Navigation Links
Toothpick: New molecular tag IDs bone and tooth minerals
Date:7/10/2008

Enlisting an army of plant viruses to their cause, materials researchers at the National Institute of Standards and Technology (NIST) have identified a small biomolecule that binds specifically to one of the key crystal structures of the bodythe calcium compound that is the basic building block of teeth and bone. With refinements, the researchers say, the new molecule can be a highly discriminating probe for a wide range of diagnostic and therapeutic applications related to bones and teeth.

Although they have somewhat different mechanical properties, the major structural component of both teeth and bones is a crystalline compound of calcium phosphate called hydroxyapatite. Subtle variations in the way the crystal forms account for the differences. Identifying and monitoring the formation of this particular crystal is of paramount importance to biomedical researchers working on a variety of problems including the remineralization of teeth to repair decay damage, the integration of prosthetic joints and tissue-engineered bone materials for joint and bone replacement, and cell-based therapies to regrow bone tissue.

To date, however, there is no specific, practical method to spot the formation of hydroxyapatite in living systems or tissue samples. Materials scientists can identify the crystal structure with high reliability by the pattern it makes scattering X rays, but it's a complex procedure, requires fairly pure samples and certainly can't be used on living systems. There are some widely used chemical assaysthe von Kossa assay, for examplebut these also are destructive tests, and more importantly they really test simply for the presence of the elements calcium or phosphorus. They can't distinguish, for example, between deposits of amorphous calcium phosphatea precursorand the hydroxyapatite crystal.

To find a more specific, less destructive probe, the NIST team used a relatively new technique called "phage display" that can rapidly create and screen huge numbers of biomolecules for specific interactions. Phages are a primitive and ubiquitous class of viruses that infect bacteria. Some simple phages can be genetically modified to randomly assemble short sequences of amino acidssmall proteins called peptideson their outer shells as binding sites. An engineered population of phages will synthesize billions of random peptides. If these phages are exposed to the target surfacehydroxyapatite crystal in this caseand then washed off, those left behind are the ones that tend to stick. Cloning the survivors and repeating in several cycles with increasingly stringent conditions eventually isolates a handful of candidate peptides that can be further tested to measure their affinity for the target.

As reported in a recent paper,* the NIST team used the technique to identify a new peptide that relies both on the chemical composition and the crystal structure of hydroxyapatite to bind to the mineral's surface. The peptide's ability to "recognize" the specific structure of hydroxapatite, say the researchers, could be exploited as a nondestructive tag to monitor the progress of bone and tooth mineralization for diagnostic and therapeutic applications.


'/>"/>

Contact: Michael Baum
michael.baum@nist.gov
301-975-2763
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Related medicine news :

1. Molecular motor works by detecting minute changes in force
2. Alcoholism-associated molecular adaptations in brain neurocognitive circuits
3. Schizophrenia linked to dysfunction in molecular brain pathway activated by marijuana
4. New research sheds light on the molecular basis of crib death
5. Solis Womens Health Brings Molecular Breast Imaging to Dallas-Fort Worth Metroplex
6. New Molecular Trigger Described for Hypertension, Diabetes
7. New molecular insight into amboebic dysentery
8. Molecular insight begins Trofex Phase 1 trial for detection of metastatic prostate cancer
9. Molecular insight begins Trofex Ph. 1 trial for detection of metastatic prostate cancer
10. Siemens Showcases Innovative Leadership in Molecular Imaging at SNM 2008
11. Xceed Molecular Licenses Signature from Moffitt Cancer Center and Adds Moffitt to Strategic Collaborator Program
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Toothpick: New molecular tag IDs bone and tooth minerals
(Date:8/18/2017)... ... August 18, 2017 , ... The Golseth Agency, a Texas based insurance management ... spearheading a regional charity campaign organized to provide support to Christina Upchurch and her ... year, Christina and her children returned from out of town to find her husband ...
(Date:8/18/2017)... ... August 18, 2017 , ... Alcovit, a lime-flavored beverage that ... its marketing efforts with its product now available through Jet.com. , After 25 ... The effervescent powdered drink is designed to quickly detox the body thereby avoiding alcohol-induced ...
(Date:8/18/2017)... ... ... “Dangerous Inheritance”: a mystery about saving the family farm. “Dangerous Inheritance” is the ... was raised on a farm. As Diane wrote this story, she used fond ... mantra of ‘neighbors helping neighbors’ have always been a large part of her farming ...
(Date:8/18/2017)... ... August 18, 2017 , ... Rollon Skin ... anti-aging skincare solutions, recently announced the launch of two new skincare products, ... yet effective alternative to expensive plastic surgery or in-patient cosmetic dermatological procedures. , ...
(Date:8/18/2017)... ... August 18, 2017 , ... ... Coatesville Veterans Affairs Medical Center. “Case #10-5411 Veterans Administration” is the creation ... domestic abuse. In June 1990, Carol Mulhem started work at the Coatesville ...
Breaking Medicine News(10 mins):
(Date:7/31/2017)... -- 7D Surgical, developer of ground breaking surgical navigation technologies, ... System to support its strategic sales plan in ... Virginia.  7D Surgical has entered into an exclusive sales representative ... facilities within those markets. ... Spartan Medical Purchases 7D ...
(Date:7/27/2017)... 2017  Zimmer Biomet Holdings, Inc. (NYSE and SIX: ZBH) ... 30, 2017.  The Company reported second quarter net sales ... prior year period, and an increase of 2.1% on ... of contribution from the LDR Holding Corporation acquisition, second ... of 2016, or 0.3% on a constant currency basis. ...
(Date:7/26/2017)... Aesthetics, a leading medical aesthetic clinic in Singapore ... skin conditions from the inside. The natural process of ... skin becomes more transparent due to the thinning of the epidermis. Over ... the sun contributes to aging skin, causing age spots and other ... ...
Breaking Medicine Technology: