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Master Switches Found for Adult Blood Stem Cells

with more than 1,200 of the larger variety RNA already known to be important for stem-cell maturation.

Georgantas and Civin currently are testing whether these pair predictions are valid by using a non-reproducing virus to insert genetic instructions for each of the 33 microRNAs into adult stem cells. They'll then be cultured in Petri dishes. MicroRNA-155 - the first microRNA tested - was predicted to stop stem cells from developing into red and white blood cells.

As expected, stem cells without microRNA-155 matured: they formed approximately 75 red and 150 white blood cell colonies per dish. Stem cells with microRNA-155 matured into far fewer red and white cell colonies - about seven and 30 per dish, respectively.

"Using microRNAs to stall an adult blood stem cell in its early stage could help us grow new ones in test tubes, and perhaps give us more insight into stem-cell maturation for other tissue types," says Civin.

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