Navigation Links
Salmonella Bacteria Are Deceptive

Salmonella bacteria are known as the agent involved in food poisoning and account for hundreds of thousands of deaths worldwide. // However this bacterium invades by stealth and hides from macrophages before entering them and multiplying inside them. This is ironical since macrophages are the cells meant to detect such bacteria.

Just how Salmonella escapes detection by macrophages, turning predator cells to prey complicit in promoting infection, has seemed impossibly complicated, a needle-in-a-haystack proposition involving thousands of proteins, the building blocks that carry out cells' vital functions.

Applying the high-volume sorting and analytical power of proteomics--a detailed survey of microbial proteins present in the 24 hours that follow mouse-macrophage infection--a team led by Liang Shi of the Department of Energy's Pacific Northwest National Laboratory has turned up a suspect protein.

The discovery of the protein, dubbed STM3117, is detailed today (Sept. 29) in The Journal of Biological Chemistry. Knocking out the gene that codes for STM3117, the researchers subsequently crippled the microbe's ability to multiply inside macrophages. Shi and colleagues say the protein and two closely related proteins discovered in the study are similar in genetic sequence to those known to make and modify chemicals in the microbe's cell wall called peptidoglycan.

Drug and vaccine designers armed with this mouse-model information can target chemicals or immune responses that disrupt peptidoglycan synthesis and other processes linked to Salmonella's colonization of macrophages in humans, said Joshua Adkins, a co-author on Shi's paper and lead author of a related study in Molecular & Cellular Proteomics last month. A quick identification of these proteins, Adkins added, could help physicians assess the virulence of a given strain.

The candidate proteins were winnowed from among 315 possibilities that emerged through a combination of techniques, culminating in measurements by Fourier-transform mass spectrometry, or FT-MS. A suite of FT-MS instruments customized by co-author and PNNL-based Battelle Fellow Richard D. Smith enabled the team to rapidly separate and identify many proteins at once even as macrophages were being infected.

Most of the initial candidates were designated "house-keeping" proteins, or those whose numbers relative to other proteins remained more or less constant during the course of infection. But 39 proteins shot up in number during bacterial colonization of macrophages, and of those, a handful or so--including STM3117--responded specifically to a macrophage protein associated with resistance to microbial infection. A standard assay called Western blot confirmed the abundance increases of that small group of proteins during infection.

Source-Eurekalert
RAS
'"/>




Related medicine news :

1. Public Requested To Discard Cheese Products Contaminated With Salmonella
2. Salmonella Poisoned Frozen Pasta Hit Six
3. Salmonella Affected Cases Rises to 31
4. Cadbury Blamed for Salmonella Outbreak
5. Salmonella Outbreak under Investigation
6. Tomatoes: Culprits of Salmonella outbreak
7. Salmonella enteritidis In Chicken Meat Causes Sickness
8. Salmonella Contamination Suspected In A Brand Of Fresh Spinach
9. Health Officials Suspect Salmonella In Peanut Butter
10. Consumers Warned of Using Cat Food Due to Salmonella Contamination
11. Salmonella Poisoning Suspected In Peanut Butter
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/3/2016)... Indianapolis, Ind. (PRWEB) , ... May 03, 2016 ... ... today that it has been selected by Tidelands Health, a three hospital system ... need to be constantly on the lookout for technology that enhances communication, drives ...
(Date:5/3/2016)... ... , ... Nationally recognized personal injury law firm Monge & Associates is honoring Moms ... who wants one for their Mother or any other mother figure. , According to law ... Monge goes on to say, “we are choosing to do this by giving free ...
(Date:5/3/2016)... ... 2016 , ... Ogawa World USA introduced the first of its kind ... Smart Application. The Smart 3D combines the best in technological advancement, design, and massage ... and specialized massage program, each user has a tailored experience unparalleled by any other ...
(Date:5/3/2016)... ... May 03, 2016 , ... ... Canada’s top Information and Communication Technology (ICT) companies in the annual Branham300 listing. ... companies operating in Canada, as ranked by revenue. , “We are honored ...
(Date:5/3/2016)... ... May 03, 2016 , ... Huntington Beach orthopedic ... of surgical procedure that can be used to diagnose and treat joint problems. During ... inserted. These instruments include a special lighting system and lens that illuminate and magnify ...
Breaking Medicine News(10 mins):
(Date:5/2/2016)... 2, 2016 Kalorama Information noted the 5 ... market in a recent white paper.  The healthcare market ... usage, a growing market are among the top drivers ... report EMR 2016: The Market for Electronic ... complete study of the EMR industry, and the report ...
(Date:5/2/2016)...  Deerfield announced today it led the $44.5 ... Graybug Vision is an early stage pharmaceutical company ... for ocular diseases including wet age-related macular degeneration ... developed at Johns Hopkins University and has been ... Graybug Vision is developing ophthalmology products enabling ...
(Date:5/2/2016)... LAWRENCEVILLE, N.J. , May 2, 2016 /PRNewswire/ ... drug development company, today announced data from the ... dose escalating clinical trial (the OVATION Study) combining ... of care for the treatment of newly-diagnosed patients ... chemotherapy followed by interval debulking surgery.  In the ...
Breaking Medicine Technology: