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Seaweed and fireflies brew may guide stem cell treatment for peripheral artery disease
Date:3/10/2009

An unlikely brew of seaweed and glow-in-the-dark biochemical agents may hold the key to the safe use of transplanted stem cells to treat patients with severe peripheral arterial disease (PAD), according to a team of veterinarians, basic scientists and interventional radiologists at Johns Hopkins.

In a preliminary "proof of concept" study in rabbits, Johns Hopkins scientists safely and successfully delivered therapeutic stem cells via intramuscular injections and then monitored the stem cells' viability once they reached their targets.

A report of the study by Johns Hopkins radiologists is scheduled for presentation at the Society of Interventional Radiology's 34th annual scientific meeting March 10.

Stem cells hold promise in treating PAD by reconstituting or increasing the number of blood vessels to replace or augment those choked off by plaque buildup. A chronic condition that can lead to amputations and even death, PAD is marked by vastly reduced circulation of blood in vessels feeding the legs and other "peripheral" body parts, and affects as many as 10 million Americans. Many cases can be treated with angioplasty or stents, similar to approaches used in coronary artery disease, but for some patients with extensive disease conventional treatment is not feasible, researchers say.

Among the technical hurdles to improving blood flow in such patients, according to Dara L. Kraitchman, V.M.D., Ph.D., associate professor of radiology at Johns Hopkins, is a means of telling doctors whether injected stem cells are staying alive and reaching the right targets to grow and develop into the needed new tissue.

This is critical, Kraitchman says, because the body's own immune defenses may recognize the potentially helpful donor stem cells as foreign invaders and try to destroy them, and also because traditional radioactive labeling agents, or tracers, which are normally used to track cells, can be toxic to stem cells.

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Contact: Gary Stephenson
gstephenson@jhmi.edu
410-955-5384
Johns Hopkins Medical Institutions
Source:Eurekalert

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