Navigation Links
UV light controls antibodies, improves biosensors
Date:10/31/2011

WASHINGTON, Oct. 31From detecting pathogens in blood samples to the study of protein synthesis, Quartz Crystal Microbalance (QCM) sensors have many uses in modern biology. In this technique, antibodies anchored to gold electrodes on a piece of quartz crystal act like the "hooks" on the sticky side of a Velcro strap, grabbing molecules of interest as they pass by. The more molecule-sensing antibodies on the surface of the sensor, the more sensitive the QCM device's detection capabilities.

Unfortunately, some of the antibodies typically anchor themselves to the gold plate "hook"-side-down, rendering them useless as bio-receptors and dampening the sensor's sensitivity. Now researchers from the University of Naples "Federico II" and the Second University of Naples in Italy have found a way to increase the number of right-side-up antibodies in this well-established molecule detection process using light. In a paper recently published in the Optical Society's open-access journal Biomedical Optics Express, the team of scientists irradiated antibodies with ultra-short pulses of ultraviolet (UV) light. The UV light is absorbed by the amino acid tryptophan, which breaks the disulfide bridges holding parts of the antibody together and causes a particular part of the amino acid cysteine, called a thiol group, to become exposed at the tail end of the antibody. Because thiol groups are more strongly attracted to the gold electrodes than other parts of the antibody, the bottom sides of these irradiated antibodies become much more likely to adhere to the gold electrodes than the "hook" ends. Using this method, the researchers were able to more than double the sensitivity of the QCM device, opening up new possibilities for research using this type of sensor, the researchers say.


'/>"/>

Contact: Angela Stark
astark@osa.org
202-416-1443
Optical Society of America
Source:Eurekalert  

Page: 1

Related medicine news :

1. Research highlights training to improve colorectal cancer detection
2. Python Findings Shed Light on Human Heart Health
3. High-quality white light produced by four-color laser source
4. Genes Could Highlight Plavix Users at Clot Risk After Stent
5. CHEST 2011: Embargoed studies highlight new lung cancer and COPD research
6. Study highlights issues faced by friends and family of the suicidal
7. Innovations in minimally invasive surgery and education are highlights of 40th AAGL Global Congress
8. Malaria elimination maps highlight progress and prospects
9. Research Sheds Light on Vitamin Ds Role in Immunity
10. Even Slightly Higher Blood Pressure May Boost Stroke Risk
11. APS Division of Fluid Dynamics meeting: Highlights and media registration
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
UV light controls antibodies, improves biosensors
(Date:4/27/2017)... ... April 27, 2017 , ... Plastic Surgery Associates is excited to ... to present at the upcoming Aesthetic Meeting. Held in San Diego, at the San ... and Canales will lend their expertise to the Premier Global Hot Topics session, speaking ...
(Date:4/26/2017)... ... 26, 2017 , ... CareSet Labs today is releasing an ... in Part D Medicare data. The dataset, called PaPR (Providers and Prescribing Records) ... or paper. The PaPR (pronounced "paper") data set aggregates this information on ...
(Date:4/26/2017)... ... April 26, 2017 , ... Miami native and local ... , “I could have never imagined back in 1991 that this journey would have ... , Over the last 25 years, Dr. Gallardo has pioneered implant dentistry innovation, evolving ...
(Date:4/26/2017)... ... April 26, 2017 , ... ... is proud to announce that it has received 510(k) clearance from the U.S. ... Home and the MyoCycle Pro. , Both devices are stationary cycling systems that ...
(Date:4/25/2017)... (PRWEB) , ... April 25, 2017 , ... A stressful ... at work can also decrease overall productivity and performance in the workplace. The goal ... , During the last few weeks of April, Clearview Resolution Services will be shutting ...
Breaking Medicine News(10 mins):
(Date:4/19/2017)... 19, 2017 The Global Effective Microorganisms ... 2022 report has covered and analysed the potential of Global ... market size, shares and growth factors. The report identifies and ... opportunities in the global market. ... Browse 152 Tables and Figures, 6 Major Company Profiles, ...
(Date:4/19/2017)... , April 19, 2017 ... an immune response in pets such as canine, ... are of various types such as Attenuated Live ... Vaccines, DNA Vaccines and Recombinant Vaccines. Attenuated live ... virus or bacteria, which have been weakend under ...
(Date:4/19/2017)... N.J. , April 19, 2017  Novartis ... by the National Heart, Lung, and Blood Institute ... demonstrating that 58% of patients with treatment-naïve severe ... months when treated with eltrombopag at the initiation ... . The study evaluated three sequential treatment groups, ...
Breaking Medicine Technology: