Navigation Links
UCSF creates fast, affordable tool for finding gene 'on-off' switches
Date:5/19/2009

UCSF scientists have created a method of quickly identifying large numbers of the genetic material known as short hairpin RNA also called shRNA that turns genes on and off.

The method, which the research team has used to create a library of 22,000 of these "on-off" switches for roughly 600 human genes, provides a tool for scientists throughout the world, with ultimate impacts on the study of the genetic basis of diseases ranging from HIV to high cholesterol, Parkinson's disease or cancer. The team is currently working on creating an shRNA library for the entire human genome.

Findings are available in the Advanced Online Publication section of the journal Nature Methods at http://dx.doi.org/10.1038/nmeth.1330 and will appear in the June 2009 print issue of the journal.

Since their discovery in the past decade, biologically active small RNAs have become the holy grail of much pharmaceutical and genomics research, offering the hope of creating targeted therapeutics to "turn off" or turn down the "volume" of various genes involved in disease, according to Michael McManus, PhD, a UCSF microbiology professor and senior author on the paper. This process is known as RNA interference.

Until now, however, the process of finding shRNAs that are able to potently deactivate a given gene has been extremely laborious and laden with many false results.

The new method uses a microarray technology to generate large numbers of these shRNA strands for each target gene roughly 10 times the volume of traditional methods thereby enabling researchers to screen for RNA interference much faster than before and at a minimal cost, McManus said. "This means that even a small laboratory with limited resources can perform complex genetic screens on mammalian cell cultures," he said. "With the current scarcity of research funding, these are invaluable tools for researchers worldwide
'/>"/>

Contact: Kristen Bole
kbole@pubaff.ucsf.edu
415-476-2557
University of California - San Francisco
Source:Eurekalert

Page: 1 2

Related medicine news :

1. S.C. BlueCross Creates Corporate Planning Unit, Promotes Davis to Vice President
2. TriWest Creates First-of-its-Kind Partnership to Offer Military Leaders With Grief Support Program
3. Exceptional Childrens Foundation Merger with Kayne Eras Center Creates One of States Most Comprehensive Nonprofits Serving the Disabled
4. Prime Access Creates Groundbreaking Ads Targeting Urban Teens for the White Houses National Youth Anti-Drug Media Campaign
5. L.A. Playwright Creates an Enormous Wave with His New Book, Touch the Ocean
6. Alter+Care Creates the Advisory Board
7. Hollings Cancer Center Creates $1 Million Endowed Chair for Breast Cancer Research and Treatment with Gift from the Spaulding-Paolozzi Foundation
8. Bayer Foundation Grant Creates Graduate Engineering Fellowship at University of Houston
9. Creative Technology Services Creates a New Operating Company Focused on Mobility
10. inVentiv Health Creates Client Development and Integration Team
11. National Heart Lung and Blood Institute creates national network to study cardiovascular disease
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/22/2014)... (PRWEB) August 23, 2014 2014 ... is a professional and in-depth research report on ... Glucose Meter information, including Glucose Meter definition, classification, ... industry overview. This research covers the international market ... industry analysis covering macroeconomic environment & economic situation ...
(Date:8/22/2014)... Today, Zane Benefits, the #1 Online ... Health Plans. , According to Zane Benefits, employers are ... lower the cost of healthcare without reducing coverage for ... however, can be confusing. , The new guide contains ... main types of ABHPs. It also explains the ...
(Date:8/22/2014)... NV (PRWEB) August 22, 2014 ... that is written by marketing experts for marketers to ... links has caught the attention of Shane Michaels, prompting ... with Internet marketing in any way knows that content ... in the industry,” reports Michaels. “But even though content ...
(Date:8/22/2014)... Sacramento, CA (PRWEB) August 22, 2014 Buying ... to an adult who is unsure about available coverage types ... now providing principal life insurance policy pricing to the public ... The use of this tool is free and is expected ... are available to the public throughout the U.S. The system ...
(Date:8/22/2014)... 22, 2014 Millennium Treatment Group has recently ... a high level of drug use among teenagers. The treatment ... drug addiction and work to minimize drug abuse. , America ... and drug addiction in history. According to the National ... million Americans aged 12 or older—or 9.2 percent of the ...
Breaking Medicine News(10 mins):Health News:Glucose Meter Industry Global (Europe, Asia, NA, ROW) Analysis Now Available at ReportsnReports.com 2Health News:Glucose Meter Industry Global (Europe, Asia, NA, ROW) Analysis Now Available at ReportsnReports.com 3Health News:My Writers: Review Exposes Online Source for Quality Marketing Content 2Health News:Principal Life Insurance Policies Now Quoted for Adults at Insurance Website Online 2Health News:Millennium Treatment Group Sees Increase in Marijuana Use Among Youth 2
... a 27% Increase in Revenues, DALLAS, Oct. 30 ... of Internet-based claims,processing solutions for the healthcare industry, today ... ended September 30, 2008., "We are pleased to ... Don Crosbie, chief executive officer of Claimsnet.,"The increase in ...
... Philips,Electronics (NYSE: PHG , AEX: PHI) today ... offer quick relief from seasonal mood-related,problems. With the ... affected,by the increased darkness and experience what is ... blues may include lethargy, lowered mood, problems,sleeping, carbohydrate ...
... Mylan Inc. (NYSE: MYL ),today announced that ... company has declared its quarterly dividend of $16.25 per,share ... a liquidation,preference of $1,000.00 per share) payable on Nov. ... of Oct. 31, 2008., Mylan Inc., which provides ...
... VIRGINIA BEACH, Va., Oct. 30, 2008 AMERIGROUP,Corporation (NYSE: ... in the,17th Annual Credit Suisse Healthcare Conference on November ... Company,s presentation, which begins Thursday,November 13 at 4:00 p.m. ... http://www.amerigroupcorp.com, on the investors, page. A replay will,be ...
... age of disease onset and nerve cell death , , ... genes may be linked to the most common form ... team, from Massachusetts General Hospital-MassGeneral Institute for Neurological Disease ... families with a history of the devastating neurological disorder. ...
... Oct. 30 WellPoint,s commercial marketing,team was awarded ... Association,(DMA) for its innovative campaign, "Ecosphere -- Simple ... International ECHO Awards competition is the only international,award ... creativity,strategy and response results., WellPoint,s campaign, conducted ...
Cached Medicine News:Health News:Claimsnet.com Reports Third Quarter 2008 Results 2Health News:Claimsnet.com Reports Third Quarter 2008 Results 3Health News:Philips Introduces New, Advanced Light Therapy Device to Offer Quick Relief from Winter Blues 2Health News:Philips Introduces New, Advanced Light Therapy Device to Offer Quick Relief from Winter Blues 3Health News:Scientists Spot 4 New Alzheimer's Genes 2Health News:WellPoint Awarded Prestigious International Marketing Award 2
(Date:8/22/2014)... Aug. 22, 2014  Mark Riccio, director of ... facility, is using the online crowd-funding platform Kickstarter ... imaging to researchers and would-be superheros everywhere: "for ... would YOU do if you had Superman,s X-ray ... and explorers everywhere, from elementary through high school ...
(Date:8/22/2014)...  WaferGen Bio-systems, Inc. (NASDAQ: WGBS) today announced the ... common stock and warrants at a price of $10,000 ... to deducting underwriting discounts and commissions and offering expenses ... immediately separable and will be issued separately.  The shares ... The NASDAQ Stock Market under the ticker symbol "WGBS" ...
(Date:8/21/2014)... , Aug. 21, 2014  eRelevance Corporation ... company, announced today that it has completed integration ... solution for Plastic Surgery and Dermatology practices. ... more value to healthcare practices using Nextech," stated ... eRelevance Corporation. "Using information from the EMR we ...
Breaking Medicine Technology:Cornell's X-ray Imaging Lab Director Uses Kickstarter Campaign to "Democratize" Advanced 3D Imaging 2WaferGen Bio-Systems Announces Pricing of $20 Million Underwritten Offering and Uplisting to NASDAQ 2WaferGen Bio-Systems Announces Pricing of $20 Million Underwritten Offering and Uplisting to NASDAQ 3WaferGen Bio-Systems Announces Pricing of $20 Million Underwritten Offering and Uplisting to NASDAQ 4eRelevance Corporation Integrates with Nextech Electronic Medical Records 2
... WARREN, N.J., Jan. 17, 2012 Roka Bioscience announced ... AOAC Performance Tested Method(SM) certification from the ... was conducted by Silliker, Inc., as part of the ... The Roka Listeria Detection Assay runs on the ...
... Positron Corporation (OTCBB: POSC), a leading molecular imaging company ... to announce the acquisition of Manhattan Isotope Technology, LLC ... operations and retained all employees of MIT. In exchange, ... and earn-out consideration of $3,500,000 based on 20 percent ...
Cached Medicine Technology:Roka Bioscience Awarded AOAC-RI Certification for Roka Listeria Detection Assay 2Positron Finalizes Acquisition of Manhattan Isotope Technology, LLC 2Positron Finalizes Acquisition of Manhattan Isotope Technology, LLC 3
... ergonomic chairs with concave seats and large ... like seats, backrests, armrests, and casters and ... top performance in a variety of environments ... control, health care and office. • With ...
Contoured waterfall-front ergonomic seats and generous backrests with lumbar support are standard on these ergonomic chairs. • With casters • With Tubular Base....
Contoured waterfall-front ergonomic seats and generous backrests with lumbar support are standard on these ergonomic chairs. • With casters • With Tubular Base....
Contoured waterfall-front ergonomic seats and generous backrests with lumbar support are standard on these ergonomic chairs. • With Tubular Base....
Medicine Products: