Navigation Links
What is the more suitable for early detection of low abundant lamivudine-resistant mutants?
Date:1/16/2008

Lamivudine is an effective antiviral agent for treatment of patients with chronic hepatitis B and advanced liver diseases. However, long-term lamivudine monotherapy leads to the emergence of lamivudine-resistant hepatitis B virus (HBV) mutants in some patients chronically infected with HBV. Sensitive methods for early detection of lamivudine-resistant mutants will help physicians make clinical decisions in treating patients with HBV infection.

To date, many assays have been used for detection of lamivudine-resistant mutants in patients with Hepatitis B. Differences in sensitivity, specificity, cost, and time required, exist in these methods. Real-time PCR is able to quantitatively detect a small portion of resistant mutants in HBV populations and ligase detection reaction (LDR) is a newly developed method for detection of low abundant mutants in the background of wild-type HBV. However, there are no studies which have compared the clinical performance of the two methods.

A research article to be published on January 7 in the World Journal of Gastroenterology (volume 14, issue 1) addresses this question. It compared LDR and real-time PCR for detection of low abundant YMDD mutations in mixed plasmids and serum samples from 52 lamivudine treated patients. Time required and reagent cost for both assays were evaluated. The research was conducted carefully by an experienced team of investigators.

The article suggested both methods are sensitive and inexpensive for detection of YMDD mutation; but LDR is more sensitive than real-time PCR. The results obtained with both methods were completely concordant in all serum samples. LDR was able to detect as low as 0.01% (100 copies/mL) of YIDD plasmid, while real-time PCR only detected 0.1% (1000 copies/mL) of YIDD plasmid in the background of YMDD plasmid. In addition, the cost of LDR is slightly lower than that of real-time PCR.

However, real-time PCR is much more rapid and requires less manual work than LDR. The total assay time for LDR and real-time PCR was 4.5 and 2.5 h, respectively. Another advantage of the real-time PCR method is it is able to calculate the ratio of mutants to total virus in samples. This will be useful in clinical studies on the dynamics of resistant mutants during lamivudine therapy.


'/>"/>

Contact: Jing Zhu
wjg@wjgnet.com
0086-105-908-0039
World Journal of Gastroenterology
Source:Eurekalert

Related medicine news :

1. Research suggests new treatment suitable for all patients
2. Pot bellies linked to early signs of cardiovascular disease
3. Eye-staining technique offers early detection for dry eye syndrome
4. Early Weight Loss in Women Linked to Dementia
5. Isolation of a new gene family essential for early development
6. U of M study: Early treatment can reverse heart damage
7. Gerresheimer to be Included in the SDAX Early Decision on Inclusion in the MDAX on September 5, 2007
8. Rock N Roll: Sex, Drugs and an Early Exit
9. Pop stars more than twice as likely to die an early death
10. Exercise and yoga improves quality of life in women with early-stage breast cancer
11. Research says doctors gender may hinder early diagnosis of heart disease in women
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/29/2016)... ... April 29, 2016 , ... In its efforts of bringing quality senior care ... franchise development and expansion as it welcomes Ferdie Vasquez of the new 1Heart Caregiver ... his 32 years of working in various industries. He had been a Franchise ...
(Date:4/28/2016)... ... 28, 2016 , ... The Dentists Insurance Company ... a letter of intent with Moda to acquire Dentists Benefits Insurance ... Started by dentists 36 years ago, TDIC focuses exclusively on supporting the dental ...
(Date:4/28/2016)... ... April 28, 2016 , ... CastCoverz!, America’s ... with AquaShieldUSA, the country's oldest waterproof cast protector . As the largest ... the largest selection of daily, night, weatherproof and waterproof covers for most orthopedic ...
(Date:4/28/2016)... ... April 28, 2016 , ... ... the organization’s most successful events, attracting medical professionals from around the globe who ... networking events. , Opening Ceremonies Sunday, April 17 included the introduction of the ...
(Date:4/28/2016)... ... April 28, 2016 , ... In many parts of the world, contamination from ... This is particularly true in underdeveloped parts of Africa where clean sources of food ... campus in Tangier, Morocco, will examine this global health issue and consider how it ...
Breaking Medicine News(10 mins):
(Date:4/27/2016)... 2016 Tie-up with Government hospitals ... to save newborns   Fortis La Femme, ... in collaboration with Breast Milk Foundation (BMF), a non-profit organization ... Milk Bank, ,Amaara, in Delhi-NCR today. This non-profit centre recognizes ... infants and should be available to babies deprived of mother,s ...
(Date:4/27/2016)... SAN FRANCISCO , April 27, 2016 /PRNewswire/ ... to reach over USD 2.14 billion by 2022, ... Research, Inc.       (Logo: http://photos.prnewswire.com/prnh/20150105/723757 ... key technological advancement affecting the efficiency and accuracy ... hence, the persistent demand for novel urinalysis instruments ...
(Date:4/26/2016)... US demand for infection prevention products ... annually to $27.6 billion in 2020.  Increasing pressures ... rates of healthcare-associated infections (HAIs) will boost revenues ... Although declining, the overall rate of certain HAIs ... by the CDC.  Recent statistics indicate that only ...
Breaking Medicine Technology: