Navigation Links
Yale researchers use genetic code to engineer a living protein
Date:8/25/2011

Yale University researchers have successfully re-engineered the protein-making machinery in bacteria, a technical tour de force that promises to revolutionize the study and treatment of a variety of diseases.

"Essentially, we have expanded the genetic code of E. coli, which allows us synthesize special forms of proteins that can mimic natural or disease states," said Jesse Rinehart of the Department of Cellular and Molecular Physiology and co-corresponding author of the research published in the August 26 issue of the journal Science.

Since the structure of DNA was revealed in the 1950s, scientists have been working hard to understand the nature of the genetic code. Decades of research and recent advances in the field of synthetic biology have given researchers the tools to modify the natural genetic code within organisms and even rewrite the universal recipe for life.

"What we have done is taken synthetic biology and turned it around to give us real biology that has been synthesized," Rinehart explained.

The Yale team under the direction of Dieter Sll, Sterling Professor of Molecular Biophysics and Biochemistry, professor of chemistry and corresponding author of the paper developed a new way to influence the behavior of proteins, which carry out almost all of life's functions. Instead of creating something new in nature, the researchers essentially induced phosphorylation, a fundamental process that occurs in all forms of life and can dramatically change a protein's function. The rules for protein phosphorylation are not directly coded in the DNA but instead occur after the protein is made. The Yale researchers fundamentally rewrote these rules by expanding the E. coli genetic code to include phosphoserine, and for the first time directed protein phosphorylation via DNA.

This new technology now enables the production of human proteins with their naturally occurring phosphorylation sites, a state crucial to understanding disease processes. Previously, scientists lacked the ability to study proteins in their phosphorylated or active state. This has hindered research in diseases such as cancer, which is marked by damagingly high levels of protein activation.

"What we are doing is playing with biological switches turning proteins on or off which will give us a completely new way to study disease states and hopefully guide the discovery of new drugs," Rinehart said.

"We had to give some very ancient proteins a few modern upgrades," Sll said.

Sll and Rinehart now are attempting to create proteins in states known to be linked to cancer, type 2 diabetes, and hypertension. Both men, however, stressed the technique can be done for any type of protein.

"Dr. Sll and his colleagues have provided researchers with a powerful new tool to use in uncovering how cells regulate a broad range of processes, including cell division, differentiation and metabolism," said Michael Bender, who oversees protein synthesis grants at the National Institute of General Medical Sciences of the National Institutes of Health.


'/>"/>

Contact: Bill Hathaway
william.hathaway@yale.edu
203-432-1322
Yale University
Source:Eurekalert

Related medicine news :

1. Researchers developing new test to measure risk for birth defects and neuro-developmental disorders
2. Breast-Feeding Wont Prevent Kids Eczema, Researchers Say
3. Government subsidies to the US film industry promote youth smoking, argue researchers
4. Researchers Say Theyve Found Common Cause of All Types of ALS
5. UCSD researchers alarmed at rise in hookah use among California youth
6. Researchers building new sensor that could stop falls by elderly, before they happen
7. AIDS researchers isolate new potent and broadly effective antibodies against HIV
8. Pitt cancer researchers find key oncoprotein in Merkel cell carcinoma
9. Researchers create new experimental vaccine against chikungunya virus
10. USF researchers get $2.6 million NIH grant to investigate new post-stroke therapy
11. Childrens National collaborates with NIH researchers to identify gene variant in Proteus syndrome
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/9/2016)... Heights, NY (PRWEB) , ... December 09, 2016 , ... ... Alcoholism and Drug Dependence, Inc. (NCADD) is recommending the film Whispering Spirits ... states and the District of Columbia as an education tool in the war against ...
(Date:12/9/2016)... ... 09, 2016 , ... An inventor from Cana, Va., wanted to fulfill the ... amusement park rides. , The patent-pending SAFETY STRAP FOR AMPUTEES improves accessibility. It eliminates ... be set up in a matter of minutes, or even seconds. The SAFETY STRAP ...
(Date:12/9/2016)... , ... December 09, 2016 , ... ... at The Pierre Hotel in New York, NY, on December 3rd, to benefit ... and physicians attended the annual event, which raised over $1 million - the ...
(Date:12/9/2016)... ... ... "I had a terrible time trying to get my grandson to use ... nebulizer had a more child-friendly design, then children would be more likely to look ... to avoid the need to deliver medication via a nebulizer mask. The design will ...
(Date:12/8/2016)... Charleston, WV (PRWEB) , ... December 08, 2016 ... ... that it will become Quality Insights beginning January 1, 2017. The name ... emphasizes its commitment to measuring and improving health care quality. , “We are ...
Breaking Medicine News(10 mins):
(Date:12/9/2016)... DUBLIN , Dec 9, 2016 ... "High-Throughput Screening Market - Forecast to 2021" report to ... ... adoption of open innovative models in the pharmaceutical and biotechnology ... spending and rising government funding. Emerging markets and growing research ...
(Date:12/9/2016)... , Dec 9, 2016 Research and Markets ... 2016-2020" report to their offering. ... The global travel vaccines market to grow ... The report covers the present scenario and the growth prospects of ... size, the report considers the revenue generated from the sales of ...
(Date:12/8/2016)... LONDON , Dec. 8, 2016 Australia ... GlobalData,s new report, "Australia Ophthalmic Lasers Market Outlook to ... Lasers market. The report provides value, in millions of ... within market segements - Excimer Lasers, Femtosecond Lasers and ... and distribution shares data for each of these market ...
Breaking Medicine Technology: