Navigation Links
iNOS expression may links chronic biliary inflammation to malignant transformation

It is well known that chronic biliary inflammation is a risk factor for biliary carcinogenesis. However, the molecular mechanisms of biliary carcinogenesis as a consequence of chronic biliary inflammation remain unclear.

Inducible nitric oxide synthase (iNOS) is induced in inflamed tissues and generates large amounts of nitric oxide (NO) that can promote mutagenic changes in DNA through DNA oxidization and protein nitrosylation.

In a research article to be published on December 21 in the World Journal of Gastroenterology, Dr. Kitasato and colleagues investigated the role of iNOS activation, NO generation, and DNA damage as the link between chronic inflammation and biliary carcinogenesis, utilizing normal hamster gallbladder epithelial cells cultured in the presence of inflammatory cytokines.

NO generation was increased significantly following cytokine stimulation. Furthermore, the production of NO in the presence of inflammatory cytokines was completely suppressed by the addition of an iNOS inhibitor. The expression of iNOS mRNA in the gallbladder epithelial cells was clearly demonstrated in the presence of cytokine-stimulation, compared with that in the absence of stimulatory cytokines. Moreover, NO-dependent DNA damage was increased significantly by cytokines, and decreased to control levels by an iNOS inhibitor.

In this study, the researchers used primary epithelial cells isolated from hamster gallbladders. These normal epithelial cells are suitable for estimating the involvement of iNOS and NO in biliary carcinogenesis, especially in the initiation of biliary carcinoma as a response to chronic inflammation.

In normal hamster gallbladder epithelial cells, cytokine stimulation induced iNOS expression and NO generation, which was sufficient to cause DNA damage. The results of this study suggest that NO-mediated genotoxicity induced by inflammatory cytokines through activation of iNOS is involved in the process of biliary carcinogenesis in response to chronic inflammation of the biliary tree. These findings represent a new link between infection and carcinogenesis that may be applied broadly beyond the biliary tract.


Contact: Jing Zhu
World Journal of Gastroenterology

Related medicine news :

1. Altered expression of ultraconserved noncoding RNAs linked to human leukemias and carcinomas
2. 10th Anniversary of Revolutionary Teaching Approach that Offers the Severely Disabled the Gift of Self-Expression - Through Painting
3. New study shows that therapeutic gene expression can be sustainable for 1 year
4. Xceed Molecular Launches Ziplex(R) Automated Gene-Expression System at the Association for Molecular Pathology, Booth 84/85
5. Flip-flopping gene expression can be advantageous
6. Facial expressions have greater impact on kids with bipolar disorder
7. Biolytix to Offer Industrial Gene Expression Analysis and Genotyping Services Using Affymetrix GeneChip(R) Technology
8. Pathway links inflammation, angiogenesis and breast cancer
9. Research Links TV/Video Game Playing With Child Obesity; Health Experts Back a New Approach
10. Study links education to risk of cancer death
11. Study Links Coronary Disease, Colon Cancer
Post Your Comments:
(Date:6/27/2016)... ... ... TherapySites, the leading website and online marketing ... Association. This new relationship allows TherapySites to continue to extend their online ... and promotional offers. , "TCA is extremely excited about this new partnership, as ...
(Date:6/26/2016)... ... June 26, 2016 , ... On June 10-11, 2016, A Forever ... Cereal Festival and World’s Longest Breakfast Table in Battle Creek, MI, where the rehabilitation ... as home to some of the world’s leading providers of cereal and other breakfast ...
(Date:6/25/2016)... D.C. (PRWEB) , ... June 25, 2016 , ... ... discuss health policy issues and applications at AcademyHealth’s Annual Research Meeting June 26-28, ... their work on several important health care topics including advance care planning, healthcare ...
(Date:6/25/2016)... ... June 25, 2016 , ... As a lifelong Southern Californian, ... and his M.D from the David Geffen School of Medicine at UCLA. He trained ... Angeles to complete his fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program where ...
(Date:6/24/2016)... ... June 24, 2016 , ... Those who have experienced traumatic events ... turn to unhealthy avenues, such as drug or alcohol abuse, as a coping mechanism. ... tools for healthy coping following a traumatic event. , Trauma sufferers tend to feel ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016 Any dentist who has ... of the current process. Many of them do not even ... technical difficulties and high laboratory costs involved. And those who ... it at such a high cost that the majority of ... Dr. Parsa Zadeh , founder of Dental Evolutions ...
(Date:6/23/2016)... Roche (SIX: RO, ROG; OTCQX: RHHBY) announced that it ... (procalcitonin) assay as a dedicated testing solution for people ... Roche is the first IVD company in the U.S ... assessment and management. PCT is a sepsis-specific ... blood can aid clinicians in assessing the risk of ...
(Date:6/23/2016)... DUBLIN , June 23, 2016 ... "Key Pharma News Issue 52" report to their offering. ... need in influenza treatment creates a favourable commercial environment for ... and growing patient base that will serve to drive considerable ... flu vaccine would serve to cap sales considerably, but development ...
Breaking Medicine Technology: