Navigation Links
Vaporized viral vector shows promise in anti-cancer gene therapy

A new lung cancer therapy employing a vaporized viral vector to deliver a cancer-inhibiting molecule directly to lung tissue shows early promise in mouse trials, according to researchers at the Ministry of Education, Science and Technology in Korea.

Gene therapy is an area of great promise, but delivery mechanisms, which have included intravenous injection and intratracheal instillation, have proven problematic for effective delivery of genetic therapy to lung tissues.

"Aerosol delivery targets the lungs specifically and represents a noninvasive alternative for targeting genes to the lung," wrote Myung-Haing Cho, D.V.M., Ph.D., professor at Seoul National University and principal investigator of the study. "The delivery of genes via aerosol holds promise for the treatment of a broad spectrum of pulmonary disorders and offers numerous advantages over more invasive modes of delivery."

The results of Dr. Cho's promising research will be published in the June 15 issue of the American Journal of Respiratory and Critical Care Medicine.

Lung cancer is the most common cause of cancer deaths worldwide, killing more people each year than breast, prostate and colon cancers combined. It costs the U.S. alone more than $9 billion a year, according to the Centers for Disease Control and Prevention. Most available therapiessurgery, radiation and chemotherapyoffer transient relief at best and are typically ineffective in advanced stages of the disease. For this reason, novel therapies for lung cancer are of great interest.

Dr. Cho and colleagues targeted the Akt signaling pathway, which has been shown to be an important regulator of cell proliferation and cancer progression. A recent report found that 90 percent of non-small cell lung carcinomas were associated with the activation of the Akt signaling pathway. They chose a lentiviral vector, derived from a retrovirus and known for its ability to infect nondividing cells and effect persistent genetic changes. They transfected the lentiviral vector with a negative regulator of Akt signaling, carboxyl-terminal modulator protein (CTMP), which would theoretically inhibit Akt signaling, thus suppressing cancer cell proliferation and tumor growth.

Using a mouse model of lung cancer, the researchers designed a double-control study, exposing one-third of the mice to the aerosolized CTMP vector, one-third to the vector alone and one third were untreated.

"In this study, our main purpose was to determine if viral delivery of CTMP can provide useful tool for designing lung tumor treatment," said Dr. Cho. "We would like to demonstrate that CTMP can suppress lung tumor mass in the lungs and lentivirus may act as an effective carrier of CTMP."

After four weeks of twice-weekly treatments, the researchers found exactly that: both pathological and histological examination of the mice revealed that CTMP delivery suppressed lung tumor mass in the lungs of the mice. Furthermore, the number and volume of tumors were significantly decreased in CTMP-treated mice.

The researchers also found that CTMP increased apoptosis, inhibited angiogenesis and suppressed production of several proteins, such as cyclin D1, CDK4 and CDK2, which are important in cancer cell growth.

"Our results demonstrated that lentivirus-mediated CTMP overexpression suppressed Akt activity and inhibited tumor progression," wrote Dr. Cho. "Repeated aerosol gene delivery may provide an effective noninvasive model of gene delivery and understanding the role of CTMP in the multistage lung tumorigenesis may be essential in developing effective therapeutics for lung cancer."


Contact: Keely Savoie
American Thoracic Society

Related medicine news :

1. Pill box organizers increase HIV patients adherence and improve viral suppression
2. DNA damage response confers a barrier for viral tumorigenesis
3. Key Viral Change Could Help Bird Flu Spread
4. Antiviral Wont Improve Bells Palsy Outcome
5. Mayo Clinic Proceedings article explores possible link between obesity and viral infections
6. Is it pancreatitis in acute abdominal pain in acute viral hepatitis?
7. American Medical Association Clears Path for Adenoviral Conjunctivitis - Pink Eye - Detector
8. Antivirals reduce deaths from flu in hospitalized patients
9. MIGENIX Reports Preliminary Celgosivir Viral Kinetics Study
10. Antiretroviral Drugs May Prevent Vaginal Transmission of HIV
11. IPM Reaches Landmark Agreement with Pfizer to Develop FDA-Approved Antiretroviral Drug as Vaginal Microbicide
Post Your Comments:
(Date:10/13/2015)... ... , ... Sir Grout of Greater Boston donated an expansive, seven room ... House Charities® (RMHC®). This donation was made in an effort to give back to ... inception. , “We believe strongly in the Ronald McDonald House Charities, and they are ...
(Date:10/13/2015)... (PRWEB) , ... October 13, 2015 , ... ... its newest technology platform, ENGAGE, at HIMSS’s Patient Engagement Summit . HealthAware ... , ENGAGE delivers innovative health programs and interventions via mobile devices that provide ...
(Date:10/13/2015)... ... October 13, 2015 , ... Element ... products, introduced a new company, RightSensor™ LLC, an Internet of Things (IoT) hardware ... RightSensor™ provides a fully-managed approach for customers requiring sensor hardware for critical data ...
(Date:10/13/2015)... ... , ... NavaFit Inc. today announced the launch of its NavaFit app for ... local fitness & sporting events, and stay motivated. Users can download the ... us to get more serious about fitness and wellness, individuals are constantly looking for ...
(Date:10/13/2015)... , ... October 13, 2015 ... ... Omega-3, a first-of-its kind product that targets the unique health needs of ... of the American Pregnancy Association ( ), utilizes Nordic Naturals’ exclusive, ...
Breaking Medicine News(10 mins):
(Date:10/13/2015)... , Oct. 13, 2015  A minimally-invasive treatment just ... the United States.  After more than 10 years of ... 9 approved the use of High Intensity Focused Ultrasound ... cells while protecting surrounding tissue and minimizing chances for ... George Suarez , a pioneering Miami ...
(Date:10/13/2015)... N.J. , Oct. 13, 2015  ContraVir ... the "Company"), a biopharmaceutical company focused on the ... announced the closing of its previously announced underwritten ... stock and warrants to purchase up to 3,000,000 ... fixed combined price to the public of $3.00. ...
(Date:10/13/2015)... , Oct. 13, 2015  Measurement in accountable ... and balancing financial incentives, but gaps in measurement ... care and health systems. A new, peer-reviewed ... Managed Care explores measurement gaps for ... sets. --> --> ...
Breaking Medicine Technology: