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A New Inexpensive Technology for Multiplexed Detection

barcode can be read and the target quantified using a single fluorescent color, which greatly simplifies detection.

The particles are also unique in that they are made of a spongy polymer "hydrogel" called poly(ethylene glycol). That polymer enhances the sensitivity of the test because it is porous, allowing the target molecules to diffuse into it.

For the Science paper, the researchers created particles with DNA probes attached at one end. They demonstrated that the particles could accurately and reproducibly detect the presence of multiple target DNA sequences, and they anticipate similar results with RNA, proteins and cytokines.

The researchers are focusing on bedside diagnostics and "theranostics"-the emerging concept of providing personalized diagnostic therapy. This method for tailoring therapies to each patient could be a breakthrough for treating diseases like cancer and cardiovascular disease. The particles could also be used to genetically profile individual patients and screen for bioterrorism or other hazardous environmental agents.

Source-Eurekalert
PRI
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