Navigation Links
Sensor detects glucose in saliva and tears for diabetes testing
Date:8/23/2012

WEST LAFAYETTE, Ind. Researchers have created a new type of biosensor that can detect minute concentrations of glucose in saliva, tears and urine and might be manufactured at low cost because it does not require many processing steps to produce.

"It's an inherently non-invasive way to estimate glucose content in the body," said Jonathan Claussen, a former Purdue doctoral student and now a research scientist at the U.S. Naval Research Laboratory. "Because it can detect glucose in the saliva and tears, it's a platform that might eventually help to eliminate or reduce the frequency of using pinpricks for diabetes testing. We are proving its functionality."

Claussen and Purdue doctoral student Anurag Kumar led the project, working with Timothy Fisher, a Purdue professor of mechanical engineering, D. Marshall Porterfield, a professor of agricultural and biological engineering, and other researchers at the university's Birck Nanotechnology Center.

Findings are detailed in a research paper appearing online this week in the journal Advanced Functional Materials.

"Most sensors typically measure glucose in blood," Claussen said. "Many in the literature aren't able to detect glucose in tears and the saliva. What's unique is that we can sense in all four different human serums: the saliva, blood, tears and urine. And that hasn't been shown before."

The paper, featured on the journal's cover, was written by Claussen, Kumar, Fisher, Porterfield, and Purdue researchers David B. Jaroch, M. Haseeb Khawaja, and Allison B. Hibbard.

The sensor has three main parts: layers of nanosheets resembling tiny rose petals made of a material called graphene, which is a single-atom-thick film of carbon; platinum nanoparticles; and the enzyme glucose oxidase.

Each petal contains a few layers of graphene stacked on each other. The edges of the petals have dangling, incomplete chemical bonds, defects where the platinum nanoparticles can attach. Electrodes are formed by combining the nanosheet petals and platinum nanoparticles. Then the glucose oxidase attaches to the platinum nanoparticles. The enzyme converts glucose to peroxide, which generates a signal on the electrode.

"Typically, when you want to make a nanostructured biosensor you have to use a lot of processing steps before you reach the final biosensor product," Kumar said. "That involves lithography, chemical processing, etching and other steps. The good thing about these petals is that they can be grown on just about any surface, and we don't need to use any of these steps, so it could be ideal for commercialization."

In addition to diabetes testing, the technology might be used for sensing a variety of chemical compounds to test for other medical conditions.

"Because we used the enzyme glucose oxidase in this work, it's geared for diabetes," Claussen said. "But we could just swap out that enzyme with, for example, glutemate oxidase, to measure the neurotransmitter glutamate to test for Parkinson's and Alzheimer's, or ethanol oxidase to monitor alcohol levels for a breathalyzer. It's very versatile, fast and portable."

The technology is able to detect glucose in concentrations as low as 0.3 micromolar, far more sensitive than other electrochemical biosensors based on graphene or graphite, carbon nanotubes and metallic nanoparticles, Claussen said

"These are the first findings to report such a low sensing limit and at the same time such a wide sensing range," he said.

The sensor is able to distinguish between glucose and signals from other compounds that often cause interference in sensors: uric acid, ascorbic acid and acetaminophen, which are commonly found in the blood. Unlike glucose, those compounds are said to be electroactive, which means they generate an electrical signal without the presence of an enzyme.

Glucose by itself doesn't generate a signal but must first react with the enzyme glucose oxidase. Glucose oxidase is used in commercial diabetes test strips for conventional diabetes meters that measure glucose with a finger pinprick.
'/>"/>

Contact: Emil Venere
venere@purdue.edu
765-494-4709
Purdue University
Source:Eurekalert  

Related medicine news :

1. Football helmet sensors help researchers demystify concussion in young athletes
2. Doubt Cast on Usefulness of Sensory Therapies for Autism
3. Heart failure patients with diabetes may benefit from higher glucose levels
4. Skp2 activates cancer-promoting, glucose-processing Akt
5. Aggressively controlling glucose levels may not reduce kidney failure in Type 2 diabetes
6. Glucose deprivation activates feedback loop that kills cancer cells, UCLA study shows
7. QuickMedical® Offers The WaveSense Blood Glucose Monitoring System
8. Saliva test could dramatically increase detection of oral cancer
9. Stem cell sparing radiotherapy for head and neck cancer may avoid salivary gland damage
10. Researchers aim to grow salivary glands using patients own cells
11. How a cancer drug leads to diabetes
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Sensor detects glucose in saliva and tears for diabetes testing
(Date:2/5/2016)... ... February 05, 2016 , ... The event is being ... Block Event Center in Minneapolis, Minn. Triumph Over Parkinson’s will fund nearly $100,000 for ... of Schneiderman’s Furniture, lives with Parkinson’s disease and is the architect of this informative ...
(Date:2/5/2016)... ... , ... After years as an active staff surgeon and having served as ... Wayne Carman transitioned to chief of the Division of Plastic Surgery at what is ... chief and began a second three-year term in January of 2016. , The original ...
(Date:2/5/2016)... ... February 05, 2016 , ... Regular gym users know ... having to wait longer to access the treadmills. It’s a predictable trend. After the ... lose weight and get in shape by joining gyms, starting new walking or running ...
(Date:2/5/2016)... Georgia (PRWEB) , ... February 05, 2016 , ... Dr. ... to announce their 2nd Annual No Cost Dental Day to individuals in need. The ... The purpose of this No Cost Dental Day is to provide dental care to ...
(Date:2/5/2016)... ... February 05, 2016 , ... Health and wellness is a topic that should ... event they are experiencing an illness. Migraines are a severe form of a headache ... migraines would not wish the pain on their worst enemy, the feeling can last ...
Breaking Medicine News(10 mins):
(Date:2/5/2016)... , Feb. 5, 2016  Aralez Pharmaceuticals Inc. ... of POZEN Inc. ("POZEN") and Tribute Pharmaceuticals Canada Inc. ("Tribute") ... and shareholders of Tribute. The combined company will operate ... company with operations in Canada , ... United States . Under the terms of the ...
(Date:2/5/2016)... February 5, 2016 --> ... report states that the global active pharmaceuticals ingredients (APIs) ... predicted to reach US$185.9 bn by 2020. It is ... 2014 to 2020. The title of the report is ... by Geography, and by Therapeutic Area) - Global Industry ...
(Date:2/5/2016)... Feb. 5, 2016  Henry Schein, Inc. (NASDAQ: HSIC ... and services to office-based dental, animal health and medical ... agreement to acquire a majority ownership interest in Dental ... in Brazil . ... Dental Cremer is the dental distribution business of Cremer ...
Breaking Medicine Technology: