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Ultrasound could boost tissue implant success
Date:5/27/2010

rsts of three or ten minutes. They assayed for the number of cells, metabolism (by observing how much glucose they consumed and how much lactate they produced), viability and for signs of damage to the cells.

The C2C12 muscle cells stimulated with LIUS showed greater cell numbers and better viability than controls. Also for the first time the researchers obtained preliminary evidence that LIUS can influence the viability of the cultured adipose cells (known as organoids) in an in vitro organ culture model. Adipose tissue treated with LIUS showed significantly increased metabolic activity, and had fewer markers for tissue damage than tissue not treated with LIUS.

If the technique was used on a patient, the way that ultrasound might enhance molecular motion would probably depend on local variations in tissue density. "Depending on the location of the probe, one can expect variable effects of LIUS," says senior author Steven Zeitels, MD, Director of the Center for Laryngeal Surgery and Voice Rehabilitation.

It's also not clear whether the increased metabolic activity and proliferation of the cells seen in this experiment was simply due to LIUS's mechanical and thermal effect in stimulating molecules to move around more. "In the context of using LIUS to enhance autograft survival, the possibility that the LIUS can directly activate signalling pathways in implanted cells needs to be taken into account. It may eventually be possible to manipulate cellular responses by fine-tuning this technique." says lead author Hyoungshin Park, PhD, from MIT.

It remains to be seen whether these laboratory results will hold up in in vivo studies, but these preliminary results suggest important avenues to pursue in efforts to improve graft survival.


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Contact: Mithu Lucraft
mithu.lucraft@sagepub.co.uk
44-207-324-2223
SAGE Publications UK
Source:Eurekalert

Page: 1 2

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