Navigation Links
Study reveals new metabolic safeguards against tumor cells
Date:8/19/2009

BOSTON, Mass. (August 19, 2009) Cells don't like to be alone. In the early stages of tumor formation, a cell might be pushed out of its normal home environment due to excessive growth. But a cell normally responds to this homeless state by dismantling its nucleus, packing up its DNA, and offering itself to be eaten by immune system cells. Simply put, the homeless cell kills itself. This process, known as apoptosis, typically stops potential cancer cells before they have a chance to proliferate.

Now, researchers from the lab of Harvard Medical School professor of cell biology Joan Brugge have uncovered another mechanism that kills these precancerous, homeless cells. By studying two different types of human breast epithelial cells, the researchers found that when separated from their natural environment, these cells lose their ability to harvest energy from their surroundings. Eventually, they starve.

"We originally thought that in order for cells to survive outside their normal environment, they would simply need to suppress apoptosis," says Brugge, senior author on the paper, which will appear August 19 online in Nature. "But our studies indicate that this activity is not sufficient to prevent the demise of homeless cells. Even if they escape apoptosis, these cells can't transport enough glucose to sustain an energy supply."

Surprisingly, metabolic function is restored if antioxidant activity is increased inside the cells, allowing the cells to use energy pathways that don't rely on glucose.

"It raises the interesting idea that antioxidants, which are typically thought to be protective because they prevent genomic damage, might be allowing these potentially dangerous cells to survive," says first author Zachary Schafer, assistant professor at the University of Notre Dame and a former postdoc in Professor Brugge's lab.

The authors caution against extrapolating too far from their data, which were based on experiments in laboratory cell culture. They also emphasize that the experiments were not designed to mimic the effect of dietary antioxidants in the body. The researchers used two specific antioxidant compoundswhich are chemically distinct from those found in food and supplementsonly in order to understand how oxidants contributed to the metabolic defects.

"We think that genes with antioxidant activity play a much bigger role than antioxidant compounds administered from outside the body," says Brugge. "What happens with dietary antioxidants is much more complicated and not what we were trying to study."

Beyond cell suicide

The researchers had previously reported that when cells were endowed with a cancer-causing gene that prevents them from committing suicide, they still died when cut off from their extracellular environment. This puzzled researchers, who have long thought that apoptosis was the only way the cells could die.

In the recent study, Schafer and colleagues took a closer look, measuring the levels of proteins and molecules associated with metabolic activity in the displaced, but apoptosis-resistant, cells. They found that the cells had become incapable of taking up glucose, their primary energy source. Under the microscope, the cells also displayed telltale signs of oxidative stress, a harmful accumulation of oxygen-derived molecules called reactive oxygen species (ROS). The end result was a halt in the production of ATP, the molecular lifeblood that transports energy in the cells. The unmoored cells were literally starving to death.

"The idea that a lack of extracellular matrix can prevent cells from accessing nutrients hasn't been shown conclusively before," says Schafer. "Loss of glucose transport, decreased ATP production, increased oxidative stressall those things turn out to be interrelated."

To figure out what was wrong, the researchers took a straightforward approachthey tried to fix it. Schafer engineered the homeless cells to express high levels of a gene, HER2, known to be hyperactive in many breast tumors. He also treated the cells with antioxidants in an attempt to relieve oxidative stress and help the cells survive.

Both strategies worked. The cells with the breast cancer gene regained glucose transport, preventing ROS accumulation, and recovered their ATP levels. The antioxidant-treated cells also survived, but by using fatty acids instead of glucose as an energy source.

"Our results raise the possibility that antioxidant activity might allow early-stage tumor cells to survive where they otherwise would die from these metabolic defects," says Schafer.

The researchers are currently planning to test the effects of antioxidant genes, some of which are abnormally regulated in human tumors, and a wider range of antioxidants in animal models. They also plan on characterizing the metabolic consequences of matrix detachment in more detail.

"Ultimately," Brugge says, "we want to understand enough about the metabolism of tumor cells so that new types of drugs can be designed to target them."


'/>"/>

Contact: David Cameron
david_cameron@hms.harvard.edu
617-432-0441
Harvard Medical School
Source:Eurekalert

Related medicine news :

1. Nonprofit nursing homes provide better care, major study finds
2. Longitudinal study investigates cocaines impact on adolescent development
3. Study finds adverse effects in treatment for primary sclerosing cholangitis
4. Study shows carvedilol is effective in preventing variceal bleeding in cirrhotic patients
5. Profiles International Releases New Study Identifying Key Factors for Strategic Workforce Planning
6. Thomson Reuters Study Finds Impact of Recession Easing on U.S. Hospitals
7. Retirees health-care benefits at risk, study warns
8. Lilly Reports on Outcome of Phase III Study of Arzoxifene
9. New study finds links between video-game playing and health risks in adults
10. Gen-Probe Begins U.S. Clinical Study of APTIMA(R) Trichomonas Vaginalis Assay on the TIGRIS(R) System
11. Study shows how to boost value of Alzheimers-fighting compounds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... (PRWEB) , ... June 26, 2016 , ... Pixel Film ... Pro X. , "Film editors can give their videos a whole new perspective by ... Austin - CEO of Pixel Film Studios. , ProSlice Levels contains over 30 ...
(Date:6/25/2016)... Washington, D.C. (PRWEB) , ... June 25, 2016 ... ... will discuss health policy issues and applications at AcademyHealth’s Annual Research Meeting June ... share their work on several important health care topics including advance care planning, ...
(Date:6/25/2016)... Lewisville, TX (PRWEB) , ... June 25, 2016 , ... ... in the United States, named Dr. Sesan Ogunleye, as the Medical Director of its ... be the facility Medical Director of our new Mesquite location,” said Dr. James M. ...
(Date:6/25/2016)... ... ... Conventional wisdom preaches the benefits of moderation, whether it’s a matter of ... too high can result in disappointment, perhaps even self-loathing. However, those who set the ... Research from PsychTests.com reveals that behind the tendency to set low expectations ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Marcy was in a crisis. Her son ... lash out at his family verbally and physically. , “When something upset him, he couldn’t ... would use it. He would throw rocks at my other children and say he was ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016 ... "Pharmaceutical Excipients Market by Type (Organic Chemical (Sugar, ... Formulation (Oral, Topical, Coating, Parenteral) - Global Forecast to ... The global pharmaceutical excipients market ... at a CAGR of 6.1% in the forecast period ...
(Date:6/23/2016)... -- Research and Markets has announced the addition ... to their offering. The ... commercial environment for MedImmune to enter. The US ageing population ... to drive considerable growth for effective anti-influenza medications. The introduction ... considerably, but development is still in its infancy. ...
(Date:6/23/2016)... 23, 2016 Revolutionary technology includes ... Oticon , industry leaders in advanced audiology and hearing ... Oticon Opn ™, the world,s first internet connected hearing ... IoT devices.      (Photo: http://photos.prnewswire.com/prnh/20160622/382240 ... number of ,world firsts,: , TwinLink™ - ...
Breaking Medicine Technology: