Navigation Links
What composes the human heart? U of T researchers crunch the numbers
Date:11/20/2013

A foundational study published in top biomedical journal PNAS (Proceedings of the National Academy of Sciences) this week by researchers at the University of Toronto's Institute of Biomaterials & Biomedical Engineering (IBBME) and the McEwen Centre for Regenerative Medicine have identified the optimal structure and cell ratio associated with heart function and the discovery has already led the team to another research first: the engineering of the first-ever living, three-dimensional human arrhythmic tissue.

The study marks the first time that researchers have tried to define and formulate the precise type and ratio of cell types that produce highly functional cardiac tissue.

"Hearts are not just composed of one type of cell," explained fourth-year IBBME PhD Student Nimalan Thavandiran and first author of the PNAS study. But until now, scientists have not known how to mix different cell types in engineered heart tissue in such a way that the tissue achieves the composition and maturity level of the native human heart.

Thavandiran solved this mystery by methodically separating out different cell types derived from human pluripotent stem cells and precisely mixing them back together. Using scoring metrics associated with functional hearts - contraction, electrical activity and cell alignment Thavandiran was able to develop a formula for engineering highly functional heart tissue.

The composition of the cells is vital," stated Thavandiran. "We discovered that a mixture of 25% cardiac fibroblasts (skin-like cells) to 75% cardiomyoctes (heart cells) worked best." The carefully composed cell ratios were then grown in three-dimensional "wires" that mimic the structure of human heart tissue.

"An exciting result of our study is our ability to miniaturize the tissues into human heart micro-tissues that can be used to measure normal and diseased human heart responses to drugs," emphasized Professor Peter Zandstra, corresponding author of the study and Canada Research Chair in Stem Cell Bioengineering at IBBME and the McEwen Centre for Regenerative Medicine.

From discovering the right composition of heart cells, the researchers next designed the first-ever three-dimensional arrhythmia tissue model.

Millions of people experience arrhythmia each year, a condition in which the feedback of electrical pulses of the heart is interrupted, leaving the heart unable to contract and pump blood effectively.

With the right cellular composition, though, the researchers were able to engineer the circular tissue model associated with arrhythmia. The team then applied electrical pulses to the arrhythmic tissues, 'zapping' the irregularly beating tissue into a state of regular contractions.

Since human heart cells aren't easily grown, being able to engineer highly functional heart tissue from human stem cells is a vital concern for cardiac researchers.

"We can now combine this compositional knowledge with electrical stimulation and mechanical stimulation to obtain a truly biomimetic system necessary for cardiac research," cited corresponding author Associate Professor Milica Radisic, core faculty at IBBME and the Department of Chemical Engineering and Applied Science, and Canada Research Chair in Functional Cardiovascular Tissue Engineering.

Stressing the urgent need for highly functional heart tissue to perform important drug screening research, Thavandiran added, "We're making a really big push to bring this model to the marketplace."

The team has been working with the Centre for the Commercialization of Regenerative Medicine (CCRM) to commercialize their tissue modeling platform.


'/>"/>

Contact: Erin Vollick
Comm.ibbme@utoronto.ca
416-946-8019
University of Toronto
Source:Eurekalert

Related medicine news :

1. Predicting human body height from DNA
2. The human health costs of losing natural systems: Quantifying Earths worth to public health
3. China Human Vaccine Industry Report, 2012-2015
4. Mahendra Trivedi Announces the New Master During the Historical Event “Transforming Humanity”
5. NHS Human Services Foundation to Host 10th Annual Leading the Way Awards Gala
6. Geranylgeraniol suppresses the viability of human prostate cancer cells and HMG CoA reductase
7. Tel Aviv University part of team launching global Human Brain Project
8. Western University of Health Sciences’ Gala Fundraiser Emphasizes Humanism and Caring
9. Animal, human health benefits anticipated from universitys premier biomedical instrument
10. ENT and Allergy Associates, LLP® Adds Resources to Its Human Resources Department, Welcomes Eric M. Saidel, JD as Its New HR Director
11. Human Circuit Completes Town of Vienna’s Council Chambers and Live Cable TV Broadcasting Project
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/2/2016)... ... December 02, 2016 , ... The annual time frame to change Medicare health ... is ending December 7th. Currently-enrolled Medicare beneficiaries who are looking to switch from their ... D) need to make changes during this period order for their new policy to ...
(Date:12/2/2016)... (PRWEB) , ... December 02, 2016 , ... More than ... and while 84 percent of parents report speaking with their child about sex related ... sexually transmitted diseases. , Mediaplanet is proud to announce the launch of its second ...
(Date:12/2/2016)... ... December 02, 2016 , ... Sourced from the Isbre Springs beneath the 5,000 ... unmatched natural purity of just 6 ppm TDS (Total Dissolved Solids) in addition to ... been available in several ShopRite and FoodTown stores in NJ and received rave comments ...
(Date:12/2/2016)... Miami, FL (PRWEB) , ... December 02, 2016 ... ... over 5,100 hot meals to needy individuals and families from eight different sites ... Florida on Thanksgiving Day. Over 1,000 volunteers worked very hard on Thanksgiving morning ...
(Date:11/30/2016)... , ... November 30, 2016 , ... ... they now offer a comprehensive in-house dental plan for all patients. Understanding that ... a plan that gives patients a number of perks, including discounts on many ...
Breaking Medicine News(10 mins):
(Date:12/2/2016)... YORK , December 2, 2016 ... Braces & Support) is Expected to Gain a Significant Market ... to Orthopedic Ailments  ... , According to ... Study on Medical Implants Sterile Packaging: Clamshell Product Type Segment ...
(Date:12/2/2016)... On Thursday, the NASDAQ Composite and ... the Dow Jones Industrial Average managed to stay in green. ... which prompted Stock-callers this morning to look at the performances ... NUVA ), Smith & Nephew PLC (NYSE: SNN ... Cesca Therapeutics Inc. (NASDAQ: KOOL ). You can ...
(Date:12/2/2016)... -- The concept of rare diseases and the idea that ... has been taking shape in Europe ... initiatives related to orphan medicinal products have been emerging at ... states individually. Many member states in the EU have led ... medicinal products, the result of which took the shape of ...
Breaking Medicine Technology: