Scientists at the University of Southern California have applied a nanoscale imaging method to a biological system, helping to clear up an old puzzle of the global carbon and nitrogen cycle . Their study appears as an advanced online publication of The ISME Journal.
A state of the art NanoSIMS instrument (short for nanometer-scale, secondary ion mass spectrometry) located at Lawrence Livermore National Laboratory can image and measure minute amounts of chemical elements.
Douglas Capone and Kenneth Nealson, USC Wrigley Professors of Environmental Biology and Geology, respectively, used NanoSIMS to track the flow of carbon and nitrogen inside two types of cells in the freshwater organism Anabaena oscillarioides.
This instrument is allowing us to look at how these two elements are being taken up and are being transported around these different types of cells, Capone said. Its basically allowing us to image elements at very high resolution.
The organism fixes, or pulls from the atmosphere, both carbon and nitrogen. For decades biologists wondered how the organism could fix both elements, since carbon fixation associated with photosynthesis produces oxygen, while nitrogen fixing needs an oxygen-free environment.
Previous studies have shown that some photosynthetic cells can differentiate into heterocysts: thick-walled relatives that fix nitrogen but do not produce oxygen.
Using NanoSIMS, Capone and Nealson followed nitrogen as it is fixed in heterocysts and then transported to the other cells, where it is needed as a nutrient.
Capone and Nealson also were able to observe cellular differentiation: as the single-stranded organism grows, the nitrogen concentration in the cell halfway between two existing heterocysts falls below a threshold. Its basically allowing us to image elements at very high resolution.
The drop in nitrogen starts a process that turns the cell into a hePage: 1 2 Related medicine news :1
. Identifying bacteria made amiable2
. Identifying brain lesions with impaired consciousness3
. Identifying Virus Found To Be Essential for HIV Patients4
. Identifying Those At Risk For Diabetes5
. Identifying Predictors Of Anthrax6
. Identifying The Risks Of Developing Schizophrenia7
. Identifying Irregular Heart Beats8
. Identifying Those At Risk For Diabetes9
. Identifying Heart Disease Early10
. Identifying The Risk For Kidney Infection 11
. Identifying What Consumers Look For On Food Stuff Labels