Navigation Links
Work with fungus uncovering keys to DNA methylation
Date:12/15/2008

Researchers in a University of Oregon lab have shed more light on the mechanism that regulates DNA methylation, a fundamental biological process in which a methyl group is attached to DNA, the genetic material in cells of living organisms.

DNA methylation is essential for normal growth and development in plants and animals. It has been implicated in long-term memory, and irregularities in its process are associated with diseases such as cancer.

In the UO's Institute of Molecular Biology, Eric U. Selker and members of his laboratory use a quickly reproducing and easy-to-manipulate fungus, Neurospora crassa, to explore the control of DNA methylation. Neurospora is considered the simplest model organism for such research.

Reporting in the Dec. 15 issue of the journal Genes & Development, Selker and Keyur K. Adhvaryu, a postdoctoral researcher in the Selker lab, document that the enzyme protein phosphatase PP1 is necessary for normal methylation of DNA.

In the nucleus of eukaryotic cells, DNA is wrapped around histone proteins to form chromatin, and one histone, H3, turns out to be critical for DNA methylation. "It was long thought that histones were simply structural proteins, but we are learning that these proteins are also informational," Selker said.

This was demonstrated in the journal Nature in 2001 by Selker and his former postdoctoral research associate Hisashi Tamaru. They found that a protein required for DNA methylation, DIM-5, is an enzyme that adds a methyl group onto lysine 9 of histone H3. "This was the first solid indication that chromatin is important for DNA methylation," Selker said.

The new paper by Adhvaryu and Selker shows that PP1 is important to remove phosphates attached to serine 10 of H3, the site immediately adjacent to the site that DIM-5 needs to methylate, leading to DNA methylation.

In an accompanying article in the same issue of Genes & Development, Wolfgang Fischle, a biochemist at the Max-Planck Institute for Biophysical Chemistry, praises the findings of Selker and Adhvaryu. He writes that there appears to be extensive "crosstalk" involved in the chemical modifications that occur on histones to influence other enzymes that interact with chromatin "Adhvaryu and Selker provide novel insights into an intricate regulatory network involving histone phosphorylation, histone methylation and DNA methylation," he noted.

"DNA methylation seems to be a luxury item in Neurospora, which means we can manipulate it as we wish, making mutants that don't do it and thereby identify important players," Selker said. "We are identifying how DNA methylation is controlled and what it does in this organism. Our assumption is that a lot of what we find in Neurospora will be applicable to other systems."

In this case, Selker said, Keyur demonstrated very nicely, in a couple different ways, that protein phosphatase PP1 is required for normal DNA methylation. "DNA methylation is involved in a silencing of invasive DNA as well as a variety of normal genes, including those on the inactive X chromosome, those subjected to imprinting, and well as tumor suppressor genes," he said, adding that methylation of the latter class of genes can lead to cancer.


'/>"/>

Contact: Jim Barlow
jebarlow@uoregon.edu
541-346-3481
University of Oregon
Source:Eurekalert  

Related medicine news :

1. Photo: Scientists Complete Genome Sequence of Fungus Responsible for Dandruff, Skin Disorders
2. Active compounds found in Ganoderma lucidum fungus with potential to treat prostate cancer
3. Fungus Sheds Light on Development of Human Genders
4. Fighting a Fungus That Thrives on Tomatoes
5. Innate immune system targets asthma-linked fungus for destruction
6. New topical approach to treating nail fungus circumvents problems with current therapy
7. UNCOVERING THE TRUTH - A Town Hall Meeting on Mental Health Parity
8. Uncovering the Achilles heel of the HIV-1 envelope
9. Under Wraps: Uncovering the Secrets and Lies of the Holiday Season
10. Mouse model highlights histone methylation as distinguishing feature for leukemia subtypes
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Work with fungus uncovering keys to DNA methylation
(Date:8/15/2017)... ... August 15, 2017 , ... Western University of Health ... will move forward in bold new directions while using its foundational values of ... with Convocation, the traditional opening of the academic year, on August 12, 2017 ...
(Date:8/15/2017)... CA (PRWEB) , ... August 15, 2017 , ... The city of Los Angeles is ... in harmony with “Cali Style”. LA’s Top Style List, a website that spotlights fashion news ... the public wear their wardrobe all year long. , According to the senior editor of ...
(Date:8/15/2017)... ... August 15, 2017 , ... Horizon Goodwill ... McCain said, “We are overwhelmed with gratitude for the very generous donation from ... need assistance to both get a job and keep it. This donation will ...
(Date:8/15/2017)... ... ... Progressive Dental, a leading dental marketing and consulting firm founded in ... Growing Companies in Tampa Bay. The Tampa Bay Business Journal creates this list considering ... growth. For this year’s award, Progressive Dental ranked number 29, with a percentage growth ...
(Date:8/15/2017)... , ... August 15, 2017 , ... ... products, announced today the introduction of the innovative new 10x10 bladder pad ( ... patients. The plush design enhances comfort and enables the patient to enjoy the ...
Breaking Medicine News(10 mins):
(Date:8/10/2017)... 10, 2017  Physical Rehabilitation Network (PRN), acquired the long-standing ... Lakewood, Colorado . The reputable clinic will continue to ... DPT with his staff of four clinicians. Lipkin received his ... brings over 10 years of experience with a strong background ... PT marks the 10th PRN clinic in and around the ...
(Date:8/7/2017)... Insightin Health, provider of data-driven decision-making ... announced the selection of Michael Wood ... as of February 2017. In this role, Wood will ... our clients. Wood brings with him more than ... analytics within the healthcare industry. Wood formerly served ...
(Date:8/7/2017)... Inc. (NYSE: DPLO), the nation,s largest independent specialty pharmacy, announced ... comparisons, unless otherwise noted, are to the quarter ended June ... Quarter 2017 Highlights include: Revenue of ... Total prescriptions dispensed of 220,000, compared to ... Gross profit per prescription dispensed of ...
Breaking Medicine Technology: