Navigation Links
Selected cells from blood or bone marrow may provide a route to healing blood vessels
Date:8/13/2010

Isolating cells from a patient's blood or bone marrow that nourish blood vessels may be a safer and less arduous route to treatment of cardiovascular disease than obtaining rare stem cells, according to research from Emory University School of Medicine.

In recent clinical trials, doctors in several countries have tested the ability of a patient's bone marrow cells to repair damage, such as heart attacks and peripheral artery disease, created by problems of blood flow.

"The focus has been on stem cells, but it looks like the main beneficial effects come from transplanted cells' ability to support the growth of nearby blood vessels," says senior author Young-sup Yoon, MD, PhD, associate professor of medicine (cardiology) at Emory University School of Medicine. "Based on this idea, we wanted to identify a population of cells enriched with the capacity to regenerate blood vessels."

The blood vessel-repairing properties of selected cells from human blood were described in the Aug. 10 issue of the Journal of the American College of Cardiology, with a related paper on cells derived from mouse bone marrow published online July 15 by the journal Circulation Research.

The first author of the JACC paper is postdoctoral fellow Sung-Whan Kim, PhD. The first author of the Circulation Research paper is Hyongbum Kim, MD, PhD, now an assistant professor in Korea.

Yoon's team focused on the molecule CD31, also known as platelet endothelial cell adhesion molecule-1 or PECAM-1, because of its presence on endothelial cellsthe cells that form the inner lining of blood vessels. In experiments with donated blood from human volunteers or mouse bone marrow cells, the researchers showed that cells with CD31 on their surfaces secrete hormones that support the growth of blood vessels.

About a third of the cells in the blood or bone marrow have CD31 on their surfaces, including some differentiated immune cells. In culture, sorted cells displaying CD31 can form tubular structures mimicking the growth of blood vessels in the body.

"We can show that after transplantation, some CD31 positive cells do become endothelial cells, but their main effect is more to support other cells than to become the building blocks," Yoon says.

The researchers used antibodies against CD31 to sort human blood or mouse bone marrow cells into two groups: cells with CD31 and those without. They then tested these cells' ability to spur blood vessel regrowth in mice whose hind legs had a blocked blood supply.

In the project described in Circulation Research, after two weeks more than 80 percent of the mouse hind legs transplanted with CD31 positive bone marrow cells survived, while less than 15 percent of the legs transplanted with CD31 negative cells survived. In laser Doppler images, the mice with CD31 positive cells injected into their legs had greatly enhanced blood flow and an increased number of capillaries.

Yoon says harvesting CD31 positive cells may have several advantages compared to previous methods of treating cardiovascular disease. The cells can be prepared without the need to grow them in a dish for several days, and it may not be necessary to take large volumes of blood or bone marrow from the patientan advantage with respect to safety. In addition, cells from mice used to simulate atherosclerosis (mutant for a gene that helps clear fat from the blood and given a high-fat diet) do not seem to lose their repair potential.

"Based on the insights gained from preclinical and clinical studies from several investigators, we view the use of CD31 positive cells as a second-generation cardiovascular cell therapy that could be a novel option for the treatment of peripheral artery disease," Yoon says.

He adds that CD31 positive cells may have potential for treating other conditions, including heart attack, heart failure and diabetic neuropathy, which his team is investigating in animal models.


'/>"/>

Contact: Holly Korschun
hkorsch@emory.edu
404-727-3990
Emory University
Source:Eurekalert

Related medicine news :

1. Nurse-Attorney/Former Bristol-Myers Squibb Executive Selected To Lead New Jersey State Nurses Association
2. Keenan Chief Strategy Officer Selected to Join Editorial Board of Payers & Providers
3. The Icebox of Atlanta Selected as T-shirt Vendor for 3 Major Divisions of American Cancer Society's Relay For Life
4. Nurtur Is Selected By Keenan & Associates as Exclusive Provider of Disease Management Programs
5. Small Time Company Rolls into the Big League: Trigger Point Performance Therapy Selected to Attend Athletes' Performance NFL Combine Preparation Program
6. Rajesh Khanna, MD Selected as One of America's Top Ophthalmologist
7. DC Dermatologist Dr. Susan T. Elliott Selected Best Doctor by Washingtonian Magazine
8. PrescriptionDrugs.com and Mike “Zappy” Zapolin Have Been Selected to Compete in Harvard Business School New Venture Contest
9. Dr. Rajesh Khanna Selected as Best Lasik Surgeon Two Years in a Row
10. R. Shane Jackson Selected as Panelist for Center for Health Transformation Member Meeting
11. Phoseon Technology Selected as Finalist for 2010 TechAmerica Oregon Technology Awards
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... ... ... of Bruton Memorial Library on June 21 due to a possible lice infestation, as reported ... head lice: the parasite’s ability to live away from a human host, and to infest ... in the event that lice have simply gotten out of control. , As lice are ...
(Date:6/25/2016)... ... June 25, 2016 , ... As a lifelong Southern Californian, ... and his M.D from the David Geffen School of Medicine at UCLA. He trained ... Angeles to complete his fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program where ...
(Date:6/25/2016)... ... 25, 2016 , ... Conventional wisdom preaches the benefits of moderation, whether it’s ... setting the bar too high can result in disappointment, perhaps even self-loathing. However, those ... goal. , Research from PsychTests.com reveals that behind the tendency to ...
(Date:6/24/2016)... , ... June 24, 2016 , ... June 19, 2016 ... dangers associated with chronic pain and the benefits of holistic treatments, Serenity Recovery ... are suffering with Sickle Cell Disease. , Sickle Cell Disease (SCD) is a disorder ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Global law ... magazine’s 2016 Legal Elite. The attorneys chosen by their peers for this recognition are ... , Seven Greenberg Traurig Shareholders received special honors as members of this year’s Legal ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... 24, 2016  Consumers have taken a more ... placed more emphasis on patient outcomes. ... in the pharmaceutical industry have evolved beyond just ... companies are focusing on becoming more patient-oriented across ... and services that improve health. ...
(Date:6/24/2016)... , June 24, 2016   Bay ... Rehabilitation Network,s Dean Center for Tick Borne ... Medicine and Rehabilitation, MIT Hacking Medicine, University of ... Innovation, today announced the five finalists of ... Lyme disease.  More than 100 scientists, clinicians, researchers, ...
(Date:6/24/2016)... , June 24, 2016 The Academy ... of recommendations that would allow biopharmaceutical companies ... with entities that make formulary and coverage decisions, a ... "value" of new medicines. The recommendations address ... not appear on the drug label, a prohibition that ...
Breaking Medicine Technology: