Navigation Links
Visualization of DNA synthesis in vivo
Date:12/13/2011

Interactions of biological macromolecules are the central bases of living systems. Biological macromolecules are synthesized in living cells by linking many small molecules together. Naturally occurring macromolecules include genetic materials (DNA) and proteins. A detailed understanding of the synthesis of these macromolecules in whole animals is a basic requirement for understanding biological systems, and for the development of new therapeutic strategies.

To visualize the synthesis of biomolecules in living organisms, artificial small molecules can be added to and incorporated by the cell's own biosynthetic machinery. Subsequently, the modified biomolecules containing the artificial units can be selectively labelled with fluorescent substances. Until now, this approach had one major limitation: the substances used for labelling were toxic and caused cell death.

Anne Neef, a PhD student from the Institute of Organic Chemistry at the University of Zurich, has developed a new substance that can replace the natural nucleoside thymidine in DNA biosynthesis. This fluorinated nucleoside called "F-ara-Edu" labels DNA with little or no impact on genome function in living cells and even whole animals. "F-ara-Edu" is less toxic than previously reported compounds used for DNA labelling and it can be detected with greater sensitivity. "F-ara-Edu" is therefore ideally suited for experiments aimed at "birth dating" DNA synthesis in vivo. "As a demonstration of this, F-ara-Edu was injected into Zebrafish eggs immediately after fertilization. Following development and hatching of the fish, the very first cells undergoing differentiation in embryonic development could be identified", explains Anne's research advisor, Prof. Nathan Luedtke. "By visualizing new DNA synthesis in whole animals, the sites of virus infection and cancerous growth can be identified due to the abundance of DNA replication in these tissues", adds Prof. Luedtke. This approach should therefore lead to new strategies in drug development.


'/>"/>

Contact: Nathan Luedtke
luedtke@oci.uzh.ch
41-446-354-244
University of Zurich
Source:Eurekalert  

Related medicine news :

1. Life Coach and Author Thomas Finn's Highly Anticipated Book, 'A Better Tomorrow- Affirmations and Visualizations the Keys to Success,' Debuts June 15
2. Provalis Research Announces the Release of WordStat 6, Delivering Enhanced Analytical and Visualization Tools for Textual Data
3. Sales are on the Rise for Professor McMurphy's Subliminal and Binaural Visualization iPhone Apps
4. Versiera Enables Cloud and Enterprise Network Visualization
5. Hospital gives first tomosynthesis mammograms in region this week
6. A new set of building blocks for simple synthesis of complex molecules
7. Led by advances in chemical synthesis, scientists find natural product shows pain-killing properties
8. Researchers advance biosynthesis of potent anti-cancer drug Taxol
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Visualization of DNA synthesis in vivo
(Date:4/25/2017)... ... April 25, 2017 , ... ... committed to raising awareness for Duchenne muscular dystrophy, and funding for Duchenne ... (cardiosphere-derived cells) Phase I/II HOPE clinical trial in Duchenne announced today. , ...
(Date:4/25/2017)... ... April 25, 2017 , ... Lake Park Dental is now accepting ... Invisalign® in Lutz, FL. With the help of this highly-effective, yet convenient system, ... potential complications, more discretion and less pain. , Drs. Sarah Jockin, Nicole Morganti, ...
(Date:4/25/2017)... ... April 25, 2017 , ... Buyers and sellers in the thriving ... and head shops –can’t help but be heartened by the industry’s current surge. But ... aptly described as “skunk smell.” At last they can simply, safely and effectively ...
(Date:4/25/2017)... ... April 25, 2017 , ... ... healthcare products, is introducing Flexadin UCII, part of the EQUISTRO line, at this ... joint health in horses at the immunologic level. , The scientifically-developed Flexadin UCII ...
(Date:4/24/2017)... ... 24, 2017 , ... The Santana Telehealth Project was honored with the 2017 ... American Telemedicine Association’s annual conference, on April 23 in Orlando, FL. , “I am ... the lives of the poor and underserved in other parts of the world,” said ...
Breaking Medicine News(10 mins):
(Date:4/20/2017)... April 20, 2017  Cogentix Medical, Inc. (NASDAQ: ... providing the Urology, Uro/Gyn and Gynecology markets with ... Keswani has joined the Company as Senior Vice ... created position, Mr. Keswani will report directly to ... "Our organization is delighted that Ash has ...
(Date:4/20/2017)... Mass. , April 20, 2017  RXi ... developing innovative therapeutics that address significant unmet medical ... data from the Company,s consumer product development program, ... at the Society for Investigative Dermatology (SID) 76 ... to advance and promote the sciences relevant to ...
(Date:4/20/2017)... April 20, 2017  AbbVie (NYSE: ABBV), a ... (n=145/146) of chronic hepatitis C virus (HCV) infected ... 6 and compensated cirrhosis (Child-Pugh A) achieved sustained ... ) with its investigational, pan-genotypic regimen of glecaprevir/pibrentasvir ... seen following 12 weeks of G/P treatment without ...
Breaking Medicine Technology: