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UCLA researchers create 'fly paper' to capture circulating cancer cells
Date:11/18/2009

the medium, compared with only 4 to 14 percent for the flat device.

"The nanopillar chip captured more than 10 times the amount of cells captured by the currently used flat structure," said lead study author Dr. Shutao Wang, a postdoctoral researcher at both the Crump Institute for Molecular Imaging at the David Geffen School of Medicine at UCLA and the California NanoSystems Institute at UCLA.

Wang noted that the nano-size scale and the unique surface topography of the UCLA nanopillar chip may help it interact with nano-size components on cellular surfaces in the blood, enhancing capture efficiency.

The time required for CTC detection using CellSearch, a technology currently approved by the U.S. Food and Drug Administration, is upwards of three to four hours, according to study author Dr. Hao Wang, a postdoctoral researcher at the Crump Institute and the California NanoSystems Institute at UCLA. The UCLA study found an optimal detection time of only two hours using nanopillar chips.

The nanopillar chip uses a common chamber slide, which fits into standard laboratory cell incubators. After the chip has been incubated and immunofluorescence-stained, an automated fluorescence microscope is used to identify and count the CTCs. The very simple device setting on the chamber slide allows multiple CTC detections to occur at the same time.

"We hope that this platform can provide a convenient and cost-efficient alternative to CTC sorting by using mostly standard lab equipment," said senior study author Dr. Hsian-Rong Tseng, associate professor of molecular and medical pharmacology at the Crump Institute and the California NanoSystems Institute.

The next step is more clinical research and possible studies with "break-away" cancer cells in patients' blood, as well as in other body fluids, such as urine and abdominal fluids, according to Tseng, who is also a researcher at UCLA's Jonsson Comprehensive
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Contact: Rachel Champeau
rchampeau@mednet.ucla.edu
310-794-2270
University of California - Los Angeles
Source:Eurekalert  

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