Navigation Links
Detecting DNA Damage With In-Vivo Alkaline Comet Assay

The comet assay has gained rapid importance in detecting various forms of DNA damage and repair.// The conventional methods have numerous disadvantages and are time consuming, resource intensive and require a large proliferating cell population.

The comet assay attracts adherents by it’s simplicity, speed, versatility and economy and is an ideal alternative supplementary in-vivo study. The Comet assay can be used to detect various types of DNA damage induced by different classes of genotoxic compounds.

The principle is that strand breakage of the duplex DNA leads to difference in size of the molecule and these fragments can be stretched out by electrophoresis. The image obtained consists of a head made up of intact DNA and a tail, which represents the fragmented DNA. Under alkaline conditions, unwinding of the double strand takes place, which enables the visualization of single strand breaks and alkali labile sites.

The main requirement of the assay is a single cell or nuclei suspension, which can be obtained from the tissue without adding to the DNA damage by the applied isolation technique. However, the assay only measures DNA damage and it remains unclear if mutations will result or whether the detected damage will be repaired before the next cell division.

There is a positive movement towards formal regulatory acceptance of the assay, which has resulted in increasing demand for the in vivo Comet assay. In response to this demand, RCC Cytotest Cell Research has established and validated protocols for different cell types.

In order to make a comparison with an acknowledged test system, the Comet assay experiments were run in parallel with an in vivo UDS test on the same hepatocyte suspensions. The results have shown that the in vivo Comet assay is a sensitive assay to detect DNA damage in the tested organs and have demonstrated the major advantage namely, the simultaneous assay of several different tissues f rom the same animal.

In conclusion, a further fully validated genotoxicity assay has been added to the battery of genotoxicity tests available at RCC. Refinement of the technique will continue as experience is accumulated.
'"/>




Related medicine news :

1. Detecting anomalies in heart rythm
2. Detecting brain aneurysms
3. Detecting Colorectal Cancer
4. Photographs An Effective Tool In Detecting Skin Cancer
5. Mammograms The Best In Detecting Breast Cancer
6. Detecting Lung Cancer Early
7. Detecting Lung Cancer In Its Initial Stages
8. A New Way Of Detecting Osteoporosis
9. CT Pulmonary Angiography Scan More Effective In Detecting Dangerous Blood Clots In The Legs
10. Researchers Develop Device For Detecting Atherosclerotic Plaques
11. India Needs To Pay More Attention To Detecting Cancer
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/2/2016)... ... December 02, 2016 , ... Center for Autism and Related ... those affected by autism spectrum disorder (ASD) and other developmental disabilities. The group, which ... give parents and other caregivers the opportunity to share stories and advice, seek help, ...
(Date:12/2/2016)... ... December 02, 2016 , ... ... of two ostomy patients, standing as living proof that attitude and determination can ... diseases and issues that spike around the holidays. This campaign will offer patients ...
(Date:12/2/2016)... ... December 02, 2016 , ... CloudLIMS.com, a class-leading provider of ... Lite. CloudLIMS Lite helps biobanks, clinical, research and testing laboratories keep track of ... new version is a faster and a more efficient product, allowing batch processing ...
(Date:12/2/2016)... ... December 02, 2016 , ... Rijuven Corp launches rejiva ( http://www.rejiva.com ... and night. No other wearable health technology on the market can deliver all that ... poeple more meaningful insights about their health than the usual heart rate and steps ...
(Date:12/2/2016)... ... December 02, 2016 , ... ... needy individuals and families from eight different sites throughout Miami-Dade and Broward counties. ... 1,000 volunteers worked very hard on Thanksgiving morning by putting together individual meals ...
Breaking Medicine News(10 mins):
(Date:12/2/2016)... YORK , December 2, 2016 ... Research titled "Global Market Study on Automated Endoscope Reprocessors: Single ... a CAGR of 8.6% Between 2016 and 2024 " the global automated ... and is expected to expand at a CAGR of 7.2% ... valuation of US$ 1,367.6 Mn by 2024. ...
(Date:12/2/2016)... , Dec. 2, 2016 In the ... sources apart from those derived from C. sativa, the ... Universita` di Napoli Federico II , the Universita` ... the first comprehensive, critical, integrated and unified inventory of ... article focuses on the remarkable chemical and structural ...
(Date:12/2/2016)... British Columbia , December 2, 2016 bioLytical ... den INSTI HIV-Selbsttest, bei den Mitgliedern des Apothekenbundes von Kenia eingeführt. ... ... INSTI HIV Self Test! (PRNewsFoto/bioLytical Laboratories) ...      (Photo: http://photos.prnewswire.com/prnh/20161201/444905 ) ...
Breaking Medicine Technology: