Navigation Links
Salmonella Bacteria Are Deceptive

Salmonella bacteria are known as the agent involved in food poisoning and account for hundreds of thousands of deaths worldwide. // However this bacterium invades by stealth and hides from macrophages before entering them and multiplying inside them. This is ironical since macrophages are the cells meant to detect such bacteria.

Just how Salmonella escapes detection by macrophages, turning predator cells to prey complicit in promoting infection, has seemed impossibly complicated, a needle-in-a-haystack proposition involving thousands of proteins, the building blocks that carry out cells' vital functions.

Applying the high-volume sorting and analytical power of proteomics--a detailed survey of microbial proteins present in the 24 hours that follow mouse-macrophage infection--a team led by Liang Shi of the Department of Energy's Pacific Northwest National Laboratory has turned up a suspect protein.

The discovery of the protein, dubbed STM3117, is detailed today (Sept. 29) in The Journal of Biological Chemistry. Knocking out the gene that codes for STM3117, the researchers subsequently crippled the microbe's ability to multiply inside macrophages. Shi and colleagues say the protein and two closely related proteins discovered in the study are similar in genetic sequence to those known to make and modify chemicals in the microbe's cell wall called peptidoglycan.

Drug and vaccine designers armed with this mouse-model information can target chemicals or immune responses that disrupt peptidoglycan synthesis and other processes linked to Salmonella's colonization of macrophages in humans, said Joshua Adkins, a co-author on Shi's paper and lead author of a related study in Molecular & Cellular Proteomics last month. A quick identification of these proteins, Adkins added, could help physicians assess the virulence of a given strain.

The candidate proteins were winnowed from among 315 possibilities that emerged through a combination of techniques, culminating in measurements by Fourier-transform mass spectrometry, or FT-MS. A suite of FT-MS instruments customized by co-author and PNNL-based Battelle Fellow Richard D. Smith enabled the team to rapidly separate and identify many proteins at once even as macrophages were being infected.

Most of the initial candidates were designated "house-keeping" proteins, or those whose numbers relative to other proteins remained more or less constant during the course of infection. But 39 proteins shot up in number during bacterial colonization of macrophages, and of those, a handful or so--including STM3117--responded specifically to a macrophage protein associated with resistance to microbial infection. A standard assay called Western blot confirmed the abundance increases of that small group of proteins during infection.

Source-Eurekalert
RAS
'"/>




Related medicine news :

1. Public Requested To Discard Cheese Products Contaminated With Salmonella
2. Salmonella Poisoned Frozen Pasta Hit Six
3. Salmonella Affected Cases Rises to 31
4. Cadbury Blamed for Salmonella Outbreak
5. Salmonella Outbreak under Investigation
6. Tomatoes: Culprits of Salmonella outbreak
7. Salmonella enteritidis In Chicken Meat Causes Sickness
8. Salmonella Contamination Suspected In A Brand Of Fresh Spinach
9. Health Officials Suspect Salmonella In Peanut Butter
10. Consumers Warned of Using Cat Food Due to Salmonella Contamination
11. Salmonella Poisoning Suspected In Peanut Butter
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/27/2016)... ... ... More than a third of American adults are considered obese, says the ... increased attention in recent years, as an article published May 18th on ... people are familiar with the basic requirements of maintaining a healthy diet and exercise ...
(Date:5/27/2016)... ... ... With over 60 percent of acute stroke survivors being left unable to ... in the rehabilitation process has steadily increased. Ekso Bionics had been working to help ... stroke. , Ekso Bionics has now received clearance from the U.S. Food and Drug ...
(Date:5/27/2016)... ... May 27, 2016 , ... ... is bolstered by inspiring human interest stories, courtesy of leaders in the nursing ... tech within the industry, from leading advocates and associations—namely Jones & Bartlett Learning. ...
(Date:5/26/2016)... ... 2016 , ... There are many ways to cook a hot dog, but ... Americans prefer their dogs straight off the grill. Of the 90 percent of Americans ... to cook a hot dog, far outpacing other cooking methods such as steaming (12 ...
(Date:5/26/2016)... ... ... Connor Sports, through its Connor Cares initiative, will continue to expand ... Tour that will commemorate the Indiana Fever legend’s hall-of-fame career and final WNBA ... levels of the game, Connor Sports has committed to a significantly increased focus on ...
Breaking Medicine News(10 mins):
(Date:5/27/2016)... -- The healthcare sector is large and ... falling under its umbrella.  A rather overlooked sector are ... about, these healthcare companies are still trying to prove ... by far the largest consumer of the healthcare market ... Nutranomics Inc. (OTC: NNRX), KollagenX Corp. (OTCQB: KGNX), Bioelectronics ...
(Date:5/26/2016)... , May 26, 2016 Since ... matured into an essential life science tool for conducting ... applications. BCC Research reveals in its new report that ... growth phase, one powered by a range of new ...      (Logo: http://photos.prnewswire.com/prnh/20140723/694805 ) , ...
(Date:5/25/2016)... , May 25, 2016  Zymo Research Corp. ... their new reference materials that help researchers obtain ... collection to analyses. The rapid growth of the ... researchers to have standard methods to improve the ... Biases inherently exist at every step of the ...
Breaking Medicine Technology: