Navigation Links
Seaweed and fireflies brew may guide stem cell treatment for peripheral artery disease
Date:3/10/2009

An unlikely brew of seaweed and glow-in-the-dark biochemical agents may hold the key to the safe use of transplanted stem cells to treat patients with severe peripheral arterial disease (PAD), according to a team of veterinarians, basic scientists and interventional radiologists at Johns Hopkins.

In a preliminary "proof of concept" study in rabbits, Johns Hopkins scientists safely and successfully delivered therapeutic stem cells via intramuscular injections and then monitored the stem cells' viability once they reached their targets.

A report of the study by Johns Hopkins radiologists is scheduled for presentation at the Society of Interventional Radiology's 34th annual scientific meeting March 10.

Stem cells hold promise in treating PAD by reconstituting or increasing the number of blood vessels to replace or augment those choked off by plaque buildup. A chronic condition that can lead to amputations and even death, PAD is marked by vastly reduced circulation of blood in vessels feeding the legs and other "peripheral" body parts, and affects as many as 10 million Americans. Many cases can be treated with angioplasty or stents, similar to approaches used in coronary artery disease, but for some patients with extensive disease conventional treatment is not feasible, researchers say.

Among the technical hurdles to improving blood flow in such patients, according to Dara L. Kraitchman, V.M.D., Ph.D., associate professor of radiology at Johns Hopkins, is a means of telling doctors whether injected stem cells are staying alive and reaching the right targets to grow and develop into the needed new tissue.

This is critical, Kraitchman says, because the body's own immune defenses may recognize the potentially helpful donor stem cells as foreign invaders and try to destroy them, and also because traditional radioactive labeling agents, or tracers, which are normally used to track cells, can be toxic to stem cells.

To overcome rejection of the stem cells by the body's immune system in this case, rabbit immune systems they first created a novel "capsule" derived from seaweed, which was used to surround and protect the rabbit stem cells from attack by the host's immune system. Within the seaweed capsule, they added X-ray contrast agents to allow the capsules to be seen on X-ray angiography. Next, they engineered the stem cells within the capsules to produce luciferase, the same bioluminescent chemical produced by fireflies, which is highly visible under bioluminescence imaging.

"Once we were able to trick the immune system into not attacking the cells, we had to know they arrived at their destination and were living," says Kraitchman. "We could use standard X-ray angiography of blood vessels to see the transplanted cells. When they lit up like fireflies at night, we knew they were still alive."

"Hopefully, this new technology will one day pave the way for treating humans," says Frank Wacker, M.D., director of vascular interventional radiology at Hopkins and visiting professor of radiology. "We look to the day when we will be able to perform targeted delivery of stem cell to treat PAD in patients who may be facing amputation or death."


'/>"/>

Contact: Gary Stephenson
gstephenson@jhmi.edu
410-955-5384
Johns Hopkins Medical Institutions
Source:Eurekalert

Related medicine news :

1. Fireflies glow helps UT Southwestern researchers track cancer drugs effectiveness
2. FRC Responds to President Obamas Misguided Decision on Stem Cell Science
3. HEROES Grants, Education Guide Support Youth Leaders Addressing Childhood Obesity Epidemic
4. The REACH Institute: Best Practice Guidelines Address Mental Health Needs of Youth in Care
5. New Book Exposes Junk Science, Conflicts of Interest, Disease Guidelines Affecting Millions
6. IPRO QIO Uses Milliman Care Guidelines(R) for Medicare Reviews in New York State
7. American College of Gastroenterology Issues Updated Colorectal Cancer Guidelines
8. New CME guidelines advise paradigm shift in physician education
9. Recovery Audit Contractor HealthDataInsights Licenses Milliman Care Guidelines(R)
10. American Academy of Dermatology Issues New Guidelines For The Management of Psoriasis With Topical Therapies
11. New tool guides doctors to save cancer patients fertility
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/26/2016)... ... May 26, 2016 , ... Metcalf & Associates’ Maureen ... of experience in leading technology and human resources operations for health care, education, ... will be featured on Metcalf’s VoiceAmerica radio show , Innovative Leaders Driving ...
(Date:5/26/2016)... ... 2016 , ... May 2016 – Lips may be a central feature on ... Record numbers of clients now ask about lip plumping and primping techniques and want ... of Advanced Dermatology P.C. , The trend is a national one, with ...
(Date:5/26/2016)... ... May 26, 2016 , ... ... to announce the launch of its brand equity product, Brandvantage. This breakthrough ... the dynamic landscape of modern consumer decision-making. The proprietary framework incorporates attitudes, ...
(Date:5/26/2016)... ... May 26, 2016 , ... Development Team of Pixel Film Studios ... , "This new layered style transition tool will keep a consistent flow to your ... Pixel Film Studios. , TranSweep is an all new layered style transition tool ...
(Date:5/26/2016)... Madeira Beach, Florida (PRWEB) , ... May 26, 2016 , ... ... island connected to the mainland by a single drawbridge, citizens formed an organization, Madeira ... increase already congested traffic and change the small town center to a high rise ...
Breaking Medicine News(10 mins):
(Date:5/25/2016)... GERMANTOWN, Maryland , May 25, 2016 /PRNewswire/ ... dringenden Bedarf zur Steuerung ... N.V. (NASDAQ: QGEN ; Frankfurt Prime ... Entwicklungsvereinbarung mit Therawis Diagnostics GmbH zur Entwicklung und ... sein. Ein erstes Projekt wird die Entwicklung und ...
(Date:5/25/2016)... 2016  According to Kalorama Information, the world ... 2015.  Though these are challenging times in the ... success for companies that remain optimistic and seek ... growth prospects medical device companies spend a higher ... (R&D) than do companies in other industries.  Also, ...
(Date:5/25/2016)... , May 25, 2016  Granger Diagnostics today announced ... for wounds and infections. This test ensures discovery ... select viruses. The test requires only a simple swab ... David G. Bostwick , MD, Chief ... facilitate wound healing: "We are excited to make ...
Breaking Medicine Technology: