Navigation Links
Brain protein expression can be damaged with prolonged alcohol exposure

Researchers at the University at Buffalo probing into the effects of alcohol on the brain, and studying the zebra fish as a model, have zeroed in on many proteins in the central nervous system//, that are impacted negatively with severe alcohol exposure. The scientists were now confident about the proteins’ turn out after prolonged alcohol exposure, after they found fish displaying abnormal behavior. Results of the research appeared in the Aug. 15 online edition of the European Journal of Pharmacology.

Five proteins were found to be over expressed and three were found to be under expressed. These proteins are thought to be involved in critical mechanisms such as programmed cell death, cholesterol balance, amino acid metabolism, oxidative stress and signal transduction.

"Identification of proteins that show selective changes in abundance after alcohol exposure has the potential to unlock new pathways both for understanding the mechanisms of alcoholism and alcohol toxicity, as well as its amelioration," said Richard A. Rabin, Ph.D., professor in the UB Department of Pharmacology and Toxicology and senior author on the study.

Senthilkumar Damodaran, doctoral student in pharmacology, is first author.

The study involved 16 long-fin striped zebrafish, in two trials of eight each, which were placed as a group in a tank with ethyl alcohol for four weeks. Rabin said the researchers chose zebrafish because they are easy to breed and maintain, their DNA sequences are similar to that of humans and they are sensitive to alcohol concentrations.

The concentration of alcohol used is similar to alcohol levels observed in humans, Rabin noted, and is comparable to levels reported in several rodent studies.

"Exposing the fish to alcohol for four weeks has the virtue of being a simple system," he said. "The ethanol concentration and length of exposure can be controlled tightly and applied identically to all

subjects. This method also prevents possible complications due to alcohol withdrawal." He noted, however, that it does not reflect the typical situation in humans.

Once in the alcohol solution, the fish were photographed digitally every 30 seconds, and every third image was stored for analysis. Zebrafish normally swim in a cluster in unison, so the procedure allowed nonintrusive monitoring of an inherent behavior.

Processes known as 2-D electrophoresis and MALFI-TOF mass spectrometry were used to identify proteins in the central nervous system that showed increased or decreased expression.

The photographs showed that after a week of alcohol exposure, the fish became less clustered and occupied a larger area of the tank. However, after two weeks of daily exposure, the cluster reformed and behavior returned to normal, suggesting the fish had become alcohol-tolerant.

The abnormal behavior, characterized by increased distance between fish in the group, returned after six weeks exposure, said Rabin, suggesting a progressive increase in behavior disruption.

"We don't know why this change occurred at this point," he said, "but it might indicate accumulated neurotoxic and neurodegenerative effects of chronic alcohol exposure."

Additional researchers on the study were Cynthia A. Dlugos, Ph.D., assistant professor of pathology and anatomical sciences, and Troy D. Wood, Ph.D., professor of chemistry. The research was supported by UB's Interdisciplinary Research and Creative Activities Fund.

The University at Buffalo is a premier research-intensive public university, the largest and most comprehensive campus in the State University of New York.



Source-Eurekalert
'"/>




Related medicine news :

1. Use of Cellular Phones associated with Increased risk of Brain Tumors
2. Brain death – How to cope with it
3. “Brain fingerprinting”- The new lie detectr
4. Nasal Spray Could Take Drugs Direct to Brain.
5. Virus Combats Brain Tumour
6. Nasal Spray Could Take Drugs Directly to Brain
7. Control of anger disorder connected to Brain Dysfunction
8. High Levels of Protein Linked to Brain Shrinkage
9. Brain damage affects artistic skill
10. Brain cells protected by new compounds
11. Brain changes observed in people with sleep apnoea
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/25/2016)... ... June 25, 2016 , ... As a lifelong Southern Californian, ... and his M.D from the David Geffen School of Medicine at UCLA. He trained ... Angeles to complete his fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program where ...
(Date:6/24/2016)... Angeles, CA (PRWEB) , ... June 24, 2016 , ... ... surgery procedures that most people are unfamiliar with. The article goes on to state ... procedures, but also many of these less common operations such as calf and cheek ...
(Date:6/24/2016)... ... June 24, 2016 , ... ... Sessions in Dallas that it will receive two significant new grants to support ... as PHA marked its 25th anniversary by recognizing patients, medical professionals and scientists ...
(Date:6/24/2016)... ... ... of Topricin and MyPainAway Pain Relief Products, join The ‘Business for a Fair Minimum Wage’ ... 2020 and then adjusting it yearly to increase at the same rate as the median ... floor does not erode again, and make future increases more predictable. , The company is ...
(Date:6/24/2016)... ... June 24, 2016 , ... Puradigm® ... Group (TGIG), has initiated cultivation and processing operations at its production facility, and ... Nevada. , Puradigm is the manufacturer of a complete system of proactive air ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016 Any dentist who has ... of the current process. Many of them do not even ... technical difficulties and high laboratory costs involved. And those who ... it at such a high cost that the majority of ... Dr. Parsa Zadeh , founder of Dental Evolutions ...
(Date:6/23/2016)... June 23, 2016  In a startling report released today, ... residents by lacking a comprehensive, proven plan to eliminate prescription opioid ... ranking of how states are tackling the worst drug crisis in ... states – Kentucky , New Mexico ... . Of the 28 failing states, three – ...
(Date:6/23/2016)... , June 23, 2016 Research ... MEMS Devices Medical Market Analysis 2016 - Forecast to 2022" ... The report contains up to date financial data ... analysis. Assessment of major trends with potential impact on the ... analysis of market segmentation which comprises of sub markets, regional ...
Breaking Medicine Technology: