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New Technique To Diagnose Cancer At Molecular Level

tics Institute, said that scientists know that cancer tissue has a unique molecular fingerprint that can distinguish it from healthy tissue. But attempts to target cells via these fingerprints have largely proved futile because there are few molecular tools to recognize the fingerprints. The UF team sought to create these tools in the form of aptamers, or short strands of chemically synthesized DNA. These aptamers exploit the differences on the surface of cells to discern cancerous ones. Key to the approach is it does not require prior knowledge of cancer indicators, Tan said. "Using the cell-based aptamer selection strategy, we can generate aptamers which can specifically recognize any kind of cells without prior knowledge of molecular changes associated with the disease," he said. In experiments, the researchers showed they could successfully design sets of aptamers that would recognize leukemia cells that had been mixed in with normal bone marrow cells. The aptamers also successfully distinguished leukemia T-cells from lymphoma B-cells. Both results indicate that the aptamer method could be used to identify many different types of cancer, researchers said. Clinicians using such molecular probes should be able to "find cancer in a much earlier stage when the tumors are much smaller," enabling doctors to begin treatment earlier, Li said. Richard Zare, a professor and chairman of the Stanford University department of chemistry, said he is "hugely impressed" by the findings reported in the PNAS paper. "It represents a most clever, new approach to using the differences at the molecular level between any two types of cells for the identification of molecular signatures on the surface of targeted cells," he said. "I can easily imagine that it will have a most significant impact on developing therapies for disease states." Contact: Weihong Tan tan@chem.ufl.edu 352-846-2410 University of Florida Source: Eurekalert
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