Navigation Links
Team solves mystery associated with DNA repair
Date:12/13/2012

ave shown that in bacteria, RecA forms a filament that winds itself around a broken, single strand of DNA. Like a matchmaker trying to find a partner for an unpaired dancer, it scours the corresponding DNA strands for a sequence that will pair up perfectly with the broken strand. Once it finds the sequence, the broken strand steps in and chemically bonds to its new partner, displacing one of the unbroken strands (which eventually pairs with the other broken strand). This elaborate molecular square dance allows the cell to go back to the work of duplicating its genome. Each broken strand now is paired with an unbroken one, and uses the intact strand as a template for replication. (Watch an animation about this process.)

"If a break in DNA occurs, you have to repair it," Ha said. "We wanted to know how RecA helps the DNA find a sequence complementary to it in the sea of genomic DNA, and how it does it so quickly."

To answer this question, the researchers made use of fluorescence resonance energy transfer (FRET) to observe in real time the interaction of the RecA protein and the DNA. FRET uses fluorescent molecules whose signals vary in intensity depending on their proximity to one another. By labeling a single DNA strand bound by RecA and putting a different fluorescent label on a stretch of double-stranded DNA, the researchers could see how the molecules interacted with one another.

The team determined that RecA that is bound to a broken, single-stranded DNA molecule actually slides back and forth along the double-stranded DNA molecule searching for a match.

"We discovered that this RecA filament can slide on double-stranded DNA for a span of sequences covering about 200 base pairs of DNA," Ha said. "This is how one strand of DNA can be exchanged with another from a different DNA duplex. That's the process called 're
'/>"/>

Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3

Related medicine news :

1. Solving a biological mystery
2. Cell death mystery yields new suspect for cancer drug development
3. Danish scientists solve old blood mystery
4. Solving the Medical Mystery of Cold Feet
5. Solving the mystery of how cigarette smoking weakens bones
6. 50-year cholera mystery solved
7. Mystery of the missing breast cancer genes
8. Scientists solving the mystery of human consciousness
9. Cleveland Clinic researcher identifies 2 new genetic mutations associated with Cowden syndrome
10. Study suggests vision insurance associated with eye-care visits, better reported vision
11. Pitt research sheds new light on virus associated with developmental delays and deafness
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Team solves mystery associated with DNA repair
(Date:7/11/2014)... July 11, 2014 Presently, disposable medical ... healthcare equipment and supplies segment. During the past two ... steady growth (on an average 12.5% per year), primarily ... settings. Currently, the US is the largest market for ... share of 40%. The second position is held by ...
(Date:7/11/2014)... -- Researchers from The Miriam Hospital have found ... have significantly higher rates of smoking than those ... were less likely to attempt quitting than those ... not be sufficient for this population. The study ... of print in the American Journal of ...
(Date:7/11/2014)... PULLMAN, Wash.The largest study of its kind has ... suite of advantages over their conventional counterparts, including ... , The study looked at an unprecedented 343 ... of organic and conventional plant-based foods, including fruits, ... meta-analysis techniques to quantify differences between organic and ...
(Date:7/11/2014)... FRIDAY, July 11, 2014 (HealthDay News) -- Delaying surgery to ... main ligament in the middle of the knee -- could ... They analyzed the medical records of 130 patients, aged ... patients, 62 had surgery less than six weeks after their ... the others had surgery more than three months after their ...
(Date:7/11/2014)... 11, 2014 (HealthDay News) -- Prostate cancer patients are ... less effective treatment and die sooner if they also ... data from more than 41,200 American men who were ... They followed them through 2009. Nearly 1,900 of the ... years before their prostate cancer was discovered. "Men ...
Breaking Medicine News(10 mins):Health News:Global Disposable Medical Supplies Market to See Robust Growth Through Next Decade, According to New TriMark Report Available at MarketPublishers.com 2Health News:Global Disposable Medical Supplies Market to See Robust Growth Through Next Decade, According to New TriMark Report Available at MarketPublishers.com 3Health News:Miriam Hospital study examines smoking prevalence 2Health News:Major study documents nutritional and food safety benefits of organic farming 2Health News:Major study documents nutritional and food safety benefits of organic farming 3Health News:Delaying Kid's Knee Surgery Could Be a Bad Play, Study Finds 2Health News:Depression May Make It Harder to Beat Prostate Cancer 2
... BOSTON--A surprise discovery -- that calorie-burning brown fat can be ... the prospect of new ways to fight obesity and overweight, ... Aug. 21 issue of the journal Nature, the ... "good" form of fat -- so called because it burns ...
... of new ways to treat obesity , , WEDNESDAY, Aug. ... also helps promote development of "good" brown fat that helps ... researchers at the Joslin Diabetes Center in Boston. , They ... lead to new ways to prevent and treat obesity. , ...
... Aug. 20 Digital health technologies designed to,monitor ... in U.S. revenues in,excess of $1.8 billion by ... in service and hardware,revenues, according to Parks Associates, ... medical, activity, and environmental,sensors and fitness measurement devices ...
... Aug. 20 Cord Blood America, Inc.,(OTC Bulletin ... preservation,company ( http://www.cordblood-america.com ) focused on bringing the ... and internationally,today reported that revenues for the first ... million., Revenues were down from the same ...
... fuels rapid growth by partnering with Flexicare Ltd., a ... intubation products]. , ... ... week that it had expanded its product offering of ...
... AggreWell™400 plate is a novel tool for standardizing the generation ... ... Vancouver, British Columbia (PRWEB) August 20, 2008 -- http://www.stemcell.com ... cell separation products and ancillary reagents for stem cell research, ...
Cached Medicine News:Health News:Making 'good' fat from muscle and vice versa 2Health News:Making 'good' fat from muscle and vice versa 3Health News:Bone Growth Protein Also Promotes 'Good' Fat 2Health News:Service and Hardware Revenues Will Contribute $1.8 Billion to the U.S. Eldercare Industry by 2013 2Health News:Cord Blood America Reports Six Month Financial Results 2Health News:ORSupply.com Introduces New Line of Products from Flexicare 2Health News:STEMCELL Technologies Introduces AggreWell™400 for Standardized Embryonic Stem Cell and Induced Pluripotent Stem Cell Research 2
(Date:1/15/2014)... Corporation (the "Company") (NASDAQ: CLSN ) today ... to purchase an aggregate of approximately $15 million of ... offering, led by a dedicated health care fund. ... these investors pursuant to which the Company agreed to ...
(Date:1/15/2014)... 2014 Shire plc (LSE: SHP, NASDAQ: ... Trading (OFT) approval condition to the announced tender offer for all ... As a result of the waiver, the transaction is no ... tender offer on January 24, 2014 following the currently scheduled expiration ...
(Date:1/15/2014)... BreedIT Corp (OTC: BRDT), through its Israeli ... sophisticated agro-breeding solutions for plant breeders and researchers, today ... of directors appointed chemistry and pharmaceutical industry expert Dr. ... the board. From 1975 to 2012, Dr. ...
Breaking Medicine Technology:Celsion Corporation Announces $15 Million At-The-Market Registered Direct Offering 2Celsion Corporation Announces $15 Million At-The-Market Registered Direct Offering 3Shire Waives OFT Condition Relating to the Acquisition of ViroPharma Inc. 2Shire Waives OFT Condition Relating to the Acquisition of ViroPharma Inc. 3BreedIT (OTC: BRDT) Appoints Chemistry and Pharmaceutical Industry Expert Dr. Ben-Zion Weiner to Its Board of Directors 2BreedIT (OTC: BRDT) Appoints Chemistry and Pharmaceutical Industry Expert Dr. Ben-Zion Weiner to Its Board of Directors 3
This is the modular VICTOR2 multilabel counter with the TR-fluorometry, stacker and robot loading options.The addition of a xenon flash lamp brings the time-resolved fluorescence counting mode to th...
Flexible and sensitive fluorescence/luminescence microplate reader for endpoint and kinetic...
The DELFIA Platewash is a general purpose microtitration plate and strip washer especially standardized for use with DELFIA diagnostic kits....
... with a 25-microplate capacity ... and aspiration and dispense ... On-board software for user ... programs. External vacuum pump ...
Medicine Products: