Navigation Links
DNA-based Detection of Heavy Metals

Researchers at the University of Illinois at Urbana-Champaign have developed a simple, disposable sensor for detecting hazardous uranium ions,// with sensitivity that rivals the performance of much more sophisticated laboratory instruments.

The sensor provides a fast, on-site test for assessing uranium contamination in the environment, and the effectiveness of remediation strategies, said Yi Lu, a chemistry professor at Illinois and senior author of a paper accepted for publication in the Proceedings of the National Academy of Sciences, and posted on its Web site.

While most DNA is double stranded, the catalytic DNA Lu's research group uses has a single strand region that can wrap around like a protein. In that single strand, the researchers fashion a specific binding site -- a kind of pocket that can only accommodate the metal ion of choice.

In this case, the researchers chose to detect uranyl, the most soluble species of uranium ion and the one that poses the greatest threat to human life.

To search for the unique sequence of DNA that could distinguish uranyl from other metal ions, the researchers used a combinatorial approach called in vitro selection. Simple and cost-effective, the selection process can sample a very large pool of DNA (up to 1,000 trillion molecules), amplify the desired sequence by the polymerase chain reaction, and introduce mutations to improve performance.

Lu, with collaborators at Illinois, the Construction Engineering Research Laboratory, Oregon State University and Oak Ridge National Laboratory, assembled the uranium sensor and tested it on soils containing varying amounts of uranium.

The presence of uranyl causes catalytic cleavage of the DNA and release of the fluorophore, resulting in a dramatic increase of fluorescence intensity. With a detection sensitivity of 11 parts per trillion, the disposable sensor rivaled the performance of much more sophisticated laboratory instruments.

"This latest success demonstrates that our methodology can be used to make cost-effective sensors for other hazardous metals, as well, with extremely high sensitivity and selectivity," Lu said. "We can also construct sensor arrays that detect and quantify many metal ions simultaneously."



Source-Bio-Bio Technology
'"/>




Related medicine news :

1. Detection of Cancer
2. Early Detection Of Prostate Cancer
3. Early Detection of Lung Cancer
4. Early Detection of Prostate Cancer
5. Early Detection Of Facial Abnormalities in a Fetus
6. Early Detection Of Lung Cancer
7. Early Detection Of Breast Cancer
8. Do Not Neglect Your Abdominal Pain/ Swelling - Help Your Doctor In Early Cancer Detection
9. Patient Negligence - Significant Problem In The Early Detection Of Breast Cancer
10. Digital Mammogram For Detection Of Breast Cancer In Women With Dense Breasts
11. Early Detection Of Prostate Cancer For Complete Recovery From Prostate Cancer
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/29/2017)... ... March 29, 2017 , ... In the United States alone, ... each year develop other types of metastatic brain tumors(3). Though most meningiomas are benign, ... efforts focus on finding more effective treatment options, the San Diego Gamma Knife ...
(Date:3/29/2017)... ... ... Youth Futures International (YFI) premiered its Serve, Learn, & Empower program in 2009 ... have participated in the program every summer. The 2017 Serve, Learn, & Empower ... Visit http://www.ghana.yfiexperience.org to learn more. , “I have sent my daughter ...
(Date:3/29/2017)... ... March 29, 2017 , ... The Wall ... referral to new patients from Burnaby, BC. Patients in need of experienced orthodontics, ... services, can see the esteemed team at Wall Centre Dental. Drs. Parviz Roshan, ...
(Date:3/29/2017)... Minn. (PRWEB) , ... March 29, 2017 , ... ... medical office buildings (MOBs) and other outpatient facilities, and who are the most ... exclusively at those questions, Revista and Healthcare Real Estate Insights (HREI) found that ...
(Date:3/29/2017)... (PRWEB) , ... March 29, 2017 , ... ... in many scientific laboratories. The assembly protocols involve many repetitive steps and often ... for automation, which enables the high-throughput needed, and results in a lower error ...
Breaking Medicine News(10 mins):
(Date:3/30/2017)... -- The new research portal will provide ... of company reports on UnitedHealth Group , as ... activities in the healthcare industry. ...      (Logo: http://photos.prnewswire.com/prnh/20160330/349511LOGO) ... through a fundamental transformation globally-a shift from a volume-based ...
(Date:3/30/2017)... 2017  Purcell Julie & Lefkowitz LLP, a class action ... potential breach of fiduciary duty claim involving the board of ... If you are a shareholder of Synergy ... investigation, free of charge, please visit us at: ... http://pjlfirm.com/synergy-pharmaceuticals-inc/ ...
(Date:3/29/2017)... 2017 Research and Markets has announced the ... to their offering. ... The global gas chromatograph market to grow at a CAGR ... Global Gas Chromatograph Market 2017-2021, has been prepared based on an ... the market landscape and its growth prospects over the coming years. ...
Breaking Medicine Technology: