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Seeing through HIV's disguises
Date:2/27/2013

ns important to the virus' disease activity.

After isolating HIV-1 particles from lab-grown human CD4+ T-cells, Graham and his team used powerful protein sequencing and bioinformatics tools to identify all of the associated human proteins. They then used the same bioinformatics tools to re-analyze protein content information from HIV-1 particles isolated from lab-grown human macrophages, data previously published by another group of researchers.

With the help of sophisticated computing, the team identified 279 proteins taken up by HIV-1 particles from one or the other cell type. Of these, only 25 were shared by viruses from both cell types.

One protein the research team identified that could be particularly important to diagnosis and treatment is CD44, because it appears to be the only one of the 25 capable of binding to other cells. It helps the viruses attach themselves to sites of inflammation. Graham suggests that "this makes a lot of sense for a virus that likes to infect T-cells and macrophages because both types of cells migrate to sites of inflammation to help out. Little do they know that HIV viruses are lying in wait."


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Contact: Catherine Kolf
ckolf@jhmi.edu
443-287-2251
Johns Hopkins Medicine
Source:Eurekalert  

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