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Tracking down smallest biomarkers
Date:11/27/2012

Microvesicles are smallest cell elements which are present in all body fluids and are different, depending on whether a person is healthy or sick. This could contribute to detecting numerous diseases, such as, e.g., carcinomas, at an early stage, and to treating them more efficiently. The problem is that the diameter of the relevant microvesicles generally lies below 100 nm, which makes them technically detectable, but their exact size and concentration hardly possible to determine. A new device is now to provide the metrological basis for these promising biomarkers. The vacuum-compatible version of the Pilatus hybrid pixel detector for X-rays, which was developed by Dectris in cooperation with the Physikalisch-Technische Bundesanstalt (PTB), now allows also the size of nano-particles which, to date, have been difficult to characterize to be determined using small-angle X-ray scattering at low photon energies. The detector can also be used for other X-ray-based techniques.

What makes this detector unique is the size of its total surface (17 cm 18 cm) as well as the fact that it can be operated in vacuum. Operating the detector in vacuum drastically increases the sensitivity of the measuring facility, since the soft X-rays, which are scattered on the sample, are not absorbed by air molecules on their way towards the detector. This device now allows, for example, experiments for size determination of nanoparticles to be carried out with small-angle X-ray scattering (SAXS) also at the absorption edges of the light elements calcium, sulphur, phosphor or silicon at photon energies below 5 keV with high dynamics and good spatial resolution.

For a few months, the new Pilatus X-ray detector has been used for some of PTB's own research projects. At the synchrotron radiation source BESSY II in Berlin-Adlershof, where PTB has been operating its own laboratory for 15 years, scientists are now using the new detector, for example, to establish the urgently n
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Contact: Michael Krumrey
michael.krumrey@ptb.de
49-030-348-17110
Physikalisch-Technische Bundesanstalt (PTB)
Source:Eurekalert  

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