Navigation Links
Detecting DNA Damage With In-Vivo Alkaline Comet Assay

The comet assay has gained rapid importance in detecting various forms of DNA damage and repair.// The conventional methods have numerous disadvantages and are time consuming, resource intensive and require a large proliferating cell population.

The comet assay attracts adherents by it’s simplicity, speed, versatility and economy and is an ideal alternative supplementary in-vivo study. The Comet assay can be used to detect various types of DNA damage induced by different classes of genotoxic compounds.

The principle is that strand breakage of the duplex DNA leads to difference in size of the molecule and these fragments can be stretched out by electrophoresis. The image obtained consists of a head made up of intact DNA and a tail, which represents the fragmented DNA. Under alkaline conditions, unwinding of the double strand takes place, which enables the visualization of single strand breaks and alkali labile sites.

The main requirement of the assay is a single cell or nuclei suspension, which can be obtained from the tissue without adding to the DNA damage by the applied isolation technique. However, the assay only measures DNA damage and it remains unclear if mutations will result or whether the detected damage will be repaired before the next cell division.

There is a positive movement towards formal regulatory acceptance of the assay, which has resulted in increasing demand for the in vivo Comet assay. In response to this demand, RCC Cytotest Cell Research has established and validated protocols for different cell types.

In order to make a comparison with an acknowledged test system, the Comet assay experiments were run in parallel with an in vivo UDS test on the same hepatocyte suspensions. The results have shown that the in vivo Comet assay is a sensitive assay to detect DNA damage in the tested organs and have demonstrated the major advantage namely, the simultaneous assay of several different tissues f rom the same animal.

In conclusion, a further fully validated genotoxicity assay has been added to the battery of genotoxicity tests available at RCC. Refinement of the technique will continue as experience is accumulated.
'"/>




Related medicine news :

1. Detecting anomalies in heart rythm
2. Detecting brain aneurysms
3. Detecting Colorectal Cancer
4. Photographs An Effective Tool In Detecting Skin Cancer
5. Mammograms The Best In Detecting Breast Cancer
6. Detecting Lung Cancer Early
7. Detecting Lung Cancer In Its Initial Stages
8. A New Way Of Detecting Osteoporosis
9. CT Pulmonary Angiography Scan More Effective In Detecting Dangerous Blood Clots In The Legs
10. Researchers Develop Device For Detecting Atherosclerotic Plaques
11. India Needs To Pay More Attention To Detecting Cancer
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/27/2016)... York, NY (PRWEB) , ... May 27, 2016 , ... ... needing intervention to walk, the demand for a sustainable product to aid in the ... void and aid in the recovery of individuals with hemiplegia due to stroke. , ...
(Date:5/27/2016)... ... May 27, 2016 , ... Beleza Medspa has initiated a ... This is the first time that Coolsculpting is being used for for more ... months to ensure they meet the prescribed body-fat standard, measured by the circumference-based tape ...
(Date:5/27/2016)... (PRWEB) , ... May 27, 2016 , ... ... trusted name in the pharmaceutical, medical and food industries. Aside from its GMP ... certification (SIC), providing proof of successfully certified products, services and staff. , Validation ...
(Date:5/26/2016)... Oakland, California (PRWEB) , ... May 26, 2016 ... ... liabilities associated with discovery of thousands of defective respirators, according to court documents ... in the case of William and Becky Tyler v. American Optical Corporation, Case ...
(Date:5/26/2016)... ... ... On Memorial Day, Hope For Heroes and USA Medical Card will ... country. The nonprofit Hope For Heroes partnered with the leading provider of free ... military veterans, as well as police, firemen, and EMS professionals across the country, and ...
Breaking Medicine News(10 mins):
(Date:5/25/2016)... PUNE, India , May 25, 2016 ... "Medical Animation Market by Type (3D, 2D, 4D), by ... Patient Education), by End User (Medical Device Manufacturers, Hospitals/ ... report studies the global Medical Animation Market for the ... expected to reach USD 301.3 Million by 2021 from ...
(Date:5/25/2016)... , May 25, 2016  Zymo Research ... for their new reference materials that help researchers ... sample collection to analyses. The rapid growth of ... for researchers to have standard methods to improve ... generated. Biases inherently exist at every step of ...
(Date:5/25/2016)... SPRINGS, Florida , May 25, 2016 ... Business Conference & Expo earlier this month, the numbers ... As revenues continue to climb into the billions, more ... the newly released 4th Edition State of Legal Marijuana ... Frontier, a cannabis-focused data-analysis firm, much of the increase ...
Breaking Medicine Technology: