Navigation Links
New Multi-functional Sensing Tool To Investigate Cystic Fibrosis

A research team from Georgia Institute of Technology has developed a new multi-functional sensing system to investigate adenosine triposphate (ATP) release and its role in cystic fibrosis. //

This patented technology adds recessed micro- and nano-electrodes to the tip of an atomic force microscope (AFM), creating a single tool that can simultaneously monitor topography along with electrochemical activity at the cell surface.

Researchers will present information on the research on March 26 at the American Chemical Society’s 231st meeting in Atlanta during a session on new approaches in analytical chemistry.

The new multi-functional imaging technique will advance the study of biological samples, said Boris Mizaikoff, an associate professor at Georgia Tech’s School of Chemistry and Biochemistry and director of its Applied Sensors Lab.

“Conventional AFM can image surfaces, but usually provides limited chemical information,” he explained. “And though scanning electrochemical microscopy (SECM), another probing technique, provides laterally resolved electrochemical data, it has limited spatial resolution. By combining AFM and SECM functionality into a single scanning probe, our tool provides researchers with a more holistic view of activities at the cell surface.”

In addition to Mizaikoff and Kranz, the team also includes post-doctoral scholar Jean-Francois Masson and graduate student Justyna Wiedemair.

In the ATP study, which is sponsored by the National Institutes of Health and done in collaboration with Douglas Eaton at Emory University’s School of Physiology, the Georgia Tech team used the multi-scanning biosensors to study ATP release at the surface of live epithelial cells (cells that cover most glands and organs in the body). ATP, a chemical involved in energy transport, is of interest to medical researchers because elevated levels have been linked with cystic fibrosis, a disease that affects one out of every 2,500 people in the United States.

Using epithelial cell cultures from Emory, the Georgia Tech researchers have demonstrated that their multi-functional biosensors work at the live-cell surface during in vitro studies.

“Before you can identify what triggers the ATP release, we must be able to quantitatively measure the released species at the cell surface,” Mizaikoff said, noting that many pathological events involve the disruption of chemical communication and molecular signaling between cells, especially in the nervous system, lungs and kidneys.

Improved understanding of cellular communication can lead to new strategies for treating diseases, Mizaikoff added: “Being able to operate sensors in an electrochemical imaging mode at the micro- and nanoscale is an exciting opportunity for complementing optical imaging techniques. There are many clinical research problems that these biosensors can help with.”

During the same ACS session, the Georgia Tech team will also present findings of a related project.

A collaboration with Estelle Gauda at Johns Hopkins University and also supported by NIH grants, this project monitors ATP release at the carotid body. (The carotid body is a chemoreceptor that, among other functions, monitors oxygen content in the blood and helps control respiration.)

Chronic oxygen stress – too much or too little oxygen during early postnatal development – can lead to a deficiency in the amount of oxygen reaching body tissues in premature infants and newborn animals. But little is known about how oxygen stress affects regulatory networks and alters chemoreceptors. To gain insights, the Georgia Tech researchers will study ATP, which is among the signaling molecules released by the carotid body.

Researchers incorporate the same technology used for the multi-functional scanning probe. For this study, however, they have tailored the biosensor to work at a larger scale – microelectrodes are about 25 micrometers in diameter as opposed to the sub-micrometer dimensions of the combined AFM-SECM approach.

“There are a lot of emerging sensor technologies, but few have been adapted for routine use in medical research, which is one of the development goals at the Applied Sensors Lab,” Mizaikoff said. “As analytical chemists, we want to develop quantitative sensing devices that can answer important questions for clinical researchers.”

ource: Newswise

Related medicine news :

1. Sensing The Health Hazard MP3 Makers Tone Down The Volume
2. Sensing Devices to Measure Sleep In the Pipeline
3. Stem Cells In Brain Have Potential For Sensing Damage And To Repair
4. S.Africa Sets Up Task Team To Investigate Anthrax Outbreak In KNP
5. MMR- Autism- Bowel Problems Connection To Be Investigated
6. Researchers Investigate Protein that Protects Tumors
7. European Regulators Investigate Common Painkiller Drugs
8. Researchers Investigate Molecular Basis of Memory
9. Researchers Investigate Gene Linked With Circadian Liver Clock
10. Potential for Adult Stem Cells to Repair Hearts Damaged Investigated
11. CDA Investigates Case of Bovine Tuberculosis
Post Your Comments:

(Date:11/30/2015)... ... November 30, 2015 , ... The world of hair ... and follicular unit extraction. These techniques and procedures have been in use for many ... hair loss. While Dr. Parsa Mohebi, M.D. has utilized many of these methods over ...
(Date:11/30/2015)... ... , ... The presidential race normally deals with political issues of national importance ... news story when Donald Trump makes disparaging remarks about Hillary Clinton’s hairstyle? It is ... wants to admit when it comes to how people are viewed by others. , ...
(Date:11/30/2015)... Los Angeles, CA (PRWEB) , ... November 30, 2015 , ... ... hunger cravings that drive people to overeat are not necessarily caused by real hunger, ... brain that the stomach needs food. He notes that, while many patients are aware ...
(Date:11/30/2015)... ... November 30, 2015 , ... RoamRight, a leading provider of ... Public Television’s Travel With Kids to promote family vacations around the world. ... they explore international destinations and educate families about the people and places of the ...
(Date:11/30/2015)... ... November 30, 2015 , ... At Grand Dental PC, ... convenient setting. , When you have dental problems, you need to turn to a ... effectively diagnose and treat your needs, a friendly dentist who counsels you on the ...
Breaking Medicine News(10 mins):
(Date:11/30/2015)... , November 30, 2015 --> ... report "Dental Lasers Market by Product (Soft Tissue, All Tissue, ... User (Hospitals, Clinics), and Geography - Global Forecast to 2020", ... by 2020, at a CAGR of 5.2% during the forecast ... market data Tables and 62 Figures spread through 167 P ...
(Date:11/30/2015)... Oramed Pharmaceuticals Inc. (NASDAQ: ORMP ), a clinical-stage ... delivery systems, announced today it has signed definitive licensing ... of Technologies Co., Ltd. ("HTIT") for exclusive rights to ... China , Hong Kong ... signed at the Israel Knesset (Parliament). ...
(Date:11/30/2015)... , Nov. 30, 2015   Nuance Communications, ... The National Decision Support Company (NDSC) today jointly ... and collaboration capabilities that utilize the American College of ... healthcare provider organizations to comply with current and ... --> --> By combining clinical decision ...
Breaking Medicine Technology: