Navigation Links
Swine cells could power artificial liver
Date:2/27/2013

Chronic or acute, liver failure can be deadly. Toxins take over, the skin turns yellow and higher brain function slows.

"There is no effective therapy at the moment to deal with the toxins that build up in your body," said Neil Talbot, a Research Animal Scientist for the USDA Agricultural Research Service. "Their only option now is to transplant a liver."

Talbot thinks a line of special liver cells could change that. In an interview with the American Society of Animal Science, he discussed how a line of pig liver cells called PICM-19 could perform many of the same functions as a human liver.

In 1991, Talbot created PICM-19 from the cells of an 8-day-old pig embryo. The cell line is significant because it is "immortal," meaning the cells can divide an infinite number of times. Many immortal cells lines continue dividing because they are derived from cancer cells; however, PICM-19 cells are derived from epiblast cells, the embryonic stem cells that form in the early stages of embryo development.

This immortal cell line has helped Talbot study how cells differentiate. Cells from the PICM-19 lines naturally differentiate into bile duct cells or hepatocytes. Hepatocytes do the bulk of the work in a liver. Hepatocytes form and secrete bile, store glycogen, control blood glucose, process vitamin D, and metabolize cholesterol and fat.

"The PICM- 19 cells are the cells that really do all the metabolic functions of the liver," said Talbot.

Hepatocytes also "scrub" toxins from the blood. Talbot said PICM-19 cells could do the same thing inside an artificial liver. There have already been several in vitro tests of artificial liver devices, and the ARS scientists are working on ways to grow the PICM-19 cells without needing "feeder cells." Feeder cells are mouse cells that hold PICM-19 cells in place and provide important molecules for PICM-19 cell growth and maintenance.

Artificial livers are still in development, but Talbot pointed out other applications for PICM-19 cells. Talbot and fellow scientists have used PICM-19 to study malaria, toxoplasmosis and hepatitis viruses. Researchers could also use the cells to study certain cancers of the liver or investigate the changes in the bile duct associated with cystic fibrosis.

Talbot recommends future studies on how PICM-19 cells respond to selective pressures. He said scientists could select for more efficient liver cells by exposing PICM-19 cells to toxins in culture.

"A lot of cells would die, but the survivors would really be tough," Talbot said.

Those tougher cells could make artificial liver devices more effective. Scientists could also use genetic modification to prompt PICM-19 cells to behave like other cells in the body.

"Maybe we want to enable it to make insulin," Talbot said. "It will be like a pancreas."

With PICM-19 cells filling in for livers or other organs, the transplant list could get a lot shorter.

Tom Caperna, an ARS Research Biologist and collaborator with Talbot, presented their work on PICM-19 during the Growth and Development Symposium at the 2012 Joint Annual Meeting. The full symposium summary is titled "Growth and Development Symposium: Development, characterization, and use of a porcine epiblast-derived stem cell line: ARS-PICM-19." It can be read in full at journalofanimalscience.org.


'/>"/>

Contact: Madeline McCurry-Schmidt
madelinems@asas.org
217-689-2435
American Society of Animal Science
Source:Eurekalert

Related medicine news :

1. H1N1 Swine Flu Vaccine Tied to Sleep Disorder in British Children
2. Healthy-Looking Pigs at State Fair Found to Have Swine Flu
3. Sharp Spike Seen in Swine Flu Cases: CDC
4. CDC Preparing Vaccine for New Swine Flu
5. New study shows why swine flu virus develops drug resistance
6. Now hear this: Stanford researchers identify forerunners of inner-ear cells that enable hearing
7. Study reveals stem cells in a human parasite
8. OHSU scientists first to grow liver stem cells in culture, demonstrate therapeutic benefit
9. Distinct niches in bone marrow nurture blood stem cells
10. Reprogramming cells to fight diabetes
11. First signals from brain nerve cells with ultrathin nanowires
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/19/2017)... ... August 18, 2017 , ... Paragon and ... to help the cannabis industry move towards greater legitimacy. By incorporating IOTA’s unique ... technology with lower fees. Paragon will build a fully verifiable database to track ...
(Date:8/19/2017)... ... August 19, 2017 , ... Praeclarus Press ... mothers. These illustrations show the diversity of the breastfeeding mothers, using bright ... range of sizes. These illustrations are also available on tote bags, notepads, smartphone ...
(Date:8/19/2017)... , ... August 19, 2017 , ... “Congratulations! It's A ... of children to both their parents and God. “Congratulations! It's A Boy! God’s Gift: ... Anthony, a couple from Long Island, New York. , Published by Christian Faith Publishing, ...
(Date:8/18/2017)... New York (PRWEB) , ... ... ... Courier, LLC, d/b/a Quick International, LLC (“Quick”), a highly specialized asset-light logistics ... it has entered into a definitive agreement to purchase Unitrans International Corporation, ...
(Date:8/18/2017)... ... August 18, 2017 , ... ... handheld devices. Through an educational webinar, they will present the line of epMotion ... to learn how easy you can automate everyday pipetting tasks. , Ideal for ...
Breaking Medicine News(10 mins):
(Date:8/15/2017)... N.C. , Aug. 15, 2017  Axium Pharmaceuticals Inc., the creator ... rumored to be in the beginning stages of an IPO. ... on epilepsy medications with the average cost of a prescription epilepsy drug ... ... Another staggering figure is the fact ...
(Date:8/11/2017)... , Aug. 11, 2017 DarioHealth Corp. ... mobile health and big data solutions, today announced that ... Monday, August 14 and host a conference call at ... 2017 operating and financial results and its strategy and ... be hosted by Erez Raphael , Chief Executive ...
(Date:8/7/2017)... GAITHERSBURG, Md. , Aug. 7, 2017 /PRNewswire/ ... for healthcare member acquisition, retention, and engagement, announced ... Director of Strategy and Product Development, effective as ... strategic consulting and technology implementation strategy for our ... years of experience in consulting and business analytics ...
Breaking Medicine Technology: