Navigation Links
Brain protein expression can be damaged with prolonged alcohol exposure

Researchers at the University at Buffalo probing into the effects of alcohol on the brain, and studying the zebra fish as a model, have zeroed in on many proteins in the central nervous system//, that are impacted negatively with severe alcohol exposure. The scientists were now confident about the proteins’ turn out after prolonged alcohol exposure, after they found fish displaying abnormal behavior. Results of the research appeared in the Aug. 15 online edition of the European Journal of Pharmacology.

Five proteins were found to be over expressed and three were found to be under expressed. These proteins are thought to be involved in critical mechanisms such as programmed cell death, cholesterol balance, amino acid metabolism, oxidative stress and signal transduction.

"Identification of proteins that show selective changes in abundance after alcohol exposure has the potential to unlock new pathways both for understanding the mechanisms of alcoholism and alcohol toxicity, as well as its amelioration," said Richard A. Rabin, Ph.D., professor in the UB Department of Pharmacology and Toxicology and senior author on the study.

Senthilkumar Damodaran, doctoral student in pharmacology, is first author.

The study involved 16 long-fin striped zebrafish, in two trials of eight each, which were placed as a group in a tank with ethyl alcohol for four weeks. Rabin said the researchers chose zebrafish because they are easy to breed and maintain, their DNA sequences are similar to that of humans and they are sensitive to alcohol concentrations.

The concentration of alcohol used is similar to alcohol levels observed in humans, Rabin noted, and is comparable to levels reported in several rodent studies.

"Exposing the fish to alcohol for four weeks has the virtue of being a simple system," he said. "The ethanol concentration and length of exposure can be controlled tightly and applied identically to all

subjects. This method also prevents possible complications due to alcohol withdrawal." He noted, however, that it does not reflect the typical situation in humans.

Once in the alcohol solution, the fish were photographed digitally every 30 seconds, and every third image was stored for analysis. Zebrafish normally swim in a cluster in unison, so the procedure allowed nonintrusive monitoring of an inherent behavior.

Processes known as 2-D electrophoresis and MALFI-TOF mass spectrometry were used to identify proteins in the central nervous system that showed increased or decreased expression.

The photographs showed that after a week of alcohol exposure, the fish became less clustered and occupied a larger area of the tank. However, after two weeks of daily exposure, the cluster reformed and behavior returned to normal, suggesting the fish had become alcohol-tolerant.

The abnormal behavior, characterized by increased distance between fish in the group, returned after six weeks exposure, said Rabin, suggesting a progressive increase in behavior disruption.

"We don't know why this change occurred at this point," he said, "but it might indicate accumulated neurotoxic and neurodegenerative effects of chronic alcohol exposure."

Additional researchers on the study were Cynthia A. Dlugos, Ph.D., assistant professor of pathology and anatomical sciences, and Troy D. Wood, Ph.D., professor of chemistry. The research was supported by UB's Interdisciplinary Research and Creative Activities Fund.

The University at Buffalo is a premier research-intensive public university, the largest and most comprehensive campus in the State University of New York.



Source-Eurekalert
'"/>




Related medicine news :

1. Use of Cellular Phones associated with Increased risk of Brain Tumors
2. Brain death – How to cope with it
3. “Brain fingerprinting”- The new lie detectr
4. Nasal Spray Could Take Drugs Direct to Brain.
5. Virus Combats Brain Tumour
6. Nasal Spray Could Take Drugs Directly to Brain
7. Control of anger disorder connected to Brain Dysfunction
8. High Levels of Protein Linked to Brain Shrinkage
9. Brain damage affects artistic skill
10. Brain cells protected by new compounds
11. Brain changes observed in people with sleep apnoea
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/26/2017)... York, NY (PRWEB) , ... May 26, 2017 , ... ... Health & Wound Care" campaign in USA Today, which will educate readers on how ... side of the campaign, a large focus is placed on melanoma. Dancing with the ...
(Date:5/26/2017)... , ... May 26, 2017 , ... Amir Qureshi, MD ... a powerfully versatile, patient-centric spinal cord stimulation system. The Nuvectra™ Algovita SCS System has ... are pleased to be the first in Arkansas to introduce the most powerful SCS ...
(Date:5/26/2017)... (PRWEB) , ... May 26, 2017 , ... ... the first ever copper, antimicrobial, mesh back 24/7 task chair specifically designed for ... applications. “We are thrilled to partner with Cupron® to provide customers with ...
(Date:5/26/2017)... Illinois (PRWEB) , ... May 26, 2017 , ... Silver ... The community, which is located on more than four acres of land at 5620 ... independence. , The 103,000 square-foot building includes 125 studio and one-bedroom apartments. Each ...
(Date:5/26/2017)... ... ... “THE FLINTHILLS FAMILY-Our Journey to the Cross”: the personal journey of Bob Massey and his ... of published authors, Bob and Margaret Massey. Bob Massey is small in stature but ... leather tough." His love for others is apparent in all of his life decisions. ...
Breaking Medicine News(10 mins):
(Date:5/6/2017)... N.Y. , May 5, 2017  Hill-Rom Holdings, ... will add approximately 100,000 square feet to its Welch ... September 2016 its commitment to bring more than 100 ... , where Welch Allyn has maintained a significant presence ... help accommodate these new positions, a large portion of ...
(Date:5/4/2017)... CAESAREA, Israel , May 4, 2017  DarioHealth ... with mobile health and big data solutions, today announced ... an A&D Company, and STI Technologies Limited to lower ... effective immediately. Through STI,s innoviCares card, which is available ... , DarioHealth customers will be eligible for additional ...
(Date:5/3/2017)... 3, 2017  Getinge, a leading global provider ... enhancement and cost efficiency within healthcare and life ... contemporary practice demonstrating that intra-aortic balloon counterpulsation (IABC) ... ill patients. The single-center, retrospective, observational study showed ... MEGA ® 50cc intra-aortic balloon (IAB) in ...
Breaking Medicine Technology: