Navigation Links
Two-lock box delivers cancer therapy
Date:5/6/2014

Rice University scientists have designed a tunable virus that works like a safe deposit box. It takes two keys to open it and release its therapeutic cargo.

The Rice lab of bioengineer Junghae Suh has developed an adeno-associated virus (AAV) that unlocks only in the presence of two selected proteases, enzymes that cut up other proteins for disposal. Because certain proteases are elevated at tumor sites, the viruses can be designed to target and destroy the cancer cells.

The work appears online this week in the American Chemical Society journal ACS Nano.

AAVs are fairly benign and have become the object of intense study as delivery vehicles for gene therapies. Researchers often try to target AAVs to cellular receptors that may be slightly overexpressed on diseased cells.

The Rice lab takes a different approach. "We were looking for other types of biomarkers beyond cellular receptors present at disease sites," Suh said. "In breast cancer, for example, it's known the tumor cells oversecrete extracellular proteases, but perhaps more important are the infiltrating immune cells that migrate into the tumor microenvironment and start dumping out a whole bunch of proteases as well.

"So that's what we're going after to do targeted delivery. Our basic idea is to create viruses that, in the locked configuration, can't do anything. They're inert," she said. When programmed AAVs encounter the right protease keys at sites of disease, "these viruses unlock, bind to the cells and deliver payloads that will either kill the cells for cancer therapy or deliver genes that can fix them for other disease applications."

Suh's lab genetically inserts peptides into the self-assembling AAVs to lock the capsids, the hard shells that protect genes contained within. The target proteases recognize the peptides "and chew off the locks," effectively unlocking the virus and allowing it to bind to the diseased cells.

"If we were just looking for one protease, it might be at the cancer site, but it could also be somewhere else in your body where you have inflammation. This could lead to undesirable side effects," she said. "By requiring two different proteases let's say protease A and protease B to open the locked virus, we may achieve higher delivery specificity since the chance of having both proteases elevated at a site becomes smaller."

In the future, molecular-imaging approaches will be used to detect both the identity and concentration of elevated proteases. "With that information, we would be able to pick a virus device from our panel of engineered variants that has the right properties to target that disease site. That's where we want to go," she said.

Suh said elevated proteases are found around many diseased tissues. She suggested these protease-activatable viruses may be useful for the treatment of not only cancers but also neurological diseases, such as stroke, Parkinson's and Alzheimer's diseases, and heart diseases, including myocardial infarction and congestive heart failure.

The ultimate vision of this technology is to design viruses that can carry out a combination of steps for targeting. "To increase the specificity of virus unlocking, you can imagine creating viruses that require many more keys to open," she said. "For example, you may need both proteases A and B as well as a cellular receptor to unlock the virus. The work reported here is a good first step toward this goal."


'/>"/>

Contact: David Ruth
david@rice.edu
713-348-6327
Rice University
Source:Eurekalert  

Related medicine news :

1. A Revolutionary Dietary Paradigm: New Cookbook Features A SOS-free (Salt, Oil, Sugar ) Diet That Delivers the Optimal Food Plan for Renewing Health
2. Adena Delivers Instant Health Information to Mobile Devices
3. Under-twisted DNA origami delivers cancer drugs to tumors
4. Injectable sponge delivers drugs, cells, and structure
5. John DeLuca, Ph.D., of Kessler Foundation delivers keynote at IPMSC meeting in Milan
6. For stroke patients, mechanical clot removal delivers no advantage over standard care
7. ForeCYTE Delivers Blow in the War Against Breast Cancer
8. 'LKLiveMapping' Improves Laboratory Operating Efficiency, Delivers a High First-Submission Approval of Claims, and Improves Collection
9. Young CEO Delivers Keynote Address to Inspire Collegiate Entrepreneurs at Regional Conference
10. MileageAds.com Ad Network Now Delivers Traffic To Immigration Consultants Business Websites
11. Revenue360® Delivers True Healthcare Pricing Transparency and Significant ROI via its Hospital Revenue Management Solution Suite
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Two-lock box delivers cancer therapy
(Date:4/21/2017)... ... April 21, 2017 , ... ... has partnered with O2X , an active lifestyle company that provides Human ... the health of firefighters, police offers, first responders, military officers and others in ...
(Date:4/21/2017)... ... April 21, 2017 , ... Airway Management, the medical ... the highly regarded and well renowned Asian distributor, Discovery Sleep. The ... Nasal Pillow Mask, and will stabilize and strengthen Airway’s presence with a dedicated, ...
(Date:4/21/2017)... ... April 21, 2017 , ... ... seeking candidates to serve on its Accreditation and Standards Committees to support ... mission is improving image quality and reducing patient radiation dose,” reports Eliot ...
(Date:4/21/2017)... ... April 21, 2017 , ... Westside Dental Associates ... dental care since 1985. After thirty-two years, Dr. Latner has become one of the ... my numerous clients over the years with all their dental needs,” said Dr. Latner, ...
(Date:4/21/2017)... ... , ... During April 21-23, Super-Sod will join the vendor market of the ... everyone — from the avid gardener to the landscape admirer — during the three-day ... in a grand venue, and we are certainly looking forward to participating in this ...
Breaking Medicine News(10 mins):
(Date:4/19/2017)... NEW YORK , April 19, 2017 ... Surgical drainage devices are tubes used to remove excess ... include, blood, serum, pus, urine, bile or lymph. Surgical ... types of surgery such as orthopedics surgery, cardiovascular surgery, ... device is prophylactic post-surgery to prevent accumulation of fluid ...
(Date:4/19/2017)... The Global Effective Microorganisms (EM) Market ... has covered and analysed the potential of Global Effective Microorganisms ... shares and growth factors. The report identifies and analyses the ... the global market. ... 152 Tables and Figures, 6 Major Company Profiles, spread across ...
(Date:4/19/2017)... 19, 2017  New research provides evidence that an old ... to a study released today that will be presented at ... in Boston , April 22 to 28, ... of Parkinson,s disease, the oral drug levodopa has long been ... But as the disease progresses, the effects of the medication ...
Breaking Medicine Technology: