Navigation Links
Scientists Unravel the Mystery behind Calbindin-D28K Protein

Researcher Dr. John Cavanagh in association with fellow researchers from the Mayo Clinic and Duke University explain in detail about the shape function //and structure of the protein, calbindin-D28K.

They analyzed the protein with great accuracy and studied its interaction with the other proteins to develop drugs against neurodegenerative diseases. The researchers published the findings in the journal Nature Structural and Molecular Biology.Calbindin-D28K is a protein that either grabs calcium from areas that have too much or serves as an on/off switch for further chemical reactions. It is known for its flexibility; it is found in the kidneys, pancreas, ocular nerve and in abundant quantities in the brain. Recent studies show, Cavanagh says, that it acts as a bodyguard in the brain, binding to and inhibiting caspase-3, a protein that stimulates plaque formation and tangle formation, which are hallmark characteristics of neurodegenerative disease.

Until now, however, the structure of calbindin-D28K remained a mystery. ‘If you don't know the shape of the protein, you can't figure out how it works,’ Cavanagh says. ‘It took a long time – about five years – but we've characterized the structure of this protein and found where it binds caspase-3. Insight into how it binds to caspase-3 might lead to a way of exploiting those interactions to develop therapeutics.’ It took a long time to characterize calbindin-D28K, Cavanagh says, because it was initially a challenge to force cells to make enough protein in order to do the requisite studies. Additionally, many parts of the protein are very similar and so are extremely difficult to distinguish from each other. The research team used nuclear magnetic resonance to get a high-resolution picture of what the protein looks like. In this painstaking technique – occurring inside machines that have magnetic fields several hundred times greater than the Earth's magnetic pull – radio waves are bounced off the approx imately 5,000 nuclei in the protein.

‘When you hit a nucleus with a radiofrequency pulse, it resonates, sort of making its own little noise, like a tuning fork,’ Cavanagh says. ‘The frequency at which the nuclei resonate after being hit with a pulse is very specific to their specific position in the protein. So after we hit all of them with a pulse, it's like hitting all the keys of a piano at the same time and it's just an awful mess. And remember, we're doing this for 5,000 separate keys. Yet, we're able to untangle this mess to find the specific frequency of each nucleus and relate that to where it lies in the protein.’ Cavanagh isn't satisfied with this knowledge, however. He says the shape-shifting protein sometimes contains no calcium. When it grabs calcium, it changes its shape. ‘This could be why the protein plays so many different roles,’ Cavanagh says. ‘Proteins that change shape usually serve as on/off switches, but this protein also grabs calcium and takes it elsewhere. Now we're working to determine the structure of this protein when it has no calcium.’

Souce Eurekalert
'"/>




Related medicine news :

1. Scientists plan human cloning clinic in the United States
2. Scientists found ancient Human Germ Killer
3. Scientists locate key hormone involved in appetite control
4. Scientists open the book of life
5. Scientists review SARS
6. Scientists crack dengue fever puzzle
7. Scientists push to lower hidden sodium in food
8. Indian Scientists Make Wide-Ranging Analysis And Annotation Of X Chromosome
9. Scientists have found effective brain regions for deep brain stimulation for Parkinson’s
10. Scientists reveal the secrets of sarcasm
11. Scientists Unveil Mechanism Behind Resistance to Severe Malaria
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/23/2017)... ... February 23, 2017 , ... EBSCO ... the National Institute for Health and Care Excellence (NICE) framework. The ... National Health Service (NHS) to search, order and purchase medical and healthcare-related content ...
(Date:2/23/2017)... ... ... Dr. David Mahon leads Siena Dental, a comprehensive dental practice located at ... Top Patient Rated Henderson Dentist by Find Local Doctors earlier this year. ... dentists who have earned high ratings and superior patient reviews from multiple credible sources. ...
(Date:2/23/2017)... ... February 23, 2017 , ... ERT, ... trials, today announced that Premier Research, a leading clinical development service provider, has ... are becoming increasingly complex, due in part to an array of circumstances including ...
(Date:2/23/2017)... , ... February 23, 2017 , ... ... publication and community for those in the fight against cancer, has produced a ... their success. HRA will release top-line findings in a webinar, Defining Compassionate ...
(Date:2/23/2017)... ... (PRWEB) ... opportunity for the nation to come together to combine its ... – with its favorite fruit – apples! To celebrate National ... to join the “Apple Madness” bracket tournament – a five-week, ...
Breaking Medicine News(10 mins):
(Date:2/24/2017)... Feb 24, 2017 Research and Markets has announced ... - 2016" report to their offering. ... The latest research Dry eye Drugs Price Analysis and ... Dry eye market. The research answers the following questions: ... and their clinical attributes? How are they positioned in the Global Dry ...
(Date:2/24/2017)... 23, 2017 The U.S. Food and ... BC Kit, performed on the Pheno System. This ... cause bloodstream infections and provide information about which ... (antibiotic sensitivity). The test also reduces the amount ... information, which can guide antibiotic treatment recommendations more ...
(Date:2/24/2017)... 23, 2017  This report analyzes the worldwide markets ... Products: Intermediates, Analytical, and Others. The ... Pharmaceuticals, and Agrochemicals. The report provides separate comprehensive analytics ... Europe , and Rest of World. Annual ... through 2022. Also, a six-year historic analysis is provided ...
Breaking Medicine Technology: