Navigation Links
Zebrafish may help speed drug discovery
Date:1/17/2012

AUGUSTA, Ga. Tiny zebrafish just may give scientists one solution to information overload in the search for new drugs therapies.

High throughput screening, which uses robotics and computers to rapidly screen drugs, genes or proteins, to identify, for example, compounds that are best at destroying cancer or restoring insulin-producing cells. The technology has both revolutionized and stymied research, said Dr. Jeffrey Mumm, biologist in the Medical College of Georgia at Georgia Health Sciences University.

While the decade-old technology can screen 100,000 compounds in a day, there is no efficient next step for whittling to a manageable number the resulting say, 2,500 compounds that on first blush appear to have potential. Consequently, drug discovery has actually stalled, creating a scientific "indigestion," Mumm said. Drug failure rates and discovery costs, on the other hand, have escalated.

"The next best thing to do is probably take all the compounds and put them into an animal model that mimics the disease you are trying to cure but nobody has that kind of budget or would know where to start with that number of candidates," Mumm said.

One solution is pairing zebrafish with reporter-based assays that make certain parts glow, according to Mumm's research published in the journal PLoS ONE. He calls it Automated Reporter Quantification.

"It's a way to cut to the chase rather than having to make educated guess about which of the 2,500 compounds are really of interest," said Mumm. Zebrafish, which start out as single cell organisms that are fully functioning by day four, already are an invaluable research model for a wide variety of diseases, such as Mumm's efforts to regenerate cells in the face of degenerative diseases of the eyes or pancreas.

To produce a model of type 1 diabetes, for example, he uses a single drug to destroy insulin-producing cells; 24 hours later, he knows those cells are gone because the glow is gone in the transparent zebrafish.

Now, he can leave the fish in the well plates, which look like plastic ice trays with cubes about the size of pencil tops and come in multiples of 96. The individual wells enable him to quickly reassess the impact of the thousands of compounds identified by high-throughput screening in a living specimen. Simply put: Does the glow come back? The next step becomes logical and doable: study the 20 or so compounds that continued to show promise in a mouse model. These steps to the fish then mouse, called biological validation, have been a real bottleneck in science post high-throughput screening, Mumm said. "This is going to give you much more thorough information about how compounds affect the overall physiology of a living being," Mumm said.

He noted that reporter-based assays number in the thousands so what they can help find is essentially limited only by the imagination.

He's used one to monitor a major signaling pathway that consistently shows up in cancer. "We hooked the pathway up to a reporter that told us whether it was on or off, then tested whether we could modulate it over time and, sure enough, it was very easy to do," Mumm said. "Name the cancer and basically you have WNT activated so there is a huge effort in cancer therapeutics to try and essentially shut it down."
'/>"/>

Contact: Toni Baker
tbaker@georgiahealth.edu
706-721-4421
Georgia Health Sciences University
Source:Eurekalert  

Related medicine news :

1. EMBL picture release: Developing zebrafish patchwork
2. Zebrafish popular with researchers
3. Researchers use zebrafish to identify new gene linked to melanoma
4. Alzheimers Foundation of America Applauds Social Security for Speeding Disability Benefits for Early-Onset Alzheimers Disease
5. Pro Athletes Brains React at Olympic Speed
6. USA's Greatest Speed Skater Takes His Medical Practice to the Cutting Edge
7. Keeping up with the neighbors speeds vaccine use
8. Bees see super color at super speed
9. After Teeth Are Pulled, Platelet-Rich Plasma May Speed Healing
10. Nausea and speeding heart can be signs of flu in pregnant women
11. Getting Help with Initial Social Security Disability Application Can Speed Access to Benefits
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Zebrafish may help speed drug discovery
(Date:3/30/2017)... ... , ... An inventor and manicurist from Greet, S.C., wants to offer people a new way ... maintain clean and healthy feet, so I know the importance of proper foot care," he ... designed the FOOT-TRAN SYSTEM." , The FOOT-TRAN SYSTEM enables a user to clean and exfoliate ...
(Date:3/30/2017)... ... March 30, 2017 , ... This ... to President Trump expressing their support for an independent Vaccine Safety Commission. Five ... and one medical journalist, will hold a press conference at 9:00 AM ...
(Date:3/29/2017)... (PRWEB) , ... March 29, 2017 , ... In the ... to 300,000 people each year develop other types of metastatic brain tumors(3). Though most ... the brain(3). As efforts focus on finding more effective treatment options, the San ...
(Date:3/29/2017)... ... ... the main cause of hay fever in the United States, with an estimated 95 percent ... to July each year; with the worst time for sufferers being June and July. ... ) provide an effective defense against grass pollen; they are proven in independent studies to ...
(Date:3/29/2017)... St. Petersburg, FL (PRWEB) , ... March 30, 2017 , ... ... Often called the "Spice of Life" or "Wonder Spice", it has been used for ... extensive use in the East," says Heshelow, author of " Turmeric: How to Use ...
Breaking Medicine News(10 mins):
(Date:3/29/2017)... 29, 2017 Research and Markets has announced the ... Treated at Proton Therapy Centers & Forecast" report to their offering. ... The South Korean Proton ... market size in 2016. The Untapped Proton Therapy Market for ... in 2016. Proton Therapy plays an important role in ...
(Date:3/29/2017)... , March 29, 2017  Experts in the ... monitoring devices like  Soberlink Systems  as a model ... paper, published in early 2017, concluded that remote ... in managing patient recovery." The findings ... of Addiction Medicine, detail a range of variables ...
(Date:3/29/2017)... Calif. , March 29, 2017 ... for patients as the highest standard in quality ... Krypton Vision introduces its objective Wavefront-driven refraction technologies ... subjective approach. This enables an unprecedented level of ... - exceptional vision relegated to Topgun Navy fighter ...
Breaking Medicine Technology: