Navigation Links
Video shows the traffic inside a brain cell
Date:8/22/2012

Using bioluminescent proteins from a jellyfish, a team of scientists has lit up the inside of a neuron, capturing spectacular video footage that shows the movement of proteins throughout the cell.

The video offers a rare peek at how proteins, the brain's building blocks, are directed through neurons to renew its structure. It can be viewed online here: http://www.youtube.com/watch?v=baI9q2--q7s&feature=youtu.be

"Your brain is being disassembled and reassembled every day," said Don Arnold, associate professor of molecular and computational biology at the USC Dornsife College of Letters, Arts and Sciences, and corresponding author of an article about the research that appeared in Cell Reports on July 26.

"One week from today, your brain will be made up of completely different proteins than it is today," Arnold said. "This video shows the process. We've known that it was happening, but now we can watch it happen."

The new imaging technique was used to cast new light on how proteins are directed to one of the two types of compartments inside the neuron: the axon or the dendrites.

The axon is the region of the cell responsible for transmitting electrical signals to other cells, while the dendrites receive signals from other cells.

"It's been known for many decades that proteins are specifically targeted to one compartment or the other. However, we couldn't understand how the targeting occurs until we could actually watch the proteins traveling to one compartment or to the other," said Sarmad Al-Bassam, USC Ph.D. student and lead author of the Cell Reports article.

Since the mid-1990s, scientists have been able to illuminate the proteins inside of cells, including neurons, by attaching a protein isolated from jellyfishknown as GFP (green fluorescent protein)that fluoresces bright green when exposed to blue light.

Martin Chalfie of Columbia University, Roger Tsien of the University of California-San Diego, and Osamu Shimomura of Boston University were awarded the Nobel Prize in Chemistry in 2008 for the discovery and development of GFP.

The problem with studying the flow of illuminated proteins inside of neurons is that there are several different overlapping pathways within the cell, making it difficult to study the traffic through just one.

Al-Bassam and his colleagues solved this issue by developing a new technique that involves damming up a single pathway, which creates a backlog of transport vesicles (little bubbles that travel up and down neurons carrying membrane protein cargo) impregnated with the illuminated proteins. They then use a small-molecule drug to release the backlog all at once in a bright pulse.

"Our result was very surprising," said Don Arnold, associate professor of molecular and computational biology at the USC Dornsife College of Letters, Arts and Sciences and corresponding author of the Cell Reports article. "We found that rather than being targeted specifically to the dendrites, vesicles carrying proteins initially enter both compartments, but then are stopped and prevented from moving beyond the initial segment of the axon."


'/>"/>

Contact: Robert Perkins
perkinsr@usc.edu
213-740-9226
University of Southern California
Source:Eurekalert

Related medicine news :

1. Sexual objectification of female artists in music videos exists regardless of race, MU study finds
2. Animated characters keep it real in teen violence prevention videos
3. Study finds that patient education videos viewed before an operation may benefit patients
4. Video-assisted thoracic surgery valuable tool in lung cancer screening
5. New American Chemical Society video: Behind the scenes tour of an electronic nose lab
6. New Report Video Surveillance Market (2011 – 2016) by MarketsandMarkets
7. AuntMinnie Begins Live Video Stream of ISCT Conference Proceedings
8. Repelling the rays: New American Chemical Society video on sunscreens
9. USA Payday Forever Announces New Payday Loan Training Videos For Customers
10. CustomerCentric Selling® Unveils New Client Testimonial Videos to Showcase Significant Sales Success
11. Content Writing King Announces Cancellation Of Experimental Video Creation Process For Their Article Marketing Website
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2016)... ... June 24, 2016 , ... Global law firm Greenberg Traurig, ... Elite. The attorneys chosen by their peers for this recognition are considered among the ... Traurig Shareholders received special honors as members of this year’s Legal Elite Hall of ...
(Date:6/24/2016)... ... 2016 , ... Comfort Keepers® of San Diego, CA is excited to announce ... program to drive cancer patients to and from their cancer treatments. Comfort Keepers ... of life and ongoing independence. Getting to and from medical treatments is one ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... People across ... in Genome magazine’s Code Talker Award, an essay contest in which patients and their ... award to be presented at the 2016 National Society of Genetic Counselors (NSGC) Annual ...
(Date:6/24/2016)... ... June 24, 2016 , ... The Haute Beauty Network, ... M. Weintraub as a prominent plastic surgeon and the network’s newest partner. ... the most handsome men, look naturally attractive. Plastic surgery should be invisible.” He ...
(Date:6/24/2016)... Frederick, Maryland (PRWEB) , ... June 24, 2016 ... ... Mid-Atlantic Angels is actively feeding the Frederick area economy by obtaining investment capital ... support over the past 2½ years that have already resulted in more than ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... startling report released today, National Safety Council research shows ... plan to eliminate prescription opioid overdoses. Prescription Nation ... the worst drug crisis in recorded U.S. history, assigned a "Making ... , New Mexico , Tennessee ... states, three – Michigan , Missouri ...
(Date:6/23/2016)... Research and Markets has announced the addition of ... Forecast to 2022" report to their offering. ... date financial data derived from varied research sources to present ... impact on the market during the next five years, including ... sub markets, regional and country level analysis. The report provides ...
(Date:6/23/2016)... Research and Markets has announced the addition of the ... offering. The current unmet ... for MedImmune to enter. The US ageing population creates a ... considerable growth for effective anti-influenza medications. The introduction of a ... development is still in its infancy. Key ...
Breaking Medicine Technology: