Navigation Links
Study suggests enzyme crucial to DNA replication may provide potent anti-cancer drug target
Date:4/14/2011

w complementary strand.

That task is fairly straightforward on what is known as the "leading" of the two strands. The replication fork moves along from the so-called 3' (three prime) end to the 5' (five prime) end, and DNA polymerase synthesizes a 5' to 3' complementary strand.

But because the two strands are anti-parallel, meaning they are oriented in opposite directions, the work of DNA polymerase, which can only work in the 5' to 3' direction, is more difficult on the so-called lagging strand. This strand needs to be replicated in pieces, which are known as Okazaki fragments, located near the replication fork. These fragments include a "primer," a strand of RNA that serves as a starting point for DNA synthesis.

This is where FEN1 comes init removes that RNA primer on the 5' flap, which occurs every 100 base pairs or so on the lagging strand, said Tainer. It's an enormous job that has to be done rapidly and accurately in order to glue the ends of replicated DNA on the lagging strand together to eventually provide an intact chromosome. "To replicate one DNA double helix in one cell you have to cut off a 5' flap so that you don't have one base pair too many or one base pair too few, and you have to do this accurately with 50 million Okazaki primers in each cell cycle," Tainer said. "It has always been a mystery as to how FEN1 can precisely cut this flap so efficiently and so rapidly. It's an amazing, efficient molecular machine for precisely cutting DNA."

To determine what FEN1 looked like in action, Arvai led the difficult but ultimately successful effort to grow crystals of the human FEN1 protein bound to DNA. The team then used X-ray crystallography to determine the atomic structure of the complex. Using Lawrence Berkeley National Laboratory's Advanced Light Source beamline, called SIBYLS, the scientists solved three different crystal structures.

The end result was a highly detailed and accurate model showing the stru
'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3 4

Related medicine news :

1. Despite Treatment, Employees with Depression Generate Higher Absentee Costs, According to Thomson Reuters Study
2. American Council on Exercise (ACE) Study Reveals Kettlebells Provide Powerful Workout in Short Amount of Time
3. TV drama can be more persuasive than news program, study finds
4. Study carried out into biological risks of eating reptiles
5. Neuroimaging study may pave way for effective Alzheimers treatments
6. Study finds racial gaps continue in heart disease awareness
7. Luth Researchs IndicatorEDG(TM) Study Finds Americans Hopes of Achieving Their Dreams Are Fading
8. First blinded study of venous insufficiency prevalence in MS shows promising results
9. Soothing infants with food focus of childhood obesity study
10. People with anxiety disorder less able to regulate response to negative emotions, study shows
11. American Heart Association Rapid Access Journal Report: Study Finds Racial Gaps Continue in Heart Disease Awareness, Low Knowledge of Heart Attack Warning Signs Among Women
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/29/2015)... ... 30, 2015 , ... On September 30, 2015, the Career and Transfer Center ... accredited Maricopa Community Colleges , will be hosting a Job Expo to provide ... and opportunities. , The Expo has expanded to much more than just a typical ...
(Date:8/29/2015)... ... 29, 2015 , ... On Saturday, September 19, 2015, Diane Blackburn-Zambetti, the Director ... Foundation) will present an educational program titled “Asbestos and Your Health.” , The two ... (doors open at 6:45pm) at Calvary United Methodist Church, 16 E Park Ave., Ambler, ...
(Date:8/29/2015)... (PRWEB) , ... August 29, 2015 , ... ... to insufficient scrotal support and protection against dribbled urine and sweat. "In order ... Manhasset Hills, N.Y. , The EASY ACCESS DRYNESS ENHANCED MEN'S UNDERGARMENT provides optimal ...
(Date:8/29/2015)... CA (PRWEB) , ... August 29, 2015 , ... Dr. ... a mission. His mission is to increase acceptance of the chiropractic field in ... medications that further debilitate them. Although Chiropractic was made available to the VA ...
(Date:8/29/2015)... NY (PRWEB) , ... August 29, 2015 , ... Next ... end of summer (which astronomically, officially ends on September 22nd this year). For IT ... cyber security hangovers linger on which could prove far more costly than a simple ...
Breaking Medicine News(10 mins):Health News:Estrella Mountain Community College Expo Offers ‘One-Stop’ for Employers and Students Seeking Internships, Jobs, Careers and Service Learning Opportunities 2Health News:Mesothelioma Applied Research Foundation to Hold Educational Class About Asbestos and Mesothelioma Prevention 2Health News:Modified Men’s Underwear Promote More Comfort and Hygiene - Designed by InventHelp Client (LGI-1968) 2Health News:The Patriot Project brings Chiropractic Care to the Armed Forces and their Families - Rapid Release Technology Joins the Effort to Recruit more Doctors to Pledge 2Health News:Business Cyber Security – Come Out of the Summer with a Clean Slate 2Health News:Business Cyber Security – Come Out of the Summer with a Clean Slate 3Health News:Business Cyber Security – Come Out of the Summer with a Clean Slate 4
... ... FEHBP oppose a new effort by Congress to reduce pharmacy choices, arbitrarily ... in the program. FEHBP enrollees feel that OPM does a great ... than help the program by forcing OPM to change FEHBP’s pharmacy benefits. ...
... with operable disease, review finds , WEDNESDAY, March 24 ... operable non-small cell lung cancer, say researchers who reviewed ... studies involving almost 8,500 patients (and more than 3,300 ... percent for patients who had chemotherapy and surgery, and ...
... Obama signed into law U.S. health care reform legislation, ... their families, and businesses. The national health ... in Massachusetts requiring all adults in the Commonwealth to ... new article from The Milbank Quarterly , by ...
... University in Silicon Valley will host a one-day workshop ... focus on the role of high-tech communication and other ... plan to identify mutually beneficial research agendas and exchange ... campus," said Martin Griss, director of Carnegie Mellon,s Silicon ...
... damage to a neural center for emotions don,t get ... 24 (HealthDay News) -- Your ability to judge wrongdoing ... based in a part of the brain that regulates ... Institute of Technology report that people who suffer from ...
... come loaded with vitamin E, to help more medicine get ... -- A team of researchers has created special contact lenses ... a design that could essentially lengthen the amount of time ... reduce the significant waste of medication that happens with traditional ...
Cached Medicine News:Health News:PCMA: Federal Enrollees Don't Want Congress to Reduce Pharmacy Choices, Impose Price Controls in FEHBP 2Health News:Chemo May Boost Survival After Lung Cancer Surgery 2Health News:Massachusetts health care reform reviewed as a model for national plan 2Health News:Brain's 'Moral Outrage' Center Pinpointed 2Health News:New Contact Lenses Could Improve Glaucoma Treatment 2Health News:New Contact Lenses Could Improve Glaucoma Treatment 3
(Date:8/28/2015)... -- Perrigo Company plc ("Perrigo") (NYSE: PRGO ; ... MYL ) shareholder vote regarding its planned unsolicited ... Mylan,s offer to Perrigo shareholders have always been, and ... reflection of the value available to Perrigo shareholders, and ... has allowed its shareholders to consider," said Joseph ...
(Date:8/28/2015)... , Joint research by Prof. Kim ... , Published in ,Stem Cells Translational Medicine, (IF ... multiple IV administration of Adipose-derived MSC , Increase ... , Commercialization within 5 years expected ... adult mesenchymal stem cells (MSCs) may extend healthy life ...
(Date:8/28/2015)... , August 28, 2015 The ... reach USD 49.12 billion by 2020, growing at a ... a new report by Grand View Research, Inc. Monitoring ... of 49.7% from 2014 to 2020.      ... population along with rising incidences of chronic diseases such ...
Breaking Medicine Technology:Perrigo Confident Its Shareholders Will Reject Mylan's Value Destructive Transaction 2Perrigo Confident Its Shareholders Will Reject Mylan's Value Destructive Transaction 3Perrigo Confident Its Shareholders Will Reject Mylan's Value Destructive Transaction 4Perrigo Confident Its Shareholders Will Reject Mylan's Value Destructive Transaction 5Korean Researchers Find the Possibility that Adult Mesenchymal Stem Cells (MSCs) May Extend Lifespan 2mHealth Market is Expected to Reach $49.12 Billion By 2020: Grand View Research, Inc. 2mHealth Market is Expected to Reach $49.12 Billion By 2020: Grand View Research, Inc. 3mHealth Market is Expected to Reach $49.12 Billion By 2020: Grand View Research, Inc. 4mHealth Market is Expected to Reach $49.12 Billion By 2020: Grand View Research, Inc. 5
... , SANTA ROSA, Calif. , Feb. 16 ... Osseoplasty™ procedures in Europe . The procedures were performed in ... Incorporado, SL, the largest independent medical device distributor in Europe ... weeks in the United Kingdom , Germany and ...
... , , CRANBURY, N.J. , ... today announced financial results for the fourth quarter and full year ... product pipeline related to its key strategic priorities for 2010.  Additionally, ... achieve its key milestone of completing enrollment for its Phase 3 ...
Cached Medicine Technology:Osseon Launches in Europe 2Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 2Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 3Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 4Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 5Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 6Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 7Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 8Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 9Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 10Amicus Therapeutics Announces Fourth Quarter and Full Year 2009 Financial Results and Highlights Product Pipeline Advancements 11
...
...
Solid blade speculum designed for both temporal and superior approach ocular surgery. Adjustable with thumb screw. Manufactured in titanium....
Solid 13.5 mm wide by 5 mm deep blades. Edge of blades serrated. Dull finish....
Medicine Products: