Navigation Links
UD team develops new method for producing proteins critical to medical research
Date:4/1/2010

Scientists at the University of Delaware have developed a new method for producing proteins critical to research on cancer, Alzheimer's, and other diseases.

Developed by Zhihao Zhuang, UD assistant professor of chemistry and biochemistry, and his research group, the chemical method yields hundredsfold more ubiquitylated proteins than current approaches. Such proteins may hold the key to revealing such mysteries as how cancer cells gain resistance to cancer drugs.

The advance is reported in the April issue of Nature Chemical Biology, the leading journal in the field of chemical biology. Zhuang's co-authors include graduate students Junjun Chen and Jialiang Wang and postdoctoral fellow Yongxing Ai, all from UD, and Lajos Haracska, a researcher in the Institute of Genetics at the Hungarian Academy of Sciences.

Ubiquitin is a small protein, the basis of Nobel Prize-winning research in 2004, which deemed the molecule the "kiss of death" for its role in tagging damaged or unneeded proteins for the cell's waste disposal in the constant process of protein generation and degradation. In recent years, the non-proteolytic functions of ubiquitin in diverse cellular processes, including protein trafficking, immune response, and DNA damage tolerance, have been discovered at a rapid pace, and it has become clear that ubiquitin plays far-broader roles in cell biology.

However, preparing sufficient samples of ubiquitylated proteins for study has been a major challenge facing scientists.

The availability of these proteins is critical for Zhuang and members of his research team, who are working at the interface of chemistry and biology trying to understand the molecular basis of human cancer development and prevention.

The new method for developing ubiquitylated proteins, which Zhuang and his team developed, combines the power of intein chemistry and disulfide crosslinking to bond ubiquitin to another essential protein called proliferating cell nuclear antigen.

"Our yield is hundredsfold higher compared to the commonly used enzymatic approach," Zhuang says. "We also have the flexibility of modifying the selected residues, which has not been possible with the previous approach."

In investigating the effect of the differently modified proteins, Zhuang and his group also revealed a surprising phenomenon regarding ubiquitylation.

"We found that ubiquitin as a protein modifier is far more flexible than we have thought. This property distinguishes ubiquitylation from other better studied protein post-translational modifications, such as phosphorylation and acetylation," Zhuang says.

The new UD approach will help researchers studying ubiquitin biology by providing the means to prepare milligrams of protein samples for in-depth structural and functional characterization.

SInce the publication of the work online in Nature Chemical Biology, Zhuang has received requests for samples from research groups across the United States.

Additionally, the new approach has already opened up doors to new research in Zhuang's own laboratory, where he and his team are investigating new anti-cancer therapies.


'/>"/>

Contact: Tracey Bryant
tbryant@udel.edu
302-831-8185
University of Delaware
Source:Eurekalert  

Related medicine news :

1. New York City Surgeon Develops All-Natural Treatment for Wrinkles and Scars
2. Orlando Company Develops New Technology Designed To Help Combat Hospital-Acquired Infections
3. Nu-Tek Products Develops Cultured Soymilk Powder
4. Neuroimaging may shed light on how Alzheimers disease develops
5. Personal Best Develops Workplace Weight Loss Program
6. M. D. Anderson develops tool to measure severity of chronic graft-vs.-host disease symptoms
7. Study Finds Ponseti Method of Clubfoot Correction Leads to Lower Surgical Rates and Less Revision Surgery
8. Doctors and Scientists Create New Society to Promote Non-Animal Toxicology Testing Methods
9. Standard Methods Miss 50% of Pharmaceutical Key Opinion Leaders
10. New Web-Based MethodOne Clinic Management Software
11. Researchers discover method to objectively identify PTSD
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
UD team develops new method for producing proteins critical to medical research
(Date:2/5/2016)... , ... February 05, 2016 , ... At its annual ... Patrick McDermott as Chairman of the National Board of Directors. Mr. McDermott succeeds former ... the Board,” stated Leslie A. Chambers , APDA President and CEO. “Pat has ...
(Date:2/5/2016)... ... 05, 2016 , ... Dr. Ben Amini, a San ... welcoming orthodontist, Dr. Amanda Cheng, to the practice. With the addition of services ... including general dentistry, cosmetic treatments, periodontics, implant dentistry , endodontics, orthodontics and ...
(Date:2/5/2016)... ... February 05, 2016 , ... Health and wellness is a topic ... in the event they are experiencing an illness. Migraines are a severe form of ... afflicted with migraines would not wish the pain on their worst enemy, the feeling ...
(Date:2/5/2016)... , ... February 05, 2016 , ... Calls Blacklist has ... completely new user interface design and the developer has fixed known bugs within the ... want to on their phone while not consuming any of their device’s battery power ...
(Date:2/5/2016)... ... February 05, 2016 , ... Susan Polk Health Insurance Company recently ... expect to see in 2016. To view the report, see below, or visit ... many San Luis Obispo seniors who rely on Medicare health insurance and whose monthly ...
Breaking Medicine News(10 mins):
(Date:2/5/2016)... , Feb. 5, 2016 ... addition of the "Global Obstetrics Partnering ... company profile to their offering. --> ... of the "Global Obstetrics Partnering 2010-2016: ... profile to their offering. --> ...
(Date:2/4/2016)... 2016 Worldwide Radiology Oncology surgical robot ... next generation systems provide a way to improve ... surgery. New systems pinpoint the delivery of radiation ... such a problem previously, limiting the quantity of ... cancer surgery far beyond what has been available, ...
(Date:2/4/2016)... -- Montoya Love is recognized by Continental Who,s Who as ... is the Regulatory Systems Operations Manager with Becton ... Manufacturing and selling a broad range of medical ... institutions, clinical laboratories and life science researchers with the ... An ...
Breaking Medicine Technology: