Navigation Links
Transplanted liver cells function in older animals but do not proliferate as much as in younger ones
Date:4/19/2009

When things go right, transplanted healthy liver cells transplanted by infusion or injection will find their way to the liver, integrate into the damaged tissue, start proliferating, and take over the liver's work of helping with digestion and removing waste products and worn-out cells from the blood. Hepatocyte transplantation has been successful in a number of animal models, raising hopes that use of cells could overcome the shortage of donor livers and the problems of surgery, but the procedure has not been as successful in humans. Was the problem related to the age of the donor?

Now scientists at the Martin Luther University Hale-Wittenberg, Germany, believe they know the answer. Age of the donor makes no difference but age of the recipient makes a big one. Many humans requiring liver transplantation are older. The German study conducted in rats found that older rats had a repopulation rate of only 2 percent, ten times less than that seen in younger ones. Furthermore, found the scientists, the transplanted cells worked equally well in the old and young animals, as measured by glycogen storage, but the younger animals had significantly higher levels of a growth factor needed for proliferation.

The new study is the first to offer a possible mechanism for the failure of transplanted hepatocytes to proliferate in many human patients and the first to suggest a way to compensate for the problem. Dr. Peggy Stock, a postdoctoral fellow in the laboratory of Dr. Bruno Christ, reported the study on April 19 at Experimental Biology 2009 in New Orleans. The presentation was part of the scientific program of the American Society for Investigative Pathology.

The researchers isolated hepatocytes from both four week old rats (the equivalent age of a human child) and rats more than 35 weeks old (40 to 50 years in humans). These healthy liver cells were then implanted into the livers of young rats (10 weeks, the equivalent of young adulthood) and "senescent" rats (more than 35 weeks).

After six weeks, the amount of hepatocyte proliferation was determined by an assay of CD26 enzyme activity. The recipient animals had been bred to lack the CD26 protein, giving the researchers a way to determine which and how many liver cells were part of the recipient liver and which had the CD26 gene product, indicating they originated from the transplant.

Repopulation of hepatocytes was 20 percent in young rats, whether the cells came from a young or old donor. In older rats, by contrast, repopulation was only 2 percent.

All the transplanted and proliferated cells worked, whether they were transplanted into old or young rats and whether they came from old or young rats. However, IGF1 (insulin like growth factor 1, which regulates growth in the liver and other organs) was significantly higher in the juvenile rats.

The next step, say Dr. Stock and Dr. Christ, is to inject IGF1 in old rats and see if this pretreatment would increase the proliferation levels of hepatocyte cells.


'/>"/>

Contact: Sylvia Wrobel
ebpress@gmail.com
770-722-0155
Federation of American Societies for Experimental Biology
Source:Eurekalert

Related medicine news :

1. Circulating fats kill transplanted pancreas cells, study shows
2. Transplanted Bone Marrow Cells Aid Nerve Regeneration
3. Transplanted Liver Lining Cells May Cure Hemophilia
4. New approaches to protecting transplanted organs make first steps toward patients
5. Transplanted fatty livers associated with worse prognosis for patients with HCV
6. Advanced Age No Bar to Liver Transplant
7. Researchers Find Better Way to Deliver Blood Thinner
8. Federal research plan to determine nanotech risks fails to deliver
9. Liver cancer marker could yield blood test for early detection
10. Blood Marker Might Help Spot Early Liver Cancer
11. Natural Protein Could Help Spot, Treat Liver Cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/26/2017)... ... May 26, 2017 , ... Silver Birch of Hammond, ... is located on more than four acres of land at 5620 Sohl Avenue in ... The 103,000 square-foot building includes 125 studio and one-bedroom apartments. Each of the private ...
(Date:5/26/2017)... (PRWEB) , ... May 26, 2017 , ... “THE FLINTHILLS FAMILY-Our Journey to the Cross”: ... FAMILY-Our Journey to the Cross” is the creation of published authors, Bob and Margaret Massey. ... used to say, he is "panther quick and leather tough." His love for others ...
(Date:5/26/2017)... ... 2017 , ... Water damage to the flooring of several classrooms at The ... officials with a number of critical issues to address before students could return to ... with little or no disruption to class schedules. Second, the project had to comply ...
(Date:5/24/2017)... ... , ... Altec Products, Inc., a leader in enterprise document management ... conference in San Diego, CA. , At nVerge 2017, Altec will be highlighting ... enhance their Sage ERP solutions by providing improved visibility and control to the entire ...
(Date:5/24/2017)... ... May 24, 2017 , ... Patients who want to receive cosmetic dentistry procedures ... Dr. Joseph Bedich for a consultation, with or without a referral. Dr. Bedich enjoys ... functionality. , Dr. Bedich offers a variety of cosmetic dentistry services at ...
Breaking Medicine News(10 mins):
(Date:5/22/2017)... 2017  As the specialty pharmacy industry and ... make the revolutionary shift from volume-based to value-based ... positive patient outcomes and shaping the future of ... away from clinical trials and toward data that ... drug therapy utilization in precise patient populations. Therigy ...
(Date:5/15/2017)... , May 15, 2017 Enterin Inc., a ... and developing novel compounds to treat Parkinson,s disease (PD), ... study is a Phase 1/2a randomized, controlled, multicenter study involving ... It will enroll 50 patients over a 9-to-12-month period. The ... in 10 patients with PD. Participating sites include ...
(Date:5/10/2017)... May 10, 2017 Global Health Intelligence ... Latin America , published its 2017 ranking of the ... based on extensive data analysis from GHI,s hospitals database ... database for the region. The GHI database covers 86% of ... more than 130 data points for each institution in key ...
Breaking Medicine Technology: