoration franchises received customer recognition through positive reviews and ...
(Date:2/9/2016)... ... February 09, 2016 , ... ... officially been won. A team from 21st Century Medicine (21CM) ( http://www.21cm.com/ ... preserve the delicate neural circuits of an intact rabbit brain for extremely long-term ...
(Date:2/8/2016)... (PRWEB) , ... February 08, 2016 , ... ... authorization with BASF Human Nutrition into the Food & Beverage and ... has been BASF’s channel partner throughout Canada and USA geographies east of the ...
(Date:2/8/2016)... ... February 08, 2016 , ... The Valentine’s Season is famous for gift giving with flowers, chocolates ... they are loved. This year, for more than 5.6 million Americans suffering with Alzheimer’s, ... be enough to remind them of the lives they’ve led and the people they’ve touched. ...
Breaking Medicine News(10 mins):
(Date:2/9/2016)... 9, 2016   AllCare Plus Pharmacy announced ... Seal of Approval ® for Home Care ... standards. The Gold Seal of Approval ® is ... to providing safe and effective care.  ... underwent a rigorous on-site survey in January 2016. The ...
(Date:2/9/2016)... Feb. 9, 2016 The new report "Global Diagnostic Ultrasound ... & Consulting group reveals that global diagnostic ultrasound devices market was ... to US$ 7,466.3 million by 2019 at a CAGR of 6.8% ... global ultrasound market has been analyzed for six geographies of ... Asia-Pacific , Latin America , ...
(Date:2/9/2016)... , Feb. 9, 2016 The global ... 2014, and it is expected to grow with a ... the glass prefilled syringes segment dominated the global prefilled ... --> --> The global market ... rate, due to increasing geriatric population, increasing demand for ...
Breaking Medicine Technology: