Navigation Links
Sensor detects glucose in saliva and tears for diabetes testing
Date:8/23/2012

WEST LAFAYETTE, Ind. Researchers have created a new type of biosensor that can detect minute concentrations of glucose in saliva, tears and urine and might be manufactured at low cost because it does not require many processing steps to produce.

"It's an inherently non-invasive way to estimate glucose content in the body," said Jonathan Claussen, a former Purdue doctoral student and now a research scientist at the U.S. Naval Research Laboratory. "Because it can detect glucose in the saliva and tears, it's a platform that might eventually help to eliminate or reduce the frequency of using pinpricks for diabetes testing. We are proving its functionality."

Claussen and Purdue doctoral student Anurag Kumar led the project, working with Timothy Fisher, a Purdue professor of mechanical engineering, D. Marshall Porterfield, a professor of agricultural and biological engineering, and other researchers at the university's Birck Nanotechnology Center.

Findings are detailed in a research paper appearing online this week in the journal Advanced Functional Materials.

"Most sensors typically measure glucose in blood," Claussen said. "Many in the literature aren't able to detect glucose in tears and the saliva. What's unique is that we can sense in all four different human serums: the saliva, blood, tears and urine. And that hasn't been shown before."

The paper, featured on the journal's cover, was written by Claussen, Kumar, Fisher, Porterfield, and Purdue researchers David B. Jaroch, M. Haseeb Khawaja, and Allison B. Hibbard.

The sensor has three main parts: layers of nanosheets resembling tiny rose petals made of a material called graphene, which is a single-atom-thick film of carbon; platinum nanoparticles; and the enzyme glucose oxidase.

Each petal contains a few layers of graphene stacked on each other. The edges of the petals have dangling, incomplete chemical bonds, defects where the platinum nanoparticles can attach. Electrodes are formed by combining the nanosheet petals and platinum nanoparticles. Then the glucose oxidase attaches to the platinum nanoparticles. The enzyme converts glucose to peroxide, which generates a signal on the electrode.

"Typically, when you want to make a nanostructured biosensor you have to use a lot of processing steps before you reach the final biosensor product," Kumar said. "That involves lithography, chemical processing, etching and other steps. The good thing about these petals is that they can be grown on just about any surface, and we don't need to use any of these steps, so it could be ideal for commercialization."

In addition to diabetes testing, the technology might be used for sensing a variety of chemical compounds to test for other medical conditions.

"Because we used the enzyme glucose oxidase in this work, it's geared for diabetes," Claussen said. "But we could just swap out that enzyme with, for example, glutemate oxidase, to measure the neurotransmitter glutamate to test for Parkinson's and Alzheimer's, or ethanol oxidase to monitor alcohol levels for a breathalyzer. It's very versatile, fast and portable."

The technology is able to detect glucose in concentrations as low as 0.3 micromolar, far more sensitive than other electrochemical biosensors based on graphene or graphite, carbon nanotubes and metallic nanoparticles, Claussen said

"These are the first findings to report such a low sensing limit and at the same time such a wide sensing range," he said.

The sensor is able to distinguish between glucose and signals from other compounds that often cause interference in sensors: uric acid, ascorbic acid and acetaminophen, which are commonly found in the blood. Unlike glucose, those compounds are said to be electroactive, which means they generate an electrical signal without the presence of an enzyme.

Glucose by itself doesn't generate a signal but must first react with the enzyme glucose oxidase. Glucose oxidase is used in commercial diabetes test strips for conventional diabetes meters that measure glucose with a finger pinprick.
'/>"/>

Contact: Emil Venere
venere@purdue.edu
765-494-4709
Purdue University
Source:Eurekalert  

Related medicine news :

1. Football helmet sensors help researchers demystify concussion in young athletes
2. Doubt Cast on Usefulness of Sensory Therapies for Autism
3. Heart failure patients with diabetes may benefit from higher glucose levels
4. Skp2 activates cancer-promoting, glucose-processing Akt
5. Aggressively controlling glucose levels may not reduce kidney failure in Type 2 diabetes
6. Glucose deprivation activates feedback loop that kills cancer cells, UCLA study shows
7. QuickMedical® Offers The WaveSense Blood Glucose Monitoring System
8. Saliva test could dramatically increase detection of oral cancer
9. Stem cell sparing radiotherapy for head and neck cancer may avoid salivary gland damage
10. Researchers aim to grow salivary glands using patients own cells
11. How a cancer drug leads to diabetes
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Sensor detects glucose in saliva and tears for diabetes testing
(Date:5/26/2017)... , ... May 26, 2017 , ... ... first ever copper, antimicrobial, mesh back 24/7 task chair specifically designed for clinical ... “We are thrilled to partner with Cupron® to provide customers with a ...
(Date:5/26/2017)... ... ... Silver Birch of Hammond, a new assisted lifestyle community, has welcomed its first ... at 5620 Sohl Avenue in Hammond, serves older adults who need some help to ... apartments. Each of the private apartments at Silver Birch features a kitchenette, spacious bathroom ...
(Date:5/26/2017)... ... 2017 , ... “THE FLINTHILLS FAMILY-Our Journey to the Cross”: the personal journey of Bob Massey ... the creation of published authors, Bob and Margaret Massey. Bob Massey is small in ... quick and leather tough." His love for others is apparent in all of his ...
(Date:5/26/2017)... , ... May 26, 2017 , ... Water damage to ... New Jersey School District had left education officials with a number of critical issues ... of the flooring had to be accomplished with little or no disruption to class ...
(Date:5/24/2017)... ... May 24, 2017 , ... Altec Products, Inc., a leader ... – a one-day technology conference in San Diego, CA. , At nVerge 2017, ... to fully utilize and enhance their Sage ERP solutions by providing improved visibility and ...
Breaking Medicine News(10 mins):
(Date:5/10/2017)... , May 10, 2017 CSSi, the global ... the clinical research industry, is proud to announce the ... The new website features both enriched content and a ... and enhances the company,s already well-established position as the ... "After many months of hard work, we ...
(Date:5/9/2017)... -- Oramed Pharmaceuticals Inc. (NASDAQ: ORMP ) ... on the development of oral drug delivery systems, ... has granted Oramed a patent titled, "Methods and ... covers Oramed,s invention of an oral glucagon-like peptide-1 ... hormone that stimulates the secretion of insulin from ...
(Date:5/8/2017)... May 8, 2017  Diplomat Pharmacy, Inc. (NYSE: DPLO)., ... ("WRB"), a health care service center company based ... WRB specializes in relationship management programs for leading pharmaceutical ... WRB will join Envoy Health ... services for manufacturers, biotech firms, and other service companies. ...
Breaking Medicine Technology: