Navigation Links
Study Finds Blood Cells Can Be Reprogrammed to Act as Embryonic Stem Cells
Date:4/20/2009

WASHINGTON, April 20 /PRNewswire-USNewswire/ -- In a recent study, U.S. researchers have reprogrammed cells found in circulating blood into cells that are molecularly and functionally indistinguishable from embryonic stem cells, a revolutionary achievement that provides a readily accessible source of stem cells and an alternative to harvesting embryonic stem cells. The findings were prepublished online in Blood, the official journal of the American Society of Hematology.

Embryonic stem cells have long been coveted for their potential to treat a multitude of diseases as a result of their unique properties of nearly indefinite self-renewal and pluripotency (the ability to develop into any type of cell in the body), but their use has been the subject of political controversy.

"Our findings provide the first proof that cells from human blood can morph into stem cells," said senior study author George Q. Daley, M.D., Ph.D., an investigator for the Howard Hughes Medical Institute at Children's Hospital, Boston. "Making pluripotent stem cells from blood, which is one of the easiest tissues to obtain, provides an easy strategy for generating patient-specific stem cells that are valuable research tools and may one day be used to treat a number of diseases."

To generate induced pluripotent stem cells (dubbed iPS cells), blood was collected from a 26-year-old male donor. From the blood sample, the researchers isolated CD34+ cells, a type of stem cell that produces only blood cells, and cultured them in growth factors for six days to increase their number.

During the culture, the scientists infected the CD34+ cells with viruses carrying reprogramming factors, genes normally expressed in embryonic stem cells that can reset the blood cells to an embryonic state. Colonies of cells exhibiting physical characteristics similar to embryonic stem (ES) cells appeared about two weeks after the procedure. To determine whether these cells were also functionally similar to ES cells, the scientists analyzed the CD34+ iPS cell lines to see if they had acquired stem cell "markers," the unique combination of proteins that coat the cells' surface and distinguish them from other types of cells. Indeed, the iPS cell lines expressed the same markers as ES cells and further shared the capacity to differentiate into a variety of specialized cell types.

In vitro, the iPS cells readily developed into clusters of cells called embryoid bodies from which cells of virtually any type can develop. These differentiated cells expressed genes for all three embryonic germ layers (the tissues from which all other tissue types in the body develop) and also produced myeloid and granulocyte colonies (types of white blood cells).

The group confirmed that the reprogrammed cells had acquired ES cell characteristics by injecting the newly reprogrammed cells into immunodeficient mice. The cells successfully generated well-differentiated teratomas, benign masses containing all three embryonic germ layers, including respiratory, bone, and neural tissue.

"Not only has this work identified a new programmable cell type, but the cells are easy to obtain and analyze in many research laboratories and bone marrow transplantation centers around the world," said Grover C. Bagby, M.D., Professor of Medicine and Molecular and Medical Genetics at Oregon Health and Science University, who is not affiliated with the study. "These findings will immediately enhance the pace of laboratory research in this field and will ultimately help to determine whether iPS cells have a therapeutic potential equivalent to that of embryonic stem cells."

The American Society of Hematology (www.hematology.org) is the world's largest professional society concerned with the causes and treatment of blood disorders. Its mission is to further the understanding, diagnosis, treatment, and prevention of disorders affecting blood, bone marrow, and the immunologic, hemostatic, and vascular systems, by promoting research, clinical care, education, training, and advocacy in hematology. In September 2008, ASH launched Blood: The Vital Connection (www.bloodthevitalconnection.org), a credible online resource addressing bleeding and clotting disorders, anemia, and cancer. It provides hematologist-approved information about these common blood conditions including risk factors, preventive measures, and treatment options.

Blood, the official journal of ASH, is the most cited peer-reviewed publication in the field. Blood is issued to Society members and other subscribers weekly and is available in print and online at www.bloodjournal.org.


'/>"/>
SOURCE American Society of Hematology
Copyright©2009 PR Newswire.
All rights reserved

Related medicine news :

1. Penn study finds pro-death proteins required to regulate healthy immune function
2. New study shows promise in reducing surgical risks associated with surgical bleeding
3. Study, meta-analysis examine factors associated with death from heatstroke
4. Study suggests loss of 2 types of neurons -- not just 1 -- triggers Parkinsons symptoms
5. Study says COPD testing is not measuring up
6. Preclinical study suggests organ-transplant drug may aid in lupus fight
7. Ability to cope with stress can increase good cholesterol in older white men, study finds
8. High alcohol consumption increases stroke risk, Tulane study says
9. Mailman School of Public Health study examines link between racial discrimination and substance use
10. Pitt study finds inequality in tobacco advertising
11. Stanford study highlights cost-effective method of lowering heart disease risks
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/9/2016)... ... December 09, 2016 , ... "I have gout, and I wanted to treat ... which is meant to relieve gout and pain caused by varicose veins. I drank ... every time. It relieved what VA doctors called the worst sinusitis case they'd seen ...
(Date:12/9/2016)... ... 2016 , ... An inventor from Cana, Va., wanted to fulfill the need ... park rides. , The patent-pending SAFETY STRAP FOR AMPUTEES improves accessibility. It eliminates discrimination. ... set up in a matter of minutes, or even seconds. The SAFETY STRAP FOR ...
(Date:12/9/2016)... ... 2016 , ... MEDI+SIGN®, a provider of fully-automated patient monitoring ... for Emergency Departments (ED) has been added to their portfolio. Housed in a ... and with a simplified pallet of information available to the patient, the Digital ...
(Date:12/8/2016)... , ... December 08, 2016 , ... ... become Quality Insights beginning January 1, 2017. The name change aligns the ... to measuring and improving health care quality. , “We are very proud of ...
(Date:12/8/2016)... ... December 08, 2016 , ... ... Company LLC, announced the first national #QuackGivesBack campaign which supported local breast ... was our first franchise-wide Quack Gives Back initiative, and we’re very ...
Breaking Medicine News(10 mins):
(Date:12/8/2016)... CITY , Dec. 8, 2016 ... US patents for improving the accuracy, reproducibility and ... images in long and small bone orthopaedic applications. ... approach to creating personalized orthopaedic restorations based on ... personalized orthopaedic restorations, the company harnesses the world,s ...
(Date:12/8/2016)... 2016  A new study by a pair of ... opioid therapy to treat chronic pain is not only ... harmful consequences, including death. Palliative care physicians ... Mehta , M.D., authored the study which provides a ... The study was published in the December 2016 edition ...
(Date:12/8/2016)... December 8, 2016 Information products and services ... in Scopus , the world,s largest abstract and citation ... comprehensive metrics for journals from over 5,000 publishers. The new set ... to subscribe to and when to adjust a journal,s editorial strategy. ... , , ...
Breaking Medicine Technology: