Navigation Links
Mouse Model Demonstrates Immune System Reaction In Chlamydia Infection

Harvard Medical School (HMS) researchers have mapped out the way immune system in mouse models responds to germs in the genital tract //during the initial stage of Chlamydia trachomatis infection. The study is published in the July 24 early edition online of the Proceedings of the National Academy of Sciences and could prove important in the development of vaccine against the disease.

"Right now Chlamydia is sensitive to treatment with antibiotics, but the problem is that many people have 'silent' infections that remain untreated," said researcher Michael Starnbach, PhD, HMS associate professor of microbiology and molecular genetics. "These undiagnosed infections over time lead to complications like tubal pregnancy and infertility. The goal would be to vaccinate young people to keep them from suffering from undiagnosed infection and the bad outcomes associated with it."

Most pathogens (disease-causing bacteria or viruses) enter a host by penetrating mucosal surfaces such as the lung, intestine, or genito-urinary tract. The prevalence of sexually transmitted diseases has prompted studies to understand how infection is established in the genital tract and how pathogens are cleared from this site. Before Starnbach's study, however, it had not been possible to monitor invader-specific T cell (fighter cell) responses to initial infection in reproductive tissue, despite the recognized importance of T cells in controlling a number of genital pathogens

"Humans and mice have an enormous variety of T cells that are prepared to respond to pathogens – even pathogens to which they have never been exposed," Starnbach said. "Yet prior to infection, the number of T cells specific for any single pathogen is extremely low. The number is so low that it is impossible to track and monitor the activity of these T cells during their first encounter with the microbe."

To circumvent the problem, Starnbach and colleagues in the HMS Department of Microbiology and Molecular Genetics identified one of the Chlamydia proteins recognized by T cells during infection and engineered mice where all the T cells in the mice exclusively respond to this Chlamydia protein. These mice are known as T cell receptor transgenic (TCRtg) mice and have none of the T cell diversity found in a normal mouse. Starnbach and his team harvested T cells from the Chlamydia-specific TCRtg mice, labeled them with a dye, and injected them into normal mice. By boosting the number of Chlamydia-specific T cells in the recipient mice, they were able to identify and monitor them as they responded to infection.

"We found that when the recipient mice were infected with Chlamydia, the labeled T cells became activated specifically in the lymph nodes near the reproductive tract, expanded in number in those lymph nodes, and migrated into the mucosa lining the genital tract," Starnbach said. "We also found that the T cells recruited to the genital mucosa secrete gamma interferon as they respond to Chlamydia infection. Gamma interferon secretion has been described as the key molecule T cells use to rid the body of Chlamydia."

The findings show how T cell responses can be studied in reproductive tissues, which is likely to reveal avenues to the development of vaccines against Chlamydia, and possibly other STDs.

"In this report, we identify one candidate protein, Cta1, and show that T cells specific for Cta1 can reduce Chlamydia infection," Starnbach said. "In designing a vaccine, we would want to make sure the vaccine stimulates T cells with characteristics that cause them to home to the genital tract - the site of infection. We also would want them to respond with the same arsenal of protective factors – such as the secretion of gamma interferon that we show occurs using our TCRtg tools."

The goal of vaccines is to stimulate a response that mimics exposure to a pathogen, without the risks of actual infecti on. When a vaccine successfully accomplishes this, protection against future infection results.

Contact: Judith Montminy

public_affairs@hms.harvard.edu

617-432-0442

Harvard Medical School

Source: Eurekalert
'"/>




Related medicine news :

1. Mouse Allergen Found In Homes Could Trigger Asthma In Kids
2. Researchers Develop Mouse Model Of Brain Tumor
3. German Scientists Create Stem Cells From Mouse Testes
4. Mouse Models To Determine The Cause Of Aggressive Leukemia
5. Mouse Model Beneficial to Study of Head and Neck Cancer
6. Scientists Discover New RNAs in Mouse Testicles
7. Scientists Isolate Leukemia Stem Cells in Mouse Model
8. Mouse Gene With Gene Stutter Will Help Leukemia Research
9. Rett Syndrome Reversed in Genetic Mouse Model
10. Treatment Extends Survival in Mouse Model of Spinal Muscular Atrophy
11. Mouse Study Suggests Anxiety Disorders Take Root in Infancy
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/10/2016)... ... February 09, 2016 , ... A new leadership team for Mid-South ... President Joe Wiley made the announcement Monday night, Feb. 8, prior to the evening ... of FHU and the creator of GO! Camp, has been named director. Gayle McDonald, ...
(Date:2/10/2016)... (PRWEB) , ... February 09, 2016 , ... ... at Clevens Face and Body Specialists are delighted to welcome a new addition ... joins Clevens Face and Body Specialists as a nurse practitioner performing cosmetic ...
(Date:2/10/2016)... ... February 09, 2016 , ... Traumatic ... cause of injury may be one of many possible sources: sports, car accidents, ... Education Course , Mastering Rehab Solutions for the Complexities of Concussions ...
(Date:2/9/2016)... Florida (PRWEB) , ... February 09, 2016 , ... Steven ... the Taste of South Florida Event, an upscale fundraiser held in South Florida. The ... sponsors and guests alike. This year the event will be held in a new, ...
(Date:2/9/2016)... ... February 09, 2016 , ... Interstate Restoration LLC, a full ... today the acquisition of Hawaii DKI. Hawaii DKI is one of the more ... companies who excel at service and response helps support our goal of becoming ...
Breaking Medicine News(10 mins):
(Date:2/9/2016)... Blood Monitoring & Cardiac Monitoring Devices Market Assessment & Forecast: 2015-2019" ... market for blood monitoring & cardiac monitoring devices was valued at ... 24,830.1 million by 2019 at a CAGR of 6.8% from 2015 ... Europe , Asia-Pacific , Latin ... Africa . The three major factors determining growth of the ...
(Date:2/9/2016)... , Feb. 9, 2016  Increasingly, health care ... their vital signs with wireless technology. With the Vios ... automate patient oversight and remotely detect problems before they ... signs across in-hospital environments. the United ... United States . --> The ...
(Date:2/9/2016)... , Feb. 9, 2016  Johnson & Johnson (NYSE: ... Markets Global Healthcare Conference on Tuesday, Feb. 23, at ... Dominic Caruso , Vice President, Finance & Chief Financial ... will represent the Company in a session scheduled at ... --> www.investor.jnj.com . --> This ...
Breaking Medicine Technology: