Navigation Links
Brain protein expression can be damaged with prolonged alcohol exposure

Researchers at the University at Buffalo probing into the effects of alcohol on the brain, and studying the zebra fish as a model, have zeroed in on many proteins in the central nervous system//, that are impacted negatively with severe alcohol exposure. The scientists were now confident about the proteins’ turn out after prolonged alcohol exposure, after they found fish displaying abnormal behavior. Results of the research appeared in the Aug. 15 online edition of the European Journal of Pharmacology.

Five proteins were found to be over expressed and three were found to be under expressed. These proteins are thought to be involved in critical mechanisms such as programmed cell death, cholesterol balance, amino acid metabolism, oxidative stress and signal transduction.

"Identification of proteins that show selective changes in abundance after alcohol exposure has the potential to unlock new pathways both for understanding the mechanisms of alcoholism and alcohol toxicity, as well as its amelioration," said Richard A. Rabin, Ph.D., professor in the UB Department of Pharmacology and Toxicology and senior author on the study.

Senthilkumar Damodaran, doctoral student in pharmacology, is first author.

The study involved 16 long-fin striped zebrafish, in two trials of eight each, which were placed as a group in a tank with ethyl alcohol for four weeks. Rabin said the researchers chose zebrafish because they are easy to breed and maintain, their DNA sequences are similar to that of humans and they are sensitive to alcohol concentrations.

The concentration of alcohol used is similar to alcohol levels observed in humans, Rabin noted, and is comparable to levels reported in several rodent studies.

"Exposing the fish to alcohol for four weeks has the virtue of being a simple system," he said. "The ethanol concentration and length of exposure can be controlled tightly and applied identically to all

subjects. This method also prevents possible complications due to alcohol withdrawal." He noted, however, that it does not reflect the typical situation in humans.

Once in the alcohol solution, the fish were photographed digitally every 30 seconds, and every third image was stored for analysis. Zebrafish normally swim in a cluster in unison, so the procedure allowed nonintrusive monitoring of an inherent behavior.

Processes known as 2-D electrophoresis and MALFI-TOF mass spectrometry were used to identify proteins in the central nervous system that showed increased or decreased expression.

The photographs showed that after a week of alcohol exposure, the fish became less clustered and occupied a larger area of the tank. However, after two weeks of daily exposure, the cluster reformed and behavior returned to normal, suggesting the fish had become alcohol-tolerant.

The abnormal behavior, characterized by increased distance between fish in the group, returned after six weeks exposure, said Rabin, suggesting a progressive increase in behavior disruption.

"We don't know why this change occurred at this point," he said, "but it might indicate accumulated neurotoxic and neurodegenerative effects of chronic alcohol exposure."

Additional researchers on the study were Cynthia A. Dlugos, Ph.D., assistant professor of pathology and anatomical sciences, and Troy D. Wood, Ph.D., professor of chemistry. The research was supported by UB's Interdisciplinary Research and Creative Activities Fund.

The University at Buffalo is a premier research-intensive public university, the largest and most comprehensive campus in the State University of New York.



Source-Eurekalert
'"/>




Related medicine news :

1. Use of Cellular Phones associated with Increased risk of Brain Tumors
2. Brain death – How to cope with it
3. “Brain fingerprinting”- The new lie detectr
4. Nasal Spray Could Take Drugs Direct to Brain.
5. Virus Combats Brain Tumour
6. Nasal Spray Could Take Drugs Directly to Brain
7. Control of anger disorder connected to Brain Dysfunction
8. High Levels of Protein Linked to Brain Shrinkage
9. Brain damage affects artistic skill
10. Brain cells protected by new compounds
11. Brain changes observed in people with sleep apnoea
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/13/2017)... Ill. (PRWEB) , ... October ... ... Edwardsville School of Pharmacy (SOP) alumni Hannah Randall, PharmD ‘17, and Jennifer ... healthcare professionals on guideline updates for the primary prevention of cardiovascular diseases ...
(Date:10/13/2017)... ... October 13, 2017 , ... Apple Rehab Shelton ... staged a mock evacuation of the facility as part of a disaster drill on ... Echo Hose EMS and Shelton City Emergency Manager, as well as the Connecticut ...
(Date:10/13/2017)... ... , ... The Visiting Nurse Association (VNA) of Somerset Hills is proud to ... unique items from across the nation, this holiday-themed event will raise funds and awareness ... VNA. The boutique will be open Saturday, November 4 (10:00 a.m. – 5:00 ...
(Date:10/13/2017)... ... October 13, 2017 , ... Global Healthcare Management’s 4th Annual Kids Fun ... NJ. This free event, sponsored by Global Healthcare Management’s CEO, Jon Letko, is ... is geared towards children of all ages; it is a non-competitive, non-timed event, which ...
(Date:10/13/2017)... , ... October 13, 2017 , ... ... be giving viewers the lowdown on sciatica in a new episode of "Success ... focuses on current events and innovation and investigates each subject in-depth with passion ...
Breaking Medicine News(10 mins):
(Date:10/4/2017)... OBP Medical , a leading ... today announced regulatory approval from Brazil,s ... Nacional de Vigilância Sanitária (ANVISA)) to market ... with integrated LED light source and smoke evacuation ... of a tissue pocket or cavity during surgical ...
(Date:10/2/2017)... Denmark , Oct. 2, 2017 The Rebound ... in the struggle to reverse the tide of prescription drug ... for regulating their medicine intake and stepping down their dosage ... set to launch in December 2017; the first 100,000 people ... Learn more at http://www.rebound-solution.com/ ...
(Date:9/25/2017)... --  Montrium , an industry leader in powerful ... Trial Master Files & Inspection Readiness Conference ( ... Services has selected eTMF Connect to ... a leading European contract research organization (CRO), will ... enable greater collaboration with sponsors, improve compliance and ...
Breaking Medicine Technology: