Navigation Links
Detecting DNA Damage With In-Vivo Alkaline Comet Assay

The comet assay has gained rapid importance in detecting various forms of DNA damage and repair.// The conventional methods have numerous disadvantages and are time consuming, resource intensive and require a large proliferating cell population.

The comet assay attracts adherents by it’s simplicity, speed, versatility and economy and is an ideal alternative supplementary in-vivo study. The Comet assay can be used to detect various types of DNA damage induced by different classes of genotoxic compounds.

The principle is that strand breakage of the duplex DNA leads to difference in size of the molecule and these fragments can be stretched out by electrophoresis. The image obtained consists of a head made up of intact DNA and a tail, which represents the fragmented DNA. Under alkaline conditions, unwinding of the double strand takes place, which enables the visualization of single strand breaks and alkali labile sites.

The main requirement of the assay is a single cell or nuclei suspension, which can be obtained from the tissue without adding to the DNA damage by the applied isolation technique. However, the assay only measures DNA damage and it remains unclear if mutations will result or whether the detected damage will be repaired before the next cell division.

There is a positive movement towards formal regulatory acceptance of the assay, which has resulted in increasing demand for the in vivo Comet assay. In response to this demand, RCC Cytotest Cell Research has established and validated protocols for different cell types.

In order to make a comparison with an acknowledged test system, the Comet assay experiments were run in parallel with an in vivo UDS test on the same hepatocyte suspensions. The results have shown that the in vivo Comet assay is a sensitive assay to detect DNA damage in the tested organs and have demonstrated the major advantage namely, the simultaneous assay of several different tissues f rom the same animal.

In conclusion, a further fully validated genotoxicity assay has been added to the battery of genotoxicity tests available at RCC. Refinement of the technique will continue as experience is accumulated.

Related medicine news :

1. Detecting anomalies in heart rythm
2. Detecting brain aneurysms
3. Detecting Colorectal Cancer
4. Photographs An Effective Tool In Detecting Skin Cancer
5. Mammograms The Best In Detecting Breast Cancer
6. Detecting Lung Cancer Early
7. Detecting Lung Cancer In Its Initial Stages
8. A New Way Of Detecting Osteoporosis
9. CT Pulmonary Angiography Scan More Effective In Detecting Dangerous Blood Clots In The Legs
10. Researchers Develop Device For Detecting Atherosclerotic Plaques
11. India Needs To Pay More Attention To Detecting Cancer
Post Your Comments:

(Date:6/24/2016)... CA (PRWEB) , ... June 24, 2016 , ... Comfort ... the American Cancer Society and the Road To Recovery® program to drive cancer patients ... seniors and other adults to ensure the highest quality of life and ongoing independence. ...
(Date:6/24/2016)... , ... June 24, 2016 , ... People across the ... Genome magazine’s Code Talker Award, an essay contest in which patients and their families ... to be presented at the 2016 National Society of Genetic Counselors (NSGC) Annual Education ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... ... Living, is proud to recognize Dr. Barry M. Weintraub as a prominent plastic ... most beautiful women in the world, and the most handsome men, look naturally ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... ... is actively feeding the Frederick area economy by obtaining investment capital for emerging ... the past 2½ years that have already resulted in more than a million ...
(Date:6/24/2016)... ... ... Venture Construction Group (VCG) sponsors Luke’s Wings 5th Annual ... Country Club at 1201 Rockville Pike, Rockville, Maryland, 20852. The event raised funds ... been wounded in battle and their families. Venture Construction Group is a 2016 Silver ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016  MedSource announced today ... its e-clinical software solution of choice.  This latest ... possible value to their clients by offering a ... preferred relationship establishes nowEDC as the EDC platform ... MedSource,s full-service clients.  "nowEDC has long been a ...
(Date:6/23/2016)... , June 23, 2016 ... "Pharmaceutical Excipients Market by Type (Organic Chemical (Sugar, ... Formulation (Oral, Topical, Coating, Parenteral) - Global Forecast to ... The global pharmaceutical excipients market ... at a CAGR of 6.1% in the forecast period ...
(Date:6/23/2016)... 2016 Research and Markets has announced ... Analysis 2016 - Forecast to 2022" report to their ... contains up to date financial data derived from varied research ... trends with potential impact on the market during the next ... which comprises of sub markets, regional and country level analysis. ...
Breaking Medicine Technology: