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Genome Annotation by Mutation

the order and location of base pairs determined in detail scientists can identify locations in individual mice where the jumping genes have carried pieces of loxP DNA. Then, they can select a mouse with loxP on both sides of the large piece of DNA they want to mutate and study.

If the jumping gene carrying loxP hops into the middle of a gene, then the gene will be mutated. So while the method began as a way to delete large stretches of non-gene DNA, it has the bonus of also being able to disable genes more easily and inexpensively than existing knockout techniques, Wu says.


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