Navigation Links
Visualization of DNA synthesis in vivo
Date:12/13/2011

Interactions of biological macromolecules are the central bases of living systems. Biological macromolecules are synthesized in living cells by linking many small molecules together. Naturally occurring macromolecules include genetic materials (DNA) and proteins. A detailed understanding of the synthesis of these macromolecules in whole animals is a basic requirement for understanding biological systems, and for the development of new therapeutic strategies.

To visualize the synthesis of biomolecules in living organisms, artificial small molecules can be added to and incorporated by the cell's own biosynthetic machinery. Subsequently, the modified biomolecules containing the artificial units can be selectively labelled with fluorescent substances. Until now, this approach had one major limitation: the substances used for labelling were toxic and caused cell death.

Anne Neef, a PhD student from the Institute of Organic Chemistry at the University of Zurich, has developed a new substance that can replace the natural nucleoside thymidine in DNA biosynthesis. This fluorinated nucleoside called "F-ara-Edu" labels DNA with little or no impact on genome function in living cells and even whole animals. "F-ara-Edu" is less toxic than previously reported compounds used for DNA labelling and it can be detected with greater sensitivity. "F-ara-Edu" is therefore ideally suited for experiments aimed at "birth dating" DNA synthesis in vivo. "As a demonstration of this, F-ara-Edu was injected into Zebrafish eggs immediately after fertilization. Following development and hatching of the fish, the very first cells undergoing differentiation in embryonic development could be identified", explains Anne's research advisor, Prof. Nathan Luedtke. "By visualizing new DNA synthesis in whole animals, the sites of virus infection and cancerous growth can be identified due to the abundance of DNA replication in these tissues", adds Prof. Luedtke. This approach should therefore lead to new strategies in drug development.


'/>"/>

Contact: Nathan Luedtke
luedtke@oci.uzh.ch
41-446-354-244
University of Zurich
Source:Eurekalert  

Related medicine news :

1. Life Coach and Author Thomas Finn's Highly Anticipated Book, 'A Better Tomorrow- Affirmations and Visualizations the Keys to Success,' Debuts June 15
2. Provalis Research Announces the Release of WordStat 6, Delivering Enhanced Analytical and Visualization Tools for Textual Data
3. Sales are on the Rise for Professor McMurphy's Subliminal and Binaural Visualization iPhone Apps
4. Versiera Enables Cloud and Enterprise Network Visualization
5. Hospital gives first tomosynthesis mammograms in region this week
6. A new set of building blocks for simple synthesis of complex molecules
7. Led by advances in chemical synthesis, scientists find natural product shows pain-killing properties
8. Researchers advance biosynthesis of potent anti-cancer drug Taxol
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Visualization of DNA synthesis in vivo
(Date:1/20/2017)... ... ... A new partnership between Goodwill® and Roadie, Inc. aims to make it easier ... couches to dressers and bicycles. Roadie — the national on-the-way delivery network — will ... through February 28th. , “January is an exciting time when resolutions are made ...
(Date:1/20/2017)... FL (PRWEB) , ... January 20, 2017 , ... Source ... botanicals and 100 percent pure essential oils, announced the company had a successful visit ... , The annual ECRM event gives companies that work in the nutritional, sports and ...
(Date:1/20/2017)... (PRWEB) , ... January 20, 2017 , ... Bionic Sports ... all walks of life, announced it had a successful January ECRM Trade Show in ... in the United States, which allows it to provide its products to all clients ...
(Date:1/20/2017)... ... January 20, 2017 , ... ATP Science, an Australian-based company ... attended the January ECRM Trade Show in Hilton Head, SC, benefiting from outstanding ... range of supplements that keep the body functioning at its peak performance by ...
(Date:1/20/2017)... boca raton florida (PRWEB) , ... January 20, 2017 , ... ... trial at the Dana Farber Cancer Institute. For Betsy, the clinical trial has ... and her cancer has not worsened. , Betsy Brauser was diagnosed with ovarian ...
Breaking Medicine News(10 mins):
(Date:1/19/2017)... CITY, Calif. , Jan. 19, 2017  Abaxis, ... company manufacturing point-of-care blood analysis instruments and consumables for ... conference call to discuss its financial results for the ... The call will be at 4:15 p.m. ET on ... results for the third quarter fiscal year 2017 after ...
(Date:1/19/2017)... , Jan. 19, 2017  Sensus Healthcare, Inc. ... company specializing in the treatment of non-melanoma skin ... with superficial radiation therapy, today announced that it ... 2016 financial results on Thursday, February 2, 2017 after ... hold a conference call with the investment community ...
(Date:1/19/2017)... LEXINGTON, Massachusetts , January 19, 2017 ... SHPG ) announced that the U.S. Food and Drug ... resubmission of a New Drug Application (NDA) for SHP465, ... being evaluated as a potential once-daily treatment for Attention-Deficit/Hyperactivity ... decision on or around June 20, 2017, the designated ...
Breaking Medicine Technology: