Navigation Links
What is the more suitable for early detection of low abundant lamivudine-resistant mutants?
Date:1/16/2008

Lamivudine is an effective antiviral agent for treatment of patients with chronic hepatitis B and advanced liver diseases. However, long-term lamivudine monotherapy leads to the emergence of lamivudine-resistant hepatitis B virus (HBV) mutants in some patients chronically infected with HBV. Sensitive methods for early detection of lamivudine-resistant mutants will help physicians make clinical decisions in treating patients with HBV infection.

To date, many assays have been used for detection of lamivudine-resistant mutants in patients with Hepatitis B. Differences in sensitivity, specificity, cost, and time required, exist in these methods. Real-time PCR is able to quantitatively detect a small portion of resistant mutants in HBV populations and ligase detection reaction (LDR) is a newly developed method for detection of low abundant mutants in the background of wild-type HBV. However, there are no studies which have compared the clinical performance of the two methods.

A research article to be published on January 7 in the World Journal of Gastroenterology (volume 14, issue 1) addresses this question. It compared LDR and real-time PCR for detection of low abundant YMDD mutations in mixed plasmids and serum samples from 52 lamivudine treated patients. Time required and reagent cost for both assays were evaluated. The research was conducted carefully by an experienced team of investigators.

The article suggested both methods are sensitive and inexpensive for detection of YMDD mutation; but LDR is more sensitive than real-time PCR. The results obtained with both methods were completely concordant in all serum samples. LDR was able to detect as low as 0.01% (100 copies/mL) of YIDD plasmid, while real-time PCR only detected 0.1% (1000 copies/mL) of YIDD plasmid in the background of YMDD plasmid. In addition, the cost of LDR is slightly lower than that of real-time PCR.

However, real-time PCR is much more rapid and requires le
'/>"/>

Contact: Jing Zhu
wjg@wjgnet.com
0086-105-908-0039
World Journal of Gastroenterology
Source:Eurekalert

Page: 1 2

Related medicine news :

1. Research suggests new treatment suitable for all patients
2. Pot bellies linked to early signs of cardiovascular disease
3. Eye-staining technique offers early detection for dry eye syndrome
4. Early Weight Loss in Women Linked to Dementia
5. Isolation of a new gene family essential for early development
6. U of M study: Early treatment can reverse heart damage
7. Gerresheimer to be Included in the SDAX Early Decision on Inclusion in the MDAX on September 5, 2007
8. Rock N Roll: Sex, Drugs and an Early Exit
9. Pop stars more than twice as likely to die an early death
10. Exercise and yoga improves quality of life in women with early-stage breast cancer
11. Research says doctors gender may hinder early diagnosis of heart disease in women
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... pm EDT): In a Viewpoint published today in the ... team of Boston researchers call for the implementation of ... United States. The researchers from the Friedman School of ... and Boston Children,s Hospital write that policies taxing nearly ... help people make meaningful dietary changes and substantially reduce ...
(Date:9/2/2014)... TCG, a leading government technology strategy and IT consultancy, ... fastest-growing companies in America for the seventh year in a ... in 2013, 2012, 2011, 2010, 2009, 2008, and the Inc. ... 2010 to 2013, ranking the company at position 3,708 on ... up 635 spots from 2013! More information is on ...
(Date:9/2/2014)... The use of Xarelto (known generically ... at a rapid pace, even as Xarelto lawsuits ... the U.S., Bernstein Liebhard LLP reports. According to a ... a year of its 2011 launch, patients were just ... blood thinner that has been in use for several ...
(Date:9/2/2014)... News) -- Complications are rare among breast cancer ... study indicates. However, the researchers did find ... slightly higher risk for certain complications than a ... 18,000 breast cancer patients who had a single ... reconstruction and were followed for 30 days after ...
(Date:9/2/2014)... September 2, 2014 In a large population-based ... found that mild cognitive impairment (MCI) occurred twice ... type 2. Interestingly, this strong association was only ... older participants (66-80 years) the association vanished. This ... Alzheimer,s Disease . , The concept of MCI ...
Breaking Medicine News(10 mins):Health News:Taxes and subsidies could encourage healthier diet and lower healthcare costs 2Health News:TCG One of the Fastest-Growing Companies in America – for the Eighth Time! 2Health News:Xarelto Lawsuit News: Bernstein Liebhard LLP Comments on Growing Use of Xarelto in Spite of Mounting Product Liability Litigation 2Health News:Xarelto Lawsuit News: Bernstein Liebhard LLP Comments on Growing Use of Xarelto in Spite of Mounting Product Liability Litigation 3Health News:Xarelto Lawsuit News: Bernstein Liebhard LLP Comments on Growing Use of Xarelto in Spite of Mounting Product Liability Litigation 4Health News:Complication Rates Low With Mastectomy, Breast Reconstruction: Study 2Health News:Diabetes mellitus and mild cognitive impairment: Higher risk in middle age? 2
... very practical agents in therapeutic applications. Researchers from ... Institute of Technology, writing Cancer Letters // (Available ... nanospheres as photothermal agent by selectively delivering gold ... a clinically related cancer biomarker, and discuss the ...
... true for the scientists who been working hard for the ... take an extra step ahead as it is likely to ... as their offspring, are safe to eat., ,Canada ... Canadian Scientists having proved themselves following the success regarding Starbuck, ...
... Publication, released on the 5th of October, which comprises ... has a comprehensive report on the threat of chronic ... Union Health Minister Dr Anbumani Ramadoss, the need to ... of chronic diseases in India was highlighted, stating that ...
... Sanofi Pasteur and Merck and found to be effective ... doses over 6 months which has shown 100% successful. The company ... protects against Human Papilloma virus infection and also genital warts, which ... market early next year and the profits would be great, and ...
... to regularly floss your teeth or so your dentist tells ... larger percentage of the population does not indulge in flossing. ... leads to gum problems including bleeding gums, receding gums and ... American Dental Association's (ADA) annual conference in Philadelphia says that ...
... for breast cancer patients, who show positive for Her-2/neu. ... positive, and they have a more resistant and active ... are Her-2/neu positive and the treatment for these patients ... not show any significant disease reduction. These patients should ...
Cached Medicine News:Health News:Gold Nanoparticles: Agents For Noninvasive Cancer Therapy 2Health News:Strategies to prevent the rising tide of the epidemic of chronic diseases 2Health News:Gardasil vaccine for cervical cancer 2
(Date:9/2/2014)... 2, 2014 According to the ... Pre-packed, Analytical, Preparative), by Material (Metal, Glass, Plastic), by ... Forecast to 2018", published by MarketsandMarkets, the Chiral Chromatography ... is poised to grow at a CAGR of 5.2% ... Browse 90 market data tables and 15 ...
(Date:9/2/2014)... , Registry provides new insights ... and treatment satisfaction [ 1 ] ...        (Photo: http://photos.prnewswire.com/prnh/20140902/703596 ... Barcelona, Spain : Daiichi Sankyo today ... events-European Registry in Atrial Fibrillation (PREFER in AF), revealing ...
(Date:9/2/2014)... the Netherlands , Sept. 2, 2014 /PRNewswire/ ... North American operations through its subsidiary HealthLink International, by ... order to cash services for medical device manufacturers, throughout ... ERP platform. Building on the experience we ... Europe with customer service, VAT deferment, ...
Breaking Medicine Technology:Chiral Chromatography Column Market Worth $87.8 Million by 2018 2Chiral Chromatography Column Market Worth $87.8 Million by 2018 3Chiral Chromatography Column Market Worth $87.8 Million by 2018 4One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 2One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 3One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 4One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 5One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 6One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 7One Year Data from the PREFER in AF Registry Show Significant Changes in Atrial Fibrillation (AF) Management in Europe and Implementation of Clinical Guidelines 8HealthLink Europe opens North American medical warehouse operation 2
... is a national reference laboratory and a ... development. ARUP offers an extensive test menu ... in clinical and anatomic pathology. Owned by ... include more than half of the nation's ...
... a national reference laboratory and a worldwide ... ARUP offers an extensive test menu of ... clinical and anatomic pathology. Owned by the ... more than half of the nation's university ...
... reference laboratory and a worldwide leader in ... an extensive test menu of highly complex ... anatomic pathology. Owned by the University of ... half of the nation's university teaching hospitals ...
... is a national reference laboratory and a ... development. ARUP offers an extensive test menu ... in clinical and anatomic pathology. Owned by ... include more than half of the nation's ...
Medicine Products: