Navigation Links
Tracing unique cells with mathematics
Date:1/23/2014

This news release is available in German.

Each cell in our body is unique. Even cells of the same tissue type that look identical under the microscope differ slightly from each other. To understand how a heart cell can develop from a stem cell, why one beta-cell produces insulin and the other does not, or why a normal tissue cell suddenly mutates to a cancer cell, scientists have been targeting the activities of ribonucleic acid, RNA.

Proteins are constantly being assembled and disassembled in the cell. RNA molecules read blueprints for proteins from the DNA and initiate their production. In the last few years scientists around the world have developed sequencing methods that are capable of detecting all active RNA molecules within a single cell at a certain time.

At the end of December 2013 the journal Nature Methods declared single-cell sequencing the "Method of the Year." However, analysis of individual cells is extremely complex, and the handling of the cells generates errors and inaccuracies. Smaller differences in gene regulation can be overwhelmed by the statistical "noise."

Easier and more accurate, thanks to statistics

Scientists led by Professor Fabian Theis, Chair of Mathematical modeling of biological systems at the Technische Universitaet Muenchen and director of the Institute of Computational Biology at the Helmholtz Zentrum Muenchen, have now found a way to considerably improve single-cell analysis by applying methods of mathematical statistics.

Instead of just one cell, they took 16-80 samples with ten cells each. "A sample of ten cells is much easier to handle," says Professor Theis. "With ten times the amount of cell material, the influences of ambient conditions can be markedly suppressed." However, cells with different properties are then distributed randomly on the samples. Therefore Theis's collaborator Christiane Fuchs developed statistical methods to still identify the single-cell properties in the mixture of signals.

Combining model and experiment

On the basis of known biological data, Theis and Fuchs modeled the distribution for the case of genes that exhibit two well-defined regulatory states. Together with biologists Kevin Janes and Sameer Bajikar at the University of Virginia in Charlottesville (USA), they were able to prove experimentally that with the help of statistical methods samples containing ten cells deliver results of higher accuracy than can be achieved through analysis of the same number of single cell samples.

In many cases, several gene actions are triggered by the same factor. Even in such cases, the statistical method can be applied successfully. Fluorescent markers indicate the gene activities. The result is a mosaic, which again can be checked to spot whether different cells respond differently to the factor.

The method is so sensitive that it even shows one deviation in 40 otherwise identical cells. The fact that this difference actually is an effect and not a random outlier could be proven experimentally.


'/>"/>
Contact: Andreas Battenberg
battenberg@zv.tum.de
49-892-891-0510
Technische Universitaet Muenchen
Source:Eurekalert  

Related medicine news :

1. Tracing the Paralympic movements freak show roots
2. Unique approach needed to accurately assess health of young adult cancer survivors
3. Unique physiology key to diagnosing and treating diabetes in Asian populations
4. FirstVitals Health and Wellness, Inc. awarded $4 Million CMS Innovation Grant for Unique Telehealth Diabetes Progam Focused on Glycemic Control and Prevention of Diabetic Foot Complications
5. Unique program bringing electronic medical record data to ambulances lauded
6. LA BioMed offers unique educational and research opportunity to local high school seniors
7. Heat-shock factor reveals its unique role in supporting highly malignant cancers
8. Brain Scans of Hoarders Show Unique Abnormalities
9. Moffitt Cancer Center melanoma expert reviews unique adverse events with newly approved drug
10. A CNIO team creates a unique mouse model for the study of aplastic anaemia
11. Kings to develop unique sunscreen with Aethic
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Tracing unique cells with mathematics
(Date:4/28/2016)... ... April 28, 2016 , ... Accreditation Commission for ... Pharmacy Association (COPA) to develop a comprehensive Specialty Pharmacy Accreditation with Distinction in ... accreditation distinction. ACHC provides a wide range of pharmacy accreditation services that assess ...
(Date:4/28/2016)... ... April 28, 2016 , ... The Aging in Motion Coalition ... ICD-10-CM code for sarcopenia, giving it recognition for separate reporting and data collection. The ... 2016. , Sarcopenia is defined as a combination of low muscle mass and weakness ...
(Date:4/28/2016)... ... April 28, 2016 , ... The USDA recently released its updated Dietary Guidelines ... science. While there is a lot of information available in the report, older men ... 2016 issue of Harvard Men's Health Watch . , These include: , ...
(Date:4/28/2016)... ... April 28, 2016 , ... Dr. Farhan Qureshi has ... been named a 2015 Top Rated Alexandria Dentist from Find Local ... has received across many credible sources. Find Local Doctors is an online directory that ...
(Date:4/28/2016)... ... April 28, 2016 , ... Vigamox and other pink eye medications ... contagious infection of the mucus membrane of the eye. This membrane, the conjunctiva, ... eye, also called conjunctivitis, can be caused by a virus, bacteria, extreme dryness, chemicals ...
Breaking Medicine News(10 mins):
(Date:4/28/2016)... , April 28, 2016 Research ... "Global Plastic Surgery Products Market 2016-2020" report to ... ) , The global plastic surgery products ... 9.47% during the period 2016-2020. , ,The growing adoption ... to the growth of the market. Lasers are used ...
(Date:4/28/2016)... , Net Sales of $1.90 billion represent ... prior year period, and an increase of 1.2% on an ... for the first quarter were $0.52 reported, a decrease of ... increase of 29.9% over the prior year period , ... for 2016 Zimmer Biomet Holdings, Inc. (NYSE and ...
(Date:4/28/2016)... , April 28, 2016 ... development of innovative peptide and gene-based immunotherapeutics and vaccines for ... will be presenting at the 3rd Annual Growth Capital ... 5 th , 2016 at Caesars Palace in Las Vegas, Nevada.  ... am on Wednesday, May 4 th by Dr. ...
Breaking Medicine Technology: