Navigation Links
DNA-based Detection of Heavy Metals

Researchers at the University of Illinois at Urbana-Champaign have developed a simple, disposable sensor for detecting hazardous uranium ions,// with sensitivity that rivals the performance of much more sophisticated laboratory instruments.

The sensor provides a fast, on-site test for assessing uranium contamination in the environment, and the effectiveness of remediation strategies, said Yi Lu, a chemistry professor at Illinois and senior author of a paper accepted for publication in the Proceedings of the National Academy of Sciences, and posted on its Web site.

While most DNA is double stranded, the catalytic DNA Lu's research group uses has a single strand region that can wrap around like a protein. In that single strand, the researchers fashion a specific binding site -- a kind of pocket that can only accommodate the metal ion of choice.

In this case, the researchers chose to detect uranyl, the most soluble species of uranium ion and the one that poses the greatest threat to human life.

To search for the unique sequence of DNA that could distinguish uranyl from other metal ions, the researchers used a combinatorial approach called in vitro selection. Simple and cost-effective, the selection process can sample a very large pool of DNA (up to 1,000 trillion molecules), amplify the desired sequence by the polymerase chain reaction, and introduce mutations to improve performance.

Lu, with collaborators at Illinois, the Construction Engineering Research Laboratory, Oregon State University and Oak Ridge National Laboratory, assembled the uranium sensor and tested it on soils containing varying amounts of uranium.

The presence of uranyl causes catalytic cleavage of the DNA and release of the fluorophore, resulting in a dramatic increase of fluorescence intensity. With a detection sensitivity of 11 parts per trillion, the disposable sensor rivaled the performance of much more sophisticated laboratory instruments.

"This latest success demonstrates that our methodology can be used to make cost-effective sensors for other hazardous metals, as well, with extremely high sensitivity and selectivity," Lu said. "We can also construct sensor arrays that detect and quantify many metal ions simultaneously."

Source-Bio-Bio Technology

Related medicine news :

1. Detection of Cancer
2. Early Detection Of Prostate Cancer
3. Early Detection of Lung Cancer
4. Early Detection of Prostate Cancer
5. Early Detection Of Facial Abnormalities in a Fetus
6. Early Detection Of Lung Cancer
7. Early Detection Of Breast Cancer
8. Do Not Neglect Your Abdominal Pain/ Swelling - Help Your Doctor In Early Cancer Detection
9. Patient Negligence - Significant Problem In The Early Detection Of Breast Cancer
10. Digital Mammogram For Detection Of Breast Cancer In Women With Dense Breasts
11. Early Detection Of Prostate Cancer For Complete Recovery From Prostate Cancer
Post Your Comments:

(Date:11/27/2015)... ... November 27, 2015 , ... An ... way to dispense prescription medications at home, so he invented the patent-pending ELECTRONIC ... and dispense prescription medications. In doing so, it could help to prevent potential ...
(Date:11/27/2015)... ... November 27, 2015 , ... MPWH, the No.1 Herpes-only dating community in the world, revealed ... 1-1 ). More than 3.7 billion people under the age of 50 – or ... to WHO's first global estimates of HSV-1 infection . , "The data shocks us ...
(Date:11/27/2015)... ... November 27, 2015 , ... ... innovative online platform for mental health and wellness consultation, has collaborated with a ... will bridge the knowledge gap experienced by parents and bring advice from parenting ...
(Date:11/27/2015)... ... November 27, 2015 , ... Indosoft Inc., developer ... incorporation of Asterisk 11 LTS (Long Term Support) into its Q-Suite 5.10 product ... Q-Suite 5.10 up-to-date with a version of Asterisk that will receive not only ...
(Date:11/26/2015)... ... November 26, 2015 , ... The ... bioavailability and the need to integrate dose form selection in early phase drug ... the membership organization supporting and bringing together the UK’s emerging life sciences companies, ...
Breaking Medicine News(10 mins):
(Date:11/26/2015)... , November 26, 2015 ... "Self Administration of High Viscosity Drugs" ... ) has announced the addition of the ... to their offering. --> Research and ... of the "Self Administration of High Viscosity ...
(Date:11/26/2015)... DUBLIN , November 26, 2015 /PRNewswire/ ... the addition of the "Radioimmunoassay Market ... User (Hospital, Pharmaceutical Industry, Academics, Clinical Diagnostic ... - Global Forecast to 2020" report ... ) has announced the addition of the ...
(Date:11/25/2015)... , Nov. 25, 2015 ... the addition of the "Global Brain ... their offering. --> ) ... "Global Brain Monitoring Devices Market 2015-2019" ... Research and Markets ( ) has ...
Breaking Medicine Technology: