Navigation Links
An Infectious Agent of Deception, Exposed Through Proteomics

Salmonella bacteria, infamous for food poisoning that kills hundreds of thousands worldwide, infect by stealth. They slip unnoticed into and multiply inside macrophages, the very immune system// cells the body relies on to seek and destroy invading microbes.

Just how Salmonella escapes detection by macrophages, turning predator cells to prey complicit in promoting infection, has seemed impossibly complicated, a needle-in-a-haystack proposition involving thousands of proteins, the building blocks that carry out cells’ vital functions.

Applying the high-volume sorting and analytical power of proteomics—a detailed survey of microbial proteins present in the 24 hours that follow mouse-macrophage infection—a team led by Liang Shi of the Department of Energy’s Pacific Northwest National Laboratory in Richland, Wash., has turned up a suspect protein.

The discovery of the protein, dubbed STM3117, is detailed today (Sept. 29) in The Journal of Biological Chemistry. Knocking out the gene that codes for STM3117, the researchers subsequently crippled the microbe’s ability to multiply inside macrophages. Shi and colleagues say the protein and two closely related proteins discovered in the study are similar in genetic sequence to those known to make and modify chemicals in the microbe’s cell wall called peptidoglycan.

Drug and vaccine designers armed with this mouse-model information can target chemicals or immune responses that disrupt peptidoglycan synthesis and other processes linked to Salmonella’s colonization of macrophages in humans, said Joshua Adkins, a co-author on Shi’s paper and lead author of a related study in Molecular & Cellular Proteomics last month. A quick identification of these proteins, Adkins added, could help physicians assess the virulence of a given strain.

The candidate proteins were winnowed from among 315 possibilities that emerged through a combination of techniques, culminating in measureme nts by Fourier-transform mass spectrometry, or FT-MS. A suite of FT-MS instruments customized by co-author and PNNL-based Battelle Fellow Richard D. Smith enabled the team to rapidly separate and identify many proteins at once even as macrophages were being infected.

Most of the initial candidates were designated “house-keeping” proteins, or those whose numbers relative to other proteins remained more or less constant during the course of infection. But 39 proteins shot up in number during bacterial colonization of macrophages, and of those, a handful or so—including STM3117—responded specifically to a macrophage protein associated with resistance to microbial infection. A standard assay called Western blot confirmed the abundance increases of that small group of proteins during infection.

Source-Newswis
SRM
'"/>




Related medicine news :

1. Transmission Of Infectious Disease On Planes Analyzed
2. Sex Played A Vital Role For Evolution Of Infectious Diseases
3. Systemic Corticosteroid Therapy can be used for treating all types of Infectious Mononucleosis
4. Infectious Diseases of the Wild to grow
5. Infectious Diseases Reduced in China
6. HIV Becomes More Infectious Due To Human Immune-system Protein
7. G8 Leaders Vows to Combat Infectious Diseases Worldwide
8. Experts Are Optimistic Of Making Waterborne Infectious Diseases A Thing Of Past
9. Europe Faces Threat Of Rise In Infectious Diseases
10. The GreeneChip- First Tool to Provide Differential Diagnosis of Infectious Diseases
11. India’s Need for Specialization in Infectious Diseases Urge
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/21/2017)... , ... April 21, 2017 , ... Providing broad access ... in opposition, but there is a better way to address this problem. , That ... of Pharmacy, Public Policy and Economics at the University of Southern California, who served ...
(Date:4/21/2017)... ... April 21, 2017 , ... Chicago plastic surgeon, ... of osteoma. An osteoma is a benign bony lump located on the forehead usually ... can cause difficulties with sight and pain. Dr. Shah has discovered an approach that ...
(Date:4/21/2017)... , ... April 21, 2017 , ... ... Allegheny County that offers services to communities throughout the greater Pittsburgh metropolitan region, ... charity drive to provide adaptive bicycles for differently-abled children. , Variety the Children’s ...
(Date:4/21/2017)... ... , ... The Patient Advocacy Community of The Beryl Institute presented Eve DeVaro, ... Patient Advocacy Award in recognition of her extraordinary contributions to the field of ... The Beryl Institute’s annual Patient Experience Conference on March 20 in Denver, ...
(Date:4/21/2017)... ... April 21, 2017 , ... ... partnered with O2X , an active lifestyle company that provides Human Performance ... health of firefighters, police offers, first responders, military officers and others in service ...
Breaking Medicine News(10 mins):
(Date:4/20/2017)... , April 20, 2017 Research ... America Pharmaceutical Contract Manufacturing Services Market Analysis By Service (Manufacturing, ... Segment Forecasts, 2014 - 2025" report to their offering. ... The Latin American ... 21.0 billion by 2025 Low drug registration cost ...
(Date:4/19/2017)... Cardiology devices segment is anticipated to reach the highest market ... segment is likely to create absolute $ opportunity of a ... By the end of 2027, Cardiology Devices segment is projected ... expanding at a CAGR of 18.4% over the forecast period. ... reprocessed medical devices market in terms of revenue ...
(Date:4/19/2017)... April 19, 2017  IRIDEX Corporation (Nasdaq: ... results for the first quarter 2017 after the close ... management team will host a corresponding conference call beginning ... Investors interested in listening to the conference call ... callers or (703) 326-3030 for international callers, using conference ...
Breaking Medicine Technology: