Navigation Links
Unexpectedly small effects of mutations in bacteria bring new perspectives
Date:11/5/2010

Most mutations in the genes of the Salmonella bacterium have a surprisingly small negative impact on bacterial fitness. And this is the case regardless whether they lead to changes in the bacterial proteins or not. This is shown by Uppsala University scientists in an article being published today in the prestigious journal Science.

The researchers have examined the impact of mutations on the rate of growth of the Salmonella bacterium and show that most mutations have generally very small effects. Moreover the negative effects are of the similar magnitude for changes that lead to substitution of amino acids in proteins (so-called non-synonymous mutations) as for mutations that do not change the protein sequence (so-called synonymous mutations).

"The findings open an entirely new chapter for experimental studies of mutations and show that we need to change our view of how mutations lead to negative effects," says Professor Dan Andersson, lead author of the study.

A central question in evolutionary biology, medical genetics, species-conservation biology, and animal breeding is how and why mutations affect an organism's capacity to survive. Usually these questions are studied in DNA sequence analyses from which conclusions have been drawn about what mutations are most common and have become established in the DNA of the organism.

The Uppsala scientists have used another -- experimental -- method whereby they can use various genetic tricks to introduce random individual mutations into any chosen gene, a method that has previously been used primarily in viruses. Two genes that code for proteins that are included in ribosomes were mutated, and using extremely sensitive growth measurements, doctoral candidate Peter Lind showed that most mutations reduced the rate of growth of bacteria by only 0.500 percent. No mutations completely disabled the function of the proteins, and very few had no impact at all.

Even more surprising was the fact that mutations that do not change the protein sequence had negative effects similar to those of mutations that led to substitution of amino acids. A possible explanation is that most mutations may have their negative effect by altering mRNA structure, not proteins, as is commonly assumed.


'/>"/>

Contact: Dan Andersson
Dan.Andersson@imbim.uu.se
46-018-471-4175
Uppsala University
Source:Eurekalert

Related medicine news :

1. UCI non-small cell lung cancer study highlights advances in targeted drug therapy
2. Small particles show big promise in beating unpleasant odors
3. Small business success spotlighted by USDA at conference
4. American Chemical Society Webinar focuses on NSFs small business funding programs
5. Health Care Reform Helps Small Businesses Offer Coverage: Report
6. 16.6 million small business employees could benefit from ACA provisions starting this year
7. Babies Born Late May Face Small Cerebral Palsy Risk
8. Small Amounts of Dark Chocolate May Guard Against Heart Failure
9. Henry Ford Hospital study: Donor Risk Index does not impact outcomes on a small scale
10. Small increases in vaccine cost can cause large gaps in protection
11. Smaller hospitals can provide safe and high-quality surgical care comparable to larger counterparts
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/27/2016)... ... May 27, 2016 , ... Each year ... complementary medicine. Allison Outerbridge is this year’s Life University winner of ... at the university’s Student Leadership Awards ceremony. , Outerbridge is approaching her last ...
(Date:5/26/2016)... ... 26, 2016 , ... W.S. Badger Co. Inc ., the maker of ... When Work Works Award for its use of effective workplace strategies to increase business ... project administered by the Families and Work Institute (FWI) and the Society for Human ...
(Date:5/26/2016)... ... May 26, 2016 , ... Connor Sports, ... as a partner for the Tamika Catchings Legacy Tour that will commemorate ... leader in hardwood basketball surfaces in all forms and levels of the game, Connor ...
(Date:5/26/2016)... ... May 26, 2016 , ... There are nearly 14.5 million ... million cancer survivors worldwide. On Sunday, June 5, 2016, communities around the world will ... Survivors Day®. , National Cancer Survivors Day® is an annual worldwide Celebration of ...
(Date:5/26/2016)... ... May 26, 2016 , ... An April Gallup survey found ... The 550 employees of Sun Health Senior Living (SHSL) may not share ... their doctor and prescription copays for the year, while holding the line on increasing ...
Breaking Medicine News(10 mins):
(Date:5/24/2016)... LONDON , May 24, 2016 ... erfüllt beide primären Endpunkte ... und Überlegenheit in ‚ausgezeichneter plus guter ... aufsteigenden Colons    ,      (Logo: ... B.V. gab heute neue positive Daten von der ...
(Date:5/24/2016)... Mass. , May 24, 2016  NxStage Medical, ... technology company focused on advancing renal care, today announced ... plans to participate in the following schedule of investor ... be made available at http://ir.nxstage.com/ . ... Jefferies Healthcare Conference NY, NY           Friday, June ...
(Date:5/24/2016)... HONG KONG , May 24, 2016 /PRNewswire/ ... primer stent de doble terapia del mundo, introduce ... fístula arteriovenosa. OrbusNeich, una compañía global ... cambian las vidas, ha expandido su cartera incluyendo ... catéteres balón JADE™ y Scoreflex™ PTA son los ...
Breaking Medicine Technology: