Navigation Links
Two-lock box delivers cancer therapy
Date:5/6/2014

Rice University scientists have designed a tunable virus that works like a safe deposit box. It takes two keys to open it and release its therapeutic cargo.

The Rice lab of bioengineer Junghae Suh has developed an adeno-associated virus (AAV) that unlocks only in the presence of two selected proteases, enzymes that cut up other proteins for disposal. Because certain proteases are elevated at tumor sites, the viruses can be designed to target and destroy the cancer cells.

The work appears online this week in the American Chemical Society journal ACS Nano.

AAVs are fairly benign and have become the object of intense study as delivery vehicles for gene therapies. Researchers often try to target AAVs to cellular receptors that may be slightly overexpressed on diseased cells.

The Rice lab takes a different approach. "We were looking for other types of biomarkers beyond cellular receptors present at disease sites," Suh said. "In breast cancer, for example, it's known the tumor cells oversecrete extracellular proteases, but perhaps more important are the infiltrating immune cells that migrate into the tumor microenvironment and start dumping out a whole bunch of proteases as well.

"So that's what we're going after to do targeted delivery. Our basic idea is to create viruses that, in the locked configuration, can't do anything. They're inert," she said. When programmed AAVs encounter the right protease keys at sites of disease, "these viruses unlock, bind to the cells and deliver payloads that will either kill the cells for cancer therapy or deliver genes that can fix them for other disease applications."

Suh's lab genetically inserts peptides into the self-assembling AAVs to lock the capsids, the hard shells that protect genes contained within. The target proteases recognize the peptides "and chew off the locks," effectively unlocking the virus and allowing it to bind to the diseased cells.

"If we were just looking for one protease, it might be at the cancer site, but it could also be somewhere else in your body where you have inflammation. This could lead to undesirable side effects," she said. "By requiring two different proteases let's say protease A and protease B to open the locked virus, we may achieve higher delivery specificity since the chance of having both proteases elevated at a site becomes smaller."

In the future, molecular-imaging approaches will be used to detect both the identity and concentration of elevated proteases. "With that information, we would be able to pick a virus device from our panel of engineered variants that has the right properties to target that disease site. That's where we want to go," she said.

Suh said elevated proteases are found around many diseased tissues. She suggested these protease-activatable viruses may be useful for the treatment of not only cancers but also neurological diseases, such as stroke, Parkinson's and Alzheimer's diseases, and heart diseases, including myocardial infarction and congestive heart failure.

The ultimate vision of this technology is to design viruses that can carry out a combination of steps for targeting. "To increase the specificity of virus unlocking, you can imagine creating viruses that require many more keys to open," she said. "For example, you may need both proteases A and B as well as a cellular receptor to unlock the virus. The work reported here is a good first step toward this goal."


'/>"/>

Contact: David Ruth
david@rice.edu
713-348-6327
Rice University
Source:Eurekalert  

Related medicine news :

1. A Revolutionary Dietary Paradigm: New Cookbook Features A SOS-free (Salt, Oil, Sugar ) Diet That Delivers the Optimal Food Plan for Renewing Health
2. Adena Delivers Instant Health Information to Mobile Devices
3. Under-twisted DNA origami delivers cancer drugs to tumors
4. Injectable sponge delivers drugs, cells, and structure
5. John DeLuca, Ph.D., of Kessler Foundation delivers keynote at IPMSC meeting in Milan
6. For stroke patients, mechanical clot removal delivers no advantage over standard care
7. ForeCYTE Delivers Blow in the War Against Breast Cancer
8. 'LKLiveMapping' Improves Laboratory Operating Efficiency, Delivers a High First-Submission Approval of Claims, and Improves Collection
9. Young CEO Delivers Keynote Address to Inspire Collegiate Entrepreneurs at Regional Conference
10. MileageAds.com Ad Network Now Delivers Traffic To Immigration Consultants Business Websites
11. Revenue360® Delivers True Healthcare Pricing Transparency and Significant ROI via its Hospital Revenue Management Solution Suite
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Two-lock box delivers cancer therapy
(Date:9/25/2017)... ... 25, 2017 , ... “Grandpa's Visit”: a tender and relatable tale of ... others. “Grandpa's Visit” is the creation of published author, Beverly Brumfield, gifted writer and ... the character in her book. She shares, “Mr. Rice is struggling to keep up ...
(Date:9/25/2017)... ... , ... The trend-spotters, tastemakers, and healthy lifestyle product experts at the ... drinks, with the new NEXTY Gold Award at the Natural Products Expo East trade ... which is reserved for just those companies that hit high marks on the criteria ...
(Date:9/25/2017)... ... September 25, 2017 , ... Emerald Ash Borers kill Ash trees in as little ... the Philadelphia region. A video by Rob Nagy, ISA Certified Arborist at Giroud Tree ... While several are dead or showing signs of decline from Emerald Ash Borers, two Ash ...
(Date:9/24/2017)... PHOENIX (PRWEB) , ... September 25, 2017 , ... Andrea Purcell, NMD, had an epiphany ... from her nose onto her dinner plate. She thought pregnancy was so hard. She wondered ... concerned about her baby than her. In that moment, she decided to write what she ...
(Date:9/24/2017)... ... September 24, 2017 , ... The Tuscany Resort on Grace Bay in the ... premier boutique resorts, is planning on re-opening the resort on October 1st. ... and more recently Hurricane Maria, and the staff and landscaping crews were fast in ...
Breaking Medicine News(10 mins):
(Date:9/1/2017)... -- Explorers Like Us ( https://explorerslikeus.com/ ) is embarking on "An ... deliver these experiences as part of Life Environments™, a new ... better. ... nothing beats a walk, jog or simply playing in a ... the next best thing when getting there isn’t possible. Life ...
(Date:8/31/2017)... NEW YORK , Aug. 31, 2017 ... the industry,s must-read guide to the latest innovations happening ... the first industry publication to focus on providing a ... advancements. Ultimately, the issue covers the most innovative companies, ... year. "Everyone in ...
(Date:8/29/2017)... 29, 2017 In a move that promises ... compliance for veterinary practices of all sizes, Cubex LLC ... that makes TITAN,s expertise in physical security, drug diversion ... clients nationally. ... controlled substances is at risk today," said TITAN founder ...
Breaking Medicine Technology: