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New Approach Assesses Risk of Water-Borne Pathogen Disease

and soil sources are time-consuming, laborious, and usually unable to identify pathogenic Leptospira at all. To overcome these limitations, the scientists analyzed relatively small quantities of surface water – for instance water from gutters, wells, puddles and streams – using quantitative real-time PCR assays that amplify small amounts of DNA. By measuring DNA, the researchers were able to effectively describe and quantify the amount of pathogenic bacteria present in the water samples.

The scientists then were able to connect the molecular identification of the bacterial pathogens in the water samples to those actually infecting people and causing acute leptospirosis in rural and urban areas. This approach allowed for a precise mechanistic connection between source of infection and human disease, allowing the researchers to measure the environmental risk for bacterial infection – an approach never used before.

“This study is important because we have connected clinical and basic science to provide a quantitative risk assessment for water-borne diseases,” said Vinetz. “This method of risk assessment for infection may also be applicable to other water-borne diseases such as those caused by Shigella, Salmonella, Cryptosporidium, and E. coli.”

(Sorce: Newswise)
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