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ZyGEM Releases Data Confirming Its prepGEM(R) Bacteria Kit Is a Simple, Universal Method for Extracting DNA from Different Bacteria Species
Date:7/9/2009

ional approaches, yet the lack of a universal DNA extraction method has been a barrier to wider adoption of molecular-based technologies. The speed and ease-of-use afforded by our prepGEM(R) Bacteria kit allows for rapid DNA-based detection and monitoring of different bacteria strains efficiently and reproducibly, without significant investment of time or hardware. These important advantages make the prepGEM(R) Bacteria kit valuable for diverse applications, including infectious disease diagnosis and pathogen monitoring in a variety of settings, such as vaccine development and quality control."

In the application note, ZyGEM researchers describe their experience using prepGEM(R) Bacteria to extract DNA from a single sample of mixed bacteria containing Gram positive, Gram negative and polysaccharide-secreting bacterial species that would typically require separate extraction procedures. The prepGEM(R) Bacteria kit extracted PCR-ready DNA from this diverse sample with a single temperature-controlled reaction using its unique combination of thermophilic enzymes and a buffer with proprietary agents that enhance cell lysis. Results showed that prepGEM(R) Bacteria's simple, two-step reaction in a single closed tube efficiently extracted high quality DNA from diverse bacteria sample types, greatly reducing the risk of contamination and providing a method that is easily adaptable for automation.

ZyGEM researchers also successfully extracted quality PCR-ready DNA from low-volume samples using prepGEM(R) Bacteria kits, without the need for the concentration step that is often required before extracting DNA from small samples. This simplifies and speeds up the procedure while also reducing the possibility of sample contamination during the concentration process.

The prepGEM(R) Bacteria kit is compatible with all PCR detection and amplification reagents, and the stability
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SOURCE ZyGEM Corp. Ltd.
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