Navigation Links
UCSD study reveals the regulatory mechanism of key enzyme
Date:9/21/2007

Research conducted at the University of California, San Diego (UCSD) School of Medicine has shed new light on the structure and function of one of the key proteins in all mammalian cells, protein kinase A (PKA), an enzyme which plays an essential role in memory formation, communication between nerve cells, and cardiac function.

Utilizing a process called x-ray crystallography, the scientists solved the structure of the large PKA complex, revealing a totally new structure that shows PKAs amazing ability to function as a scaffold, that supports and controls the release of chemicals involved in transmitting signals. The structure is shown in the September 21 issue of the journal Cell, featuring the study that describes the dynamic regulatory subunit of PKA.

PKA belongs to a large superfamily of proteins whose activity is regulated by an important small molecule, cyclic AMP (cAMP), in the cell. Protein kinases transmit chemical signals within the cell to regulate a host of functions, such as cell growth or metabolism. Certain protein kinases have been implicated in the uncontrolled growth of cells; for example, when PKA somehow stays on, its prolonged activation can lead to cardiac disease and breast cancer.

By revealing its highly accurate three-dimensional structure, the UCSD scientists have shown how PKA is inhibited and activated by cAMP. PKA contains two components, the regulatory and catalytic subunits. When the subunits are together in the absence of cAMP, the signaling is turned off; when the two parts break apart after being activated by cAMP, PKA is turned on.

We knew how the two subunits, the catalytic and regulatory subunits, looked as separate entities. But we didnt understand how they actually fit together and are activated by cAMP until we saw this structure, said Susan Taylor, Ph.D., Howard Hughes Medical Institute Investor and professor of pharmacology at UCSD School of Medicine, who headed the study.

Discovery of this enzymes molecular structure may help researchers to design drugs that specifically block the protein kinase activity involved in cancer or cardiac disease.

Scientists didnt really understand how the structure unfolded before now, said Taylor, adding that preventing the subunits from coming apart may be an effective way to inhibit diseases caused when PKA is activated and cant turn itself off. Taylor said the researchers were surprised at how much the structure changed when PKA is turned off. The regulatory subunit opens up and literally wraps itself around the catalytic subunit, thus completely turning the signal off, she said.

Taylor is one of the worlds leading experts on the cAMP-dependent protein kinase, an enzyme that serves as a prototype for the entire protein kinase family. This family of enzymes has more than 500 members that are critical for regulation in all multi-cellular organisms, such as humans.

Taylors work in 1991 (reported in the July 26, 1991 issue of the journal Science) revealed the first-ever molecular structure of the catalytic subunit of a protein kinase, one involved in the action of adrenalin within cells. Understanding its structure was a sort of Rosetta stone for learning the structure of all protein kinases, because they all share certain fundamental characteristics.


'/>"/>

Contact: Debra Kain
ddkain@ucsd.edu
619-543-6163
University of California - San Diego
Source:Eurekalert

Related medicine news :

1. Rural Canadians travel far for specialists: study
2. A new study surpasses Gene Therapy Hurdle
3. Tomato Sauce reduces Cancer Risk- Study
4. A question on study of Adult Stem Cell
5. Study on obesity and heart failure
6. National Lung Study in the process
7. Marijuana gateway theory strengthened by study of twins
8. Old theory of adaptation confirmed by new study
9. Study casts doubt on keyboard ills
10. Gene study links endometriosis, infertility
11. Study reveals how stress can make you sick
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... ... October 13, 2017 , ... Talented host, actor Rob ... sciatica in a new episode of "Success Files," which is an award-winning educational ... and investigates each subject in-depth with passion and integrity. , Sciatica occurs when ...
(Date:10/13/2017)... ... ... “America On The Brink”: the Christian history of the United States and ... of published author, William Nowers. Captain Nowers and his wife, Millie, have six ... years in the Navy. Following his career as a naval aviator and carrier ...
(Date:10/12/2017)... ... , ... Planet Fitness, one of the largest and fastest growing franchisors and ... flagship location in Covington, LA at 401 N. U.S. Highway 190, in January of ... Depot in the Holiday Square shopping center. Its location allows it to serve both ...
(Date:10/12/2017)... ... ... The American College of Medical Informatics (ACMI) will present the 2017 Morris ... of AMIA’s Annual Symposium in Washington, D.C. AMIA’s Annual Symposium is taking ... in the field of medical informatics, this prestigious award is presented to an individual ...
(Date:10/12/2017)... ... October 12, 2017 , ... Dr. Parsa Mohebi, the ... medical article to the newly revamped Cosmetic Town journal section, featuring ... transplant procedure known as Follicular Unit Extraction (FUE). , Dr. Mohebi ...
Breaking Medicine News(10 mins):
(Date:10/2/2017)... -- The Rebound mobile app is poised to become a ... of prescription drug addiction. The app empowers users to develop ... down their dosage in a safe, controlled manner while maximizing ... first 100,000 people to sign up will enjoy 3 months ... ...
(Date:9/27/2017)... their devotion to personalized service, SMP Pharmacy Solutions announces their ... South Florida Business Journal,s 50 Fastest-Growing Companies, and listed for ... specialty pharmacy has found its niche.  To that end, the ... by SFBJ as the 2017 Power Leader in Health Care. ... award in October, Bardisa said of the three achievements, "It,s ...
(Date:9/25/2017)... , Sept. 25, 2017  EpiVax, Inc., a ... design, and immune-engineering today announced the launch of ... development of personalized therapeutic cancer vaccines. EpiVax has ... exclusive access to enabling technologies to the new ... will lead EpiVax Oncology as Chief Executive Officer. ...
Breaking Medicine Technology: