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Experimental Flu Vaccine Appears Promising in Early Tests

An influenza vaccine produced with the use of insect cells appeared safe and produced an immunogenic response// in healthy adults. The new research promises to be an alternative to using embryonated eggs for the development of influenza vaccine, according to a preliminary study.

"All currently licensed influenza vaccines in the United States are produced in embryonated hen's eggs. There are several well-recognized disadvantages to the use of eggs as the substrate [the base on which an organism lives or grows] for influenza vaccine. Eggs require specialized manufacturing facilities and could be difficult to scale up rapidly in response to an emerging need such as a pandemic," the authors write. They add that development of alternative substrates for influenza vaccine production has been identified as a high-priority. One potential alternative is use of the influenza virus hemagglutinin (HA; an antibody that causes red blood cells to clump together) using recombinant (genetic recombination) DNA techniques.

John J. Treanor, M.D., of the University of Rochester, N.Y., and colleagues evaluated an experimental influenza vaccine consisting of recombinant HA expressed in insect cells by a recombinant baculovirus (rHA0). The clinical trial was conducted at three U.S. academic medical centers during the 2004-2005 influenza season and included 460 healthy adults. Participants were randomly assigned to receive a single injection of saline placebo (n = 154); 75 μg of an rHA0 vaccine containing 15 μg of hemagglutinin from influenza A/New Caledonia/20/99(H1N1) and influenza B/Jiangsu/10/03 virus and 45 μg of hemagglutinin from influenza A/Wyoming/3/03 (H3N2) virus (n = 153); or 135 μg of rHA0 containing 45 μg of hemagglutinin each from all 3 components (n = 153). Serum samples were taken before and 30 days following immunization.

The researchers found: "We have shown that the rHA0 vaccine is well tolerated in healthy adults and immunogenic at
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